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ProteomicsOff-line high pH reversed-phase fractionation for in-depth phosphoproteomics
In this study we provide a detailed analysis of the physicochemical characteristics of phosphopeptides, which have been fractionated by off-line high pH chromatography (HpH) before subsequent titanium dioxide (TiO2) enrichment and LC-MS/MS analysis. Our results demonstrate that HpH is superior to st...
ORGANISM(S): Mus musculus (Mouse) 
2014-10-23 | PXD001404 | Pride
Phosphoproteomics  high ph reversed-phase  phosphorylation  peptides  enrichment  fractionation  titanium dioxide  orbitrap 
ProteomicsThe impact of exercise on skeletal muscle proteome of prediabetic subjects analyzed with data independent mass spectrometry.
Skeletal muscle is a primary tissue responsible for both the exercise capacity and insulin-induced glucose uptake. Exercise-evoked proteomic alternations in muscle of subjects with prediabetes are of great importance for the elucidation of the connections between insulin resistance and physical exe...
ORGANISM(S): Homo sapiens (Human) 
2025-07-22 | PXD055536 | Pride
Gas-phase fractionation Gpf Dia Prediabetes Exercise Homo sapiens Skeletal muscle High-ph fractionation Hph 
ProteomicsComprehensive evaluation of different TiO2-based phosphopeptide enrichment and fractionation methods for phosphoproteomics
We performed a comprehensive evaluation of four different TiO2-based phosphopeptide enrichment methods using different non-phosphopeptide excluders. Then, two phosphopeptide fractionation methods, including the ammonia-based and the TEA based high pH reversed-phase fractionation, are also evaluated.
ORGANISM(S): Homo Sapiens 
2022-06-02 | PXD034264 |
ProteomicsComparison of protein and peptide fractionation approaches in protein identification and quantification from Saccharomyces cerevisiae
Proteomics is a high-throughput technology which has been developed with the aim of investigating the maximum number of proteins in cells in a given experiment. However, protein discovery and data generation vary in depth and coverage when different technical strategies are selected. In this study, ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-11-30 | PXD018854 | Pride
High ph reversed phase fractionation Sds-page Gpf Yeast Fasp Proteome 
ProteomicsHigher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer -fractionation
High-throughput proteomics is critical for understanding biological processes, enabling large-scale studies such as biomarker discovery and systems biology. However, current mass spectrometry technologies face limitations in speed, sensitivity, and scalability for analyzing large sample cohorts. The...
ORGANISM(S): Homo sapiens (Human) 
2026-02-16 | PXD066659 | Pride
Hph fractionation Orbitrap astral zoom 
UnknownComprehensive Evaluation of Different TiO2-Based Phosphopeptide Enrichment and Fractionation Methods for Phosphoproteomics.
Not available
S-EPMC9265536 | biostudies-literature
ProteomicsDeep Exploration of the Immunopeptidome of a Pancreatic Cancer Cell Line Panc1: Implications for Clinical Immunopeptidomics and Immunotherapy (DDA datasets)
Pancreatic ductal adenocarcinoma (PDAC), with its devastating prognosis and limited treatment options, necessitates innovative therapeutic strategies. T-cell-based immunotherapy has shown promise for various cancers, including PDAC. Thus, a comprehensive understanding of the HLA-peptidome in PDAC is...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD054360 | Pride
Tumor-associated antigens Hla Variable window zenoswath dia Pancreatic cancer Immunopeptidome Pdac 
ProteomicsDeep Exploration of the Immunopeptidome of a Pancreatic Cancer Cell Line Panc1: Implications for Clinical Immunopeptidomics and Immunotherapy (SWATH-DIA datasets)
Pancreatic ductal adenocarcinoma (PDAC), with its devastating prognosis and limited treatment options, necessitates innovative therapeutic strategies. T-cell-based immunotherapy has shown promise for various cancers, including PDAC. Thus, a comprehensive understanding of the HLA-peptidome in PDAC is...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD054417 | Pride
Tumor-associated antigens Hla Variable window zenoswath dia Pancreatic cancer Immunopeptidome Pdac 
ProteomicsProteomic analysis of human dentin matrix extracts by peptide-level high-pH reverse-phase fractionation
Proteomics research has increasingly focused on human cells, tissues, and fluids; however, comprehensive data regarding dental tissues remain limited. Dentin, a mineralized component of the tooth, contains structural proteins and bioactive molecules that, upon release, can modulate pulp cell acti...
ORGANISM(S): Homo sapiens (Human) 
2026-03-23 | PXD070849 | Pride
Dentin  dentin matrix  proteomics  extracellular matrix  mass spectrometry  regenerative endodontics 
UnknownDeep Exploration of the Immunopeptidome of a Pancreatic Cancer Cell Line: Implications for Clinical Immunopeptidomics and Immunotherapy.
Not available
S-EPMC12362687 | biostudies-literature
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