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TranscriptomicsRNA-Seq of control, RNPS1 knockdown and rescue in HeLa cells
We report the knockdown and rescue of RNPS1, a component of the ASAP and PSAP complexes and, hence, an auxiliary component of the exon junction complex. The studied conditions show an extensive change in alternative splicing and the cell's transcriptome. For the knockdown, HeLa cells were transfect...
ORGANISM(S): Homo sapiens 
2018-11-01 | E-MTAB-6564 | biostudies-arrayexpress
ProteomicsLC-MSMS based Phosphoproteomics of BubR1
To identify phosphorylation sites on BubR1 that regulates its acetylation at lysine 250 (K250), LC-MSMS analysis was performed using HeLa cells expressing wild-type BubR1 (WT), an acetylation-deficient mutant (K250R), and an acetylation-mimetic mutant (K250Q).
ORGANISM(S): Homo sapiens (Human) 
2025-12-22 | PXD066850 | Pride
Bubr1 Hela Phosphorylation 
ProteomicsMitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism
1. Sample ID: 1005942, 1005943 - Description: The samples are HeLa cells transfected with pcDNA-FRT-T0 containing DLAT-HA. They were used to investigate whether NADK2 is required for DLAT lipoylation. 2. Sample ID: 1187969-1187973 - Description: The samples are HeLa cells transfected with pcDNA-FRT...
ORGANISM(S): Homo sapiens (Human) 
2025-03-03 | PXD060981 | Pride
Hadph Mitochondrial fatty acid synthesis Protein lipolyation 
ProteomicsIdentification of BRCA2-DSS1 new partner HELZ
R-loops are dynamic three-stranded nucleic acid structures that regulate genome function but can threaten genome stability if not properly resolved. BRCA2, a key player in homologous recombination (HR), also regulates R-loops, though the mechanisms remain unclear. To uncover novel R-loop regulators,...
ORGANISM(S): Homo sapiens (Human) 
2025-12-30 | PXD066766 | Pride
Hela Biotinid-dss1 Brca2 Helz 
ProteomicsCancer proteome and metabolite changes linked to SHMT2
To screen for proximate and interacting proteins of SHMT2 in HeLa cells, the BioID method was employed, which consists of biotinylation of proteins in the vicinity of FlagBirAR118G –SHMT2 followed by streptavidin-based affinity capture and mass spectrometry identification. Experimental procedures we...
ORGANISM(S): Homo sapiens (Human) 
2020-08-12 | PXD004629 | Pride
Streptavidin Shmt2 Ms Bioid 
ProteomicsThe interactome of a family of potential methyltransferases
Human methytransferase like proteins (METTL) are part of a large protein family characterized by the presence of binding domains for S-adenosyl methionine, a co-substrate in most methylation reactions. Despite the fact that members of this protein family were shown or predicted to be DNA, RNA or pro...
ORGANISM(S): Homo sapiens (Human) 
2019-05-06 | PXD011125 | Pride
Gfp affinity purifications Interaction proteomics Mass spectrometry Mettl 
ProteomicsMethyltransferase METTL5 interactome
Cellular RNAs are covalently modified and these modifications can impact on all biological processes and hence are implicated in different types of diseases. Amongst RNA modifications, N6-methyladenosine (m6A) is one of the most widespread and has been found on messenger (mRNA), ribosomal (rRNA), no...
ORGANISM(S): Homo sapiens (Human) 
2020-09-09 | PXD015582 | Pride
Hela Rna methyltranferase Interactome 
ProteomicsProteomic analysis identifies novel BRCA1-associated protein 1 binding proteins
BRCA1-associated protein 1 (BAP1) is a tumor suppressor and its loss can result in mesothelioma, uveal and cutaneous melanoma, clear cell renal cell carcinoma and bladder cancer. BAP1 is a deubiquitinating enzyme of the UCH class that has been implicated in various cellular processes like cell growt...
ORGANISM(S): Homo sapiens (Human) 
2021-09-21 | PXD023676 | Pride
Hela Ap-ms Bap1 Deubiquitination Lc-ms Proteomics 
UnknownNovel Template Plasmids pCyaA'-Kan and pCyaA'-Cam for Generation of Unmarked Chromosomal cyaA' Translational Fusion to T3SS Effectors in Salmonella.
Not available
S-EPMC7996335 | biostudies-literature
UnknownAssessing cell-specific effects of genetic variations using tRNA microarrays.
Not available
S-EPMC6632033 | biostudies-literature
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