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UnknownAn improved synthesis of a fluorophosphonate-polyethylene glycol-biotin probe and its use against competitive substrates.
Not available
S-EPMC3566859 | biostudies-literature
ProteomicsSerine Hydrolases from E. Coli
Serine hydrolases from E. Coli cells were labelled with fluorophosphonate probe (ABP-probe): Intact cells labelling by adding probe to media: GPM77700012901 and GPM00300015270 Cell lysate labelling: GPM77700012904 and GPM77700012905 Blank sample (no probe addition): GPM77700012906 and GPM77700012909...
ORGANISM(S): Escherichia coli 
2014-05-20 | PXD000241 | Pride
Activity-based protein profiling E. coli Serine hydrolases Fluorophosphonate probe 
UnknownProfiling Serine Hydrolases in the Leishmania Host-Pathogen Interactome Using Cell-Permeable Activity-Based Fluorophosphonate Probes.
Not available
S-EPMC12117450 | biostudies-literature
UnknownNewly discovered Staphylococcus aureus serine hydrolase probe and drug targets.
Not available
S-EPMC8957240 | biostudies-literature
ProteomicsMFGE8 links absorption of dietary fatty acids with catabolism of enterocyte lipid stores through HNF4y dependent transcription of CES enzymes
Fluorophosphonate probe experiments for enterocytes from WT mice, Mfge8 KO mice and Hnf4y KO mice.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-04-24 | MSV000089304 | MassIVE
ProteomicsDruggable Serine Proteases of Leishmania by Activity‐Based Protein Profiling
Leishmaniasis are a group of diseases caused by parasitic protozoa of the genus Leishmania. Current treatments are limited by difficult administration, high cost, poor efficacy, toxicity, and growing resistance. New agents, with new mechanisms of action, are urgently needed to treat the disease. Alt...
ORGANISM(S): Leishmania mexicana 
2022-07-04 | PXD033616 | Pride
Activity-based protein profiling Leishmania Serine protease 
ProteomicsActivity-Based Protein Profiling of Human and Plasmodium Serine Hydrolases and Interrogation of Potential Antimalarial Targets
Malaria remains a global health issue requiring the identification of novel therapeutic targets to combat drug resistance. Metabolic serine hydrolases are druggable enzymes playing essential roles in lipid metabolism. However, very few have been investigated in malaria-causing parasites. Here, we us...
ORGANISM(S): Homo sapiens (Human) Plasmodium falciparum (isolate 3D7) 
2022-10-14 | PXD035891 | Pride
Abp Abpp Plasmodium falciparum Serine hydrolase Malaria Chemical proteomics Fluorophosphonate 
ProteomicsFP-biotin enrichment of serine hydrolases in Enterococcus faecium
In our study, we employed activity-based protein profiling (ABPP), a technique that uses specialized inhibitors to identify active serine hydrolases in different strains of E. faecium (clade A1 and A2) and E. lactis under various growth conditions. Serine hydrolases, a large and diverse family of en...
ORGANISM(S): Enterococcus faecium Bacteria 
2024-06-22 | PXD048798 | Pride
Lc-msms Enterococcus faecium Activity-based protein profiling Fp-biotin Serine hydrolases 
ProteomicsIn vivo labelling of sediments from Kamchatka Peninsula springs with FP-alkyne
This experiment aims on the identification of serine hydrolases from a complex thermophile community that live in a hot vent in Kamchatka Peninsula based on in vivo labelling with FP-alkyne directly in the hot spring and subsequent analysis using metagenomics/metaproteomics. To this end, sediment sa...
ORGANISM(S): hot springs metagenome 
2024-06-03 | PXD025833 | Pride
Abpp Kamtchatka Fp Fluorophosphonate Metagenome 
ProteomicsInvestigation of xylan degradation in Thermococcus sp. 2319x1E
Thermococcus sp. 2319x1E is able to grow on different mono- or polysaccharides as carbon source, including xylan. To investigate xylan degradation in Thermococcus sp. 2319x1E, cells were grown on 4 different carbon sources for 12 h (mid-exponential phase) and harvested by centrifugation. Using a lab...
ORGANISM(S): unclassified Thermococcus 
2022-02-17 | PXD026056 | Pride
Abpp Thermococcus sp 2319x1e Fp Fluorophosphonate 
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