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ProteomicsUSP9X phosphopeptide mapping - We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732). The functional connection of these proteins has been further investigated by a quantitative comparison of USP9X phosphorylation in WT vs CDC14B-overexpressing HEK 293Tcells (ATCC® CRL-3216™). The approach identified USP9X serine 2563 as a specific CDC14B target. Phosphorylation at USP9XS2563 has been confirmed by in-house phosphospecific antibodies.
We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732). The functional connection of these proteins has been further investigated by a quantitative comparison of USP9X phosphorylation in WT vs CDC14B-overexpressing HEK 293Tcells (ATCC® CRL-3216™). The appr...
ORGANISM(S): Homo sapiens (Human) 
2020-03-25 | PXD012733 | Pride
Cdc14b Usp9x 
ProteomicsCDC14B interactome - The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively based on spectral counting. This approach yielded the deubiquitylase USP9X as a G2/M-specific CDC14B interactor which has been confirmed by Western-blot (WB).
The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively bas...
ORGANISM(S): Homo sapiens (Human) 
2020-03-25 | PXD012732 | Pride
Usp9x Cdc14b 
ProteomicsIdentification of mitotic substrates of phospho-regulated USP9X
We previously identified the deubiquitylase USP9X as a G2/M-specific CDC14B interactor (PXD012732) and subsequently identified USP9X as a specific CDC14B target (PXD012733). To investigate relevant mitotic substrates of phospho-regulated USP9X, we next performed a Stable isotope labeling by amino ac...
ORGANISM(S): Homo sapiens (Human) 
2020-03-25 | PXD012734 | Pride
Usp9x Ubiquitinylation 
ProteomicsMapping of nanobody binding sites on human LRRK2 by CX-MS
As part of an in-depth characterization of nanobodies directed against the human Leucine-rich repeat kinase 2 (LRRK2), epitopes of the nanobodies bound to LRRK2 were mapped by chemical crosslinking combined with mass spectrometry using the CID-cleavable crosslinker disuccinimidyl sulfoxide (DSSO).
ORGANISM(S): Homo sapiens (Human) 
2022-02-27 | PXD030063 | Pride
Cl-ms Lrrk2 Dsso Nanobodies Cx-ms Crosslinking Cid-cleavable 
ProteomicsMapping of TDP-43 ubiquitination and acetylation sites
TAR DNA binding protein of 43 kDa (TDP-43) forms aggregates in neurodegenerative diseases, particularly certain forms of frontotemporal dementia and amyotrophic lateral sclerosis. Pathological modifications of TDP-43 include proteolytic fragmentation, phosphorylation, and ubiquitinylation. In this s...
ORGANISM(S): Homo sapiens (Human) 
2022-04-01 | PXD030170 | Pride
Acetylation Ubiquitination Tdp-43 
ProteomicsAnalysis of conformational changes induced by binding of allosteric nanobodies to a bacterial LRRK2 homologue by CX-MS
As part of an in-depth characterization of nanobodies directed against the Chlorobium tepidum protein CtRoco, a bacterial LRRK2 homologue, chemical crosslinking combined with mass spectrometry (CX-MS) was performed. Two allosteric nanobodies binding to two different epitopes in the LRR and the Roc d...
ORGANISM(S): Chlorobium tepidum 
2024-04-23 | PXD046634 | Pride
Lrrk2 Dsso Nanobodies Crosslinking Ctroco 
ProteomicsUsher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins
The human Usher syndrome (USH) is the most common form of a sensory hereditary ciliopathy characterized by progressive vision and hearing loss. Mutations in the genes ADGRV1 and CIB2 have been associated with two distinct sub-types of USH, namely USH2C and USH1J. The proteins encoded by the two gene...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042629 | Pride
Usher syndrome Tap Interactome 
UnknownFlower morphology as a predictor of pollination mode in a biotic to abiotic pollination continuum.
Not available
S-EPMC10550269 | biostudies-literature
UnknownIntramolecular feedback regulation of the LRRK2 Roc G domain by a LRRK2 kinase-dependent mechanism.
Not available
S-EPMC11658767 | biostudies-literature
UnknownA visual review of the interactome of LRRK2: Using deep-curated molecular interaction data to represent biology.
Not available
S-EPMC4415485 | biostudies-literature
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