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ProteomicsAn HDX-MS optimised approach to characterise nanobodies as tools for biochemical and structural studies
Given the far-reaching applications of nanobodies, a stream-lined approach to characterizing nanobodies tailored to its intended application is warranted. Camelids can produce a large number of antibodies that can bind to a host of epitopes spanning the entire protein of interest, making identificat...
ORGANISM(S): Homo sapiens (Human) 
2021-09-22 | PXD025207 | Pride
Hdx-ms Pi3k Nanobody 
ProteomicsHDX-MS of TRAPPII reveals a conserved Rab binding interface
We used hydrogen deuterium exchange mass spectrometry (HDX-MS) to define the interface of TRAPPII with three Rab GTPases (Rab1, Rab11, Rab43). These experiments were performed in the presence of EDTA to generate a nucleotide free stabilised Rab-GEF complex. Significant decreases in deuterium incorpo...
ORGANISM(S): Homo sapiens (Human) 
2020-12-11 | PXD020890 | Pride
Hdx-ms Trappii Gef Rab 
ProteomicsDefining the molecular mechanism of PI4KA complex regulation by the phosphatase Calcineurin
To examine the interaction between PI4KIIIa and Calcineurin, HDX-MS experiments comparing calcineurin or PI4KA FAM delta C alone to the calcineurin and PI4KA FAM delta C complex were carried out.
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD043409 | Pride
Hdx-ms Pi4k Calcineurin 
ProteomicsHDX-MS analysis of PI4Ka trimer and FAM126a (415-521) with calcineurin
We used HDX-MS to map novel binding sites of calcineurin on PI4KA and FAM126A. Calcineurin binds to PxIxIT and LxVP motifs on PI4KA and FAM126A indicating a novel regulatory mechanism of Pi4KA by calcineurin
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD050633 | Pride
Hdx-ms Pi4ka Fam126a Calcineurin 
ProteomicsHydrogen Deuterium Exchange of PI4K(530-560) Peptide with Calcineurin
we used HDXMS to study if PI4KA(530-560) is able to bind calcineurin. Here we showed a significant protection in deuterium exchange upon binding calcineurin in the wild type state, indicating these proteins are interacting. Using a mutant that should inhibit the interaction (AKASAA), we showed that ...
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD044636 | Pride
Hdxms Pi4k Calcineurin 
ProteomicsTargeting of disease-linked mutations in PI3K gamma
Using hydrogen deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics we have identified mutation specific conformational changes, and provide a molecular framework for how they alter kinase activity.Many of the activating conformational changes mimicked previously described changes th...
ORGANISM(S): Homo sapiens (Human) 
2021-03-26 | PXD021132 | Pride
Molecular dynamics Kinase assay Hdx-ms Inhibitor Pi3k gamma Pi3k 
ProteomicsHDX-MS Analysis of the PI4K EFR3A Complex
HDX-MS analysis examining the complex that forms between the subunits of PI4KIIIa and EFR3A
ORGANISM(S): Homo sapiens (Human) 
2025-03-10 | PXD043442 | Pride
Hdx-ms Pi4ka Efr3a Fam126a Ttc7b 
ProteomicsDetailing the Structural and Dynamic Interactions Between WDR44 and Rab11
DR44 is a Rab11 effector protein that is thought to regulate ciliogenesis by competing with pro-ciliogenesis factors for Rab11 binding. The structure of WDR44 and the molecular mechanism of its interaction with Rab11 is unknown. To explore the interactions between these two proteins multiple WDR44 c...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD035741 | Pride
Hdx-ms Wdr44 Rab11 
ProteomicsDetailing the Structural and Dynamic Interactions Between WDR44 and Rab11
Analysis of the interactions between WDR44 and Rab11 utilizing HDX-MS
ORGANISM(S): Homo sapiens (Human) 
2023-02-22 | PXD032837 | Pride
Hdx-ms Wdr44 Rab11 
ProteomicsAllosteric activation or inhibition of PI3Kγ mediated through conformational changes in the p110γ helical domain
To provide additional insight into the molecular mechanisms underlying p110 phosphorylation we carried out hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments on p110 and phosphorylated p110 (90.8% phosphorylated S594/595, 92% phosphorylated S582. When we compared phosphorylated p110 ...
ORGANISM(S): Homo sapiens (Human) 
2023-07-06 | PXD040765 | Pride
Pi3k Hdx-ms Phosphorylation Hdx 
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