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ProteomicsExtending the Compatibility of the SP3 Paramagnetic Bead Processing Approach for Proteomics
The broad variability in protein and peptide biochemistry necessitates robust protocols and reagents for efficiently handling these molecules prior to analysis with mass spectrometry (MS) or other techniques. In this study, further exploration of the paramagnetic bead-based approach, Single-Pot Soli...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008698 | Pride
Hilic Tmt Mass spectrometry Sp3 Protein clean-up 
ProteomicsEfficient Parsing and Quantification of Native Mass Spectrometer Files with RawQuant
Effective analysis of protein samples by mass spectrometry (MS) instrumentation requires careful selection and optimization of a range experimental parameters. As the output from the primary detection device, the ‘raw’ MS data file can be used to gauge the success of a given sample analysis. However...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-05-07 | PXD008787 | Pride
Isobaric labeling Sps-ms3 Tmt Quantitative proteomics Orbitrap Itraq 
ProteomicsCDK12 interaction and knockdown global proteomics
Determining interaction partners with CDK12 using IP-MS. Capturing global proteome expression changes as a result of CDK12 knockdown.
ORGANISM(S): Homo sapiens (Human) 
2017-03-21 | PXD004184 | Pride
Ip-ms Cdk12 Shotgun proteomics 
ProteomicsMulti-omic analyses reveal interaction networks linking CIC to cell cycle regulation
CIC encodes a transcriptional repressor inactivated by loss-of-function mutations in several cancer types, indicating that it may function as a tumor suppressor. Recent data indicate that CIC may regulate cell cycle genes in humans; however, a thorough investigation of this proposed role has not yet...
ORGANISM(S): Homo sapiens (Human) 
2023-06-30 | PXD030942 | Pride
Protein-protein interaction Genetic interaction network Cell cycle Cic Single-cell sequencing 
ProteomicsA MYCN-independent mechanism mediating secretome reprogramming and metastasis in MYCN-amplified neuroblastoma
MYCN amplification (MNA) is a defining feature of high-risk neuroblastoma (NB) that predicts poor prognosis. However, whether genes within or in close proximity to the MYCN amplicon also contribute to aggressiveness in MNA+ NB remains poorly understood. Here we identify that GREB1, a transcription f...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039410 | Pride
Mycn Greb1 Myo1b Neuroblastoma Secretome 
ProteomicsCLK IP-MS - CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor
CLK kinase was immunoprecipitated using differential tagging of the N- and C-terminus
ORGANISM(S): Homo sapiens (Human) 
2017-03-14 | PXD003839 | Pride
Kinase Immunoprecipitation Tandem mass tag 
ProteomicsIL1RAP augments cysteine metabolism to promote ferroptosis resistance and metastasis in Ewing sarcoma
Aggressive cancer cells must adapt and overcome oxidative stress encountered during the metastatic cascade to successfully disseminate. In this work, a combination of proteomic approaches is used to identify the IL1 receptor accessory protein (IL1RAP) as an in vitro suppressor of anoikis (detachment...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-05-25 | PXD019009 | Pride
Tmt Cysteine metabolism Il1rap Ewing sarcoma 
ProteomicsLKB1 regulates JNK-dependent stress signaling and apoptotic dependency of KRAS-mutant lung cancers
KRAS-mutant lung cancers frequently have inactivating mutations in LKB1, which enhances tumor growth, metastasis, and immune evasion. This study shows that LKB1 suppresses JNK stress signaling, and its loss in KRAS-mutant cells leads to JNK hyperactivation and apoptotic rewiring, making them sensiti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-03-06 | MSV000097246 | MassIVE
UnknownWhole genome sequence analyses of brain imaging measures in the Framingham Study.
Not available
S-EPMC5772158 | biostudies-literature
UnknownChallenges of one-year longitudinal follow-up of a prospective, observational cohort study using an anonymised database: recommendations for trainee research collaboratives.
Not available
S-EPMC6909648 | biostudies-literature
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