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ProteomicsLFQ proteomics analysis of M. smegmatis treated with BacPROTACs.
To monitor selective degradation of ClpC1 and ClpC2 using BacPROTAC compounds in M. smegmatis we performed a LFQ proteomics analysis treating the cells at bactericidal compound concentration. We detected degradation of both proteins and additional dysregulation of the mycobacterial PQC system.
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037235 | Pride
Antibiotics Tpd Bacprotac Protac Protein degradation 
UnknownNegative regulation of G2-M by ATR (mei-41)/Chk1(Grapes) facilitates tracheoblast growth and tracheal hypertrophy in Drosophila.
Not available
S-EPMC5953539 | biostudies-literature
ProteomicsLFQ proteomics analysis of the M. smegmatis ClpC2 interactome.
To assess the ClpC2 interactome, a IP-MS analysis was performed using ectopically expressed tagged ClpC2 in a ClpC2 deficient cell line. To evaluate potential binding sites on ClpC2, CymA treatment was applied to compete with potential interactors for a shared ClpC2 binding site.
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037231 | Pride
Antibiotics Tpd Protein degradation 
UnknownCytochrome c oxidase dysfunction enhances phagocytic function and osteoclast formation in macrophages.
Not available
S-EPMC6662975 | biostudies-literature
ProteomicsA quantitative proteomics approach to monitor targeted protein degradation in M. smegmatis
Targeted protein degradation via the ubiquitin proteasome has been established in eukaryotes, however no application has been shown in bacteria so far. In the present work, selective protein degradation is shown using hetero bifunctional drugs, bringing substrates in close proximity to the bacterial...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) Bacteria 
2022-08-12 | PXD021505 | Pride
Mycobacteria Targeted protein degradation Protease Tpd 
UnknownInteraction of p-benzoquinone with hemoglobin in smoker's blood causes alteration of structure and loss of oxygen binding capacity.
Not available
S-EPMC5615826 | biostudies-literature
ProteomicsLFQ proteomics analysis of M. smegmatis treated with CymA and ecumicin (Part 1).
To assess the impact of the bactericidal compounds CymA and ecumicin on the mycobacterial proteome and to monitor proteome remodeling by the AAA+ ClpC1 protease machinery, we performed a LFQ proteomics analysis treating the cells at sub-bactericidal compound concentration. We identified new players ...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037232 | Pride
Tuberculosis Tpd Protein degradation 
ProteomicsLFQ proteomics analysis of M. smegmatis treated with CymA and ecumicin (Part 2).
To assess the impact of the bactericidal compounds CymA and ecumicin on the mycobacterial proteome and to monitor proteome remodeling by the AAA+ ClpC1 protease machinery, we performed a LFQ proteomics analysis treating the cells at bactericidal compound concentration. We identified new players of t...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-07 | PXD037234 | Pride
Antibiotics Tuberculosis Tpd Protein degradation 
ProteomicsA quantitative phosphoproteomic analysis to study the dynamics of tyrosine phosphorylation in EGFR signaling abrogated by TKIs
Lung cancer is the leading cause of cancer death. Mutations in the kinase domain of EGFR, a predominant driver oncogene, such as L858R missense mutation and a series of deletions spanning the conserved sequence 747LREA750, are associated with sensitivity to tyrosine kinase inhibitors (TKIs). However...
ORGANISM(S): Homo sapiens (Human) 
2017-03-29 | PXD004373 | Pride
Nsclc Phosphoproteomics Tki Egfr Adenocarcinoma 
ProteomicsOrbitrap proteomic analysis of five distinct molecular subtypes of High Grade Serous Ovarian Carcinoma (HGSOC) under serum deprived and serum fed conditions
Cell lines representing five distinct phenotypes of high-grade serous ovarian carcinoma (HGSOC), including Epithelial (E), Intermediate-Epithelial (iE), Epithelial-Mesenchymal Hybrid (E/M), Intermediate-Mesenchymal (iM), and Mesenchymal (M), previously classified in our laboratory based on the expre...
ORGANISM(S): Homo sapiens (Human) 
2025-10-08 | PXD062888 | Pride
Serum starvation A4 Caov3 Ovarian cancer Ovcar3 High grade serous ovarian carcinoma (hgsoc) Ovca420 Ovmz6 
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