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ProteomicsSCFCyclin F targets the metabolic protein Sirtuin 5 for ubiquitination
Cell cycle transitions are regulated by a number of coordinating signaling events. The ubiquitin-proteasome system is an important part of this cell cycle signaling. Cyclin F, a substrate adapter for the SCF (SKP1/CUL1/F-box) E3 ubiquitin ligase, is an important regulator of cell cycle progression. ...
ORGANISM(S): Homo Sapiens (human) 
2020-11-17 | PXD019396 | JPOST Repository
ProteomicsAblation of Sirtuin5 in the postnatal mouse heart results in persistent accumulation of protein succinylation and normal survival in response to chronic pressure overload
Mitochondrial Sirtuin 5 (SIRT5) is an NAD+-dependent demalonylase, desuccinylase, and deglutarylase that controls several metabolic pathways. A number of recent studies point to SIRT5 desuccinylase activity being important in maintaining cardiac function and metabolism under stress. Previously, we d...
ORGANISM(S): Mus musculus (Mouse) 
2018-05-16 | PXD008728 | Pride
Protein succinylation Heart function Sirt5 
ProteomicsLipids reprogram cellular metabolism to become a major carbon source for histone acetylation.
From McDonnell et. al. Cell Reports 2016: Cells integrate nutrient sensing and metabolism to coordinate proper cellular responses to a particular nutrient source. For example, glucose drives a gene expression program characterized by activating genes involved in its metabolism, in part, by increasi...
ORGANISM(S): Mus musculus (Mouse) 
2016-12-22 | PXD005040 | Pride
Lipid Proteomics Histone acetylation 
ProteomicsStatin therapy inhibits Fatty Acid Synthase via dynamic protein modifications
Recent discoveries revealed HMG-CoA is a reactive metabolite that can non-enzymatically modify proteins and impact their activity. Therefore, we predicted that inhibition of HMGCR by statins might increase HMG-CoA levels and protein modifications. Upon statin treatment, we observed a strong increase...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2022-05-20 | PXD030952 | Pride
Hmgylation Statin Fatty acid synthase 
ProteomicsA class of reactive acyl-CoA species reveals the nonenzymatic origins of protein acylation
The mechanisms underlying the formation of acyl protein modifications remain poorly understood. By investigating the reactivity of endogenous acyl-CoA metabolites, we found a class of acyl-CoAs that undergoes intramolecular catalysis to form reactive intermediates which non-enzymatically modify prot...
ORGANISM(S): Mus musculus (Mouse) 
2017-04-11 | PXD005895 | Pride
Glutarylation Hmgylation Nonenzymatic protein acylation 
ProteomicsNicotinamide Mononucleotide Requires SIRT3 to Improve Cardiac Function and Bioenergetics in a Friedreich’s Ataxia Cardiomyopathy Model
Martin et al. JCI Insigh (2017). Increasing NAD+ levels by supplementing with the precursor nicotinamide mononucleotide (NMN) improves cardiac function in multiple mouse models of disease. While NMN influences several aspects of mitochondrial metabolism, the molecular mechanisms by which increased N...
ORGANISM(S): Mus musculus (Mouse) 
2017-07-21 | PXD006754 | Pride
Nmn Friedreich’s ataxia Heart failure Sirt3 Acetylation 
ProteomicsSIRT4 Is a Lysine Deacylase that Controls Leucine Metabolism and Insulin Secretion
Sirtuins are NAD+-dependent protein deacylases that regulate several aspects of metabolism and aging. In contrast to the other mammalian sirtuins, the primary enzymatic activity of mitochondrial sirtuin 4 (SIRT4) and its overall role in metabolic control has remained enigmatic. Using a combination o...
ORGANISM(S): Mus musculus (Mouse) 
2017-04-05 | PXD005896 | Pride
Protein acylation Insulin secretion Leucine metabolism Sirt4 
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