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ProteomicsAP-MS analysis of human FUBIeS30/FAU WT and non-cleavable G73,4A and G74V mutants
To discover candidate FUBIeS30 proteases, we performed affinity purification from human cell lysates expressing tandem Strep II-hemagglutinin (StHA)-tagged WT or mutant (G73,74A or G74V) FUBIeS30-StHA constructs. Subsequent analysis of the co-purified proteins by mass spectrometry (LC-MS/MS) identif...
ORGANISM(S): Homo sapiens (Human) 
2021-08-05 | PXD021864 | Pride
Fubies30 Fau Ap-ms Dda Dub 
ProteomicsAP-MS analysis of human RIOK1 and USP16
To discover novel factors involved in the final steps of 40S ribosomal subunit biogenesis in human cells, we isolated late cytoplasmic 40S subunit precursors by affinity purification (AP) of the assembly factor RIOK1. Subsequent analysis of their composition by mass spectrometry (MS) led to identifi...
ORGANISM(S): Homo sapiens (Human) 
2020-03-04 | PXD016458 | Pride
Usp16 Ap-ms Riok1 Dda 
ProteomicsThe GTPase Nog1 co-ordinates assembly, maturation and quality control of distant ribosomal functional centers
Yeast ribosome assembly analysis, using APMS on various 60S tagged subunits (SSF1, RIX1, ARX1, KRE35, ARX1_bud20D, KRE35_DRG1, KRE35_drg-dn, YEP, G223, NOG1)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-01-06 | PXD011382 | Pride
Ribosome Apms S cerevisiae 60s 
ProteomicsCRISPRi meets metabolomics: a platform for rapid functional annotation of compound libraries
Molecular profiling of small-molecules offers invaluable insights on compound functionality and allows for hypothesis generation of targets. However, current profiling methods are either limited in the number of measurable parameters or throughput. Here, we developed a multiplexed, unbiased framewor...
ORGANISM(S): Escherichia coli 
2021-12-16 | PXD024133 | Pride
Proteomics Escherichia coli Metabolomics Lc-ms Antibacterial drugs Limited proteolysis 
ProteomicsDynamic 3D proteomes reveal functional pathway alterations in situ
Many biological processes are regulated via molecular events, such as intermolecular interactions and chemical modifications, which do not affect protein levels and escape detection in classical proteomic screens. Reasoning that these events affect protein structure, we propose here that a global pr...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2020-11-02 | PXD022297 | Pride
Lc-msms Heat shock Metabolism Dia Limited proteolysis Structural barcodes Osmotic stress Dda Structure-function Shotgun proteomics 
ProteomicsYeast proteomic analysis upon temperature shift
DIA/SWATH analysis of yeast upon temperature shift.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-28 | PXD016320 | Pride
Proteomics Dia S cerevisiae Swath Puf6 Yeast 
ProteomicsProteomic profiling to predict therapeutic response to anti-PD1 therapy in advanced melanoma – SWATH MS dataset - glycopeptides
MS-based proteomics based on data-independent acquisition / SWATH MS was used to study the response of melanoma patients to anti-PD1 therapy. Human serum samples were collected from patients at the start of their therapy and later categorized into responders and non-responders based on clinical outc...
ORGANISM(S): Homo sapiens (Human) 
2023-08-21 | PXD027933 | Pride
Melanoma  serum  immunodepletion  drug response  therapeutic efficacy  anti-pd1 therapy  biomarker discovery 
ProteomicsSWATH Analysis of Yeast Proteome over Time in Response to Osmotic Stress
SWATH analysis of Yeast Proteome over time in response to Osmotic Stress. We sampled cell cultures in biological triplicates at six time points following the application of osmotic stress and acquired single injection DIA datasets on a high-resolution 5600 tripleTOF instrument operated in SWATH mode...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2017-11-03 | MSV000081677 | MassIVE
OtherReproducible and consistent quantification of the Saccharomyces cerevisiae proteome by SWATH-MS.
Data from ProteomeXchange, PXD ID: PXD001010. File: nselevse_L120203_020-Yeast OGE 11- 4 ul - IDA20-150ms.mgf. Published as part of Mol Cell Proteomics. 2015 Jan 5 . From the Abstract: {{i}} ... We sampled cell cultures in biological triplicates at six time points following the application of osmot...
ORGANISM(S): Yeast 
GPM32320007153 | GPMDB
Resource Reanalysis 
ProteomicsSWATH analysis of 1433B_HUMAN affinity purifications from HEK293 cells after time course treatment with IGF1
Protein complexes and protein interaction networks are essential mediators of most biological functions. Complexes supporting transient functions such as signal transduction processes are frequently subject to dynamic remodeling. Currently, the majority of studies into the composition of protein com...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-20 | MSV000082833 | MassIVE
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