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UnknownDevelopment of an Online 2D Ultrahigh-Pressure Nano-LC System for High-pH and Low-pH Reversed Phase Separation in Top-Down Proteomics.
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S-EPMC7544661 | biostudies-literature
ProteomicsDifferential proteome analysis of Escherichia coli grown on acetate vs. glucose
Relative protein abundances of Escherichia coli growing exponentially on minimal medium with acetate or glucose as the sole carbon source were investigated in a quantitative shotgun proteome analysis with TMT6-plex isobaric tags. Peptides were separated by high resolution high/low pH 2D-LC, using an...
ORGANISM(S): Escherichia coli 
2016-10-11 | PXD003863 | Pride
Tmt Isobaric High/low ph 2d-lc 
ProteomicsDevelopment of an online 2D high-pH and low-pH reversed-phase nanoLC-MS/MS system for comprehensive proteome analysis
In bottom-up proteomics, the complexity of proteome profiling is usually limited by the number of identified proteins in nanoflow LC MS/MS analysis. Herein, we developed a fully automatic online 2D nanoLC MS/MS system using both high-pH and low-pH RPLCs for a comprehensive proteomics analysis.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-06-05 | MSV000089609 | MassIVE
ProteomicsProteomics in biomanufacturing control: Protein dynamics of CHO-K1 cells and conditioned media during apoptosis and necrosis
Cell viability has a critical impact on product quantity and quality during the biomanufacturing of therapeutic proteins. An advanced understanding of changes in the cellular and conditioned media proteomes upon cell stress and death is therefore needed for improved bioprocess control. Here, a high ...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2018-02-27 | PXD008297 | Pride
Proteomics Cho Keywords: biomanufacturing Necrosis Apoptosis 2d-lc-mse 
ProteomicsProteomic Analysis of Human Liver Reference Material
Recent advances in mass spectrometric (MS) instruments resulting in high mass resolution and high duty cycles have allowed of very deep coverage of proteomes. However, even with state of the art, high duty cycle instruments, the number of proteins identified with a conventional single liquid chromat...
ORGANISM(S): Homo sapiens (Human) 
2018-04-23 | PXD009021 | Pride
Human Liver 
ProteomicsProfiling of fasting-feeding dynamics in mouse liver
Daily biological rhythms are orchestrated by a combination of the intrinsic circadian clock and external food/feeding-related signals which influence metabolism in health and disease. Understanding how and to which extent both circadian and fasting/feeding rhythms contribute to regulating daily phys...
ORGANISM(S): Mus musculus (Mouse) 
2021-01-22 | PXD008944 | Pride
Metabolism Mouse Fasting Liver 1d-lc-msms Online 2d-lc-msms Feeding 
ProteomicsCombination of bottom up 2D-LC-MS and intact protein separation enhances coverage of proteome and low molecular weight short open reading frame encoded peptides of the archeon Methanosarcina mazei
The recent discovery of an increasing number of small open reading frame (sORF) creates the need for suitable analytical technologies for the comprehensive identification of the corresponding gene products. In the present study we evaluated analytical approaches which will allow for simultaneous ana...
ORGANISM(S): Methanosarcina mazei Go1 
2016-09-26 | PXD004325 | Pride
Gelfree High ph lc High ph 2d-lc Top-down Methanosarcina mazei 
ProteomicsQuantitative differences in rumen epithelium proteins, as well as body composition and metabolizable energy intake in lambs fed a low or high energy diet.
The rumen is a major the site of digestion and absorption of energy predominantly in the form of short chain fatty acids as well as amino acids and simple carbohydrates. The research sought to determine if diets high or low in ME were associated with quantitative changes in the membrane bound protei...
ORGANISM(S): Ovis aries 
2026-02-09 | PXD054700 | Pride
Epithelium Nutrient Transport Rumen Membrane 
ProteomicsOnline-2D nanoLC-MS for crude serum proteome profiling: assessing sample preparation impact on proteome composition
Although current LC-MS technology permits scientists to efficiently screen clinical samples in translational research, e.g. steroids, biogenic amines and even plasma or serum proteomes, in a daily routine, maintaining the balance between throughput and analytical depth is still a limiting factor. A ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-09 | PXD024893 | Pride
Peptide fractionation Blood Nanolc-ms 1d nanolc-ms Proteome profiling Crude serum High-resolution accurate mass-spectrometry Online-2d nanolc-ms 
ProteomicsTrimodal chromatography enables facile peptide fractionation
We evaluated the utility of a trimodal stationary phase (Trinity P1) in offline peptide fractionation, and found it performed similarly to ion exchange or high pH reversed phase based techniques. It is orthogonal to low pH reversed phase and uses MS compatible mobile phase solvents.
ORGANISM(S): Homo sapiens (Human) 
2017-08-07 | PXD006188 | Pride
Peptide fractionation Proteomics Trinity p1 Orthogonality 2d-lc Pancreatic cancer 
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