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ProteomicsMultiplexed, Proteome-wide Protein Expression Profiling: Yeast Deubiquitylating Enzyme Knockout Strains
Characterizing a protein’s function often requires a description of the cellular state in its absence. Multiplexing in mass spectrometry-based proteomics has now achieved the ability to globally measure protein expression levels in yeast from 10 cell states simultaneously. We applied this approach t...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-12-08 | PXD003033 | Pride
Cox complex Isobaric labeling Tmt Deubiquitinases Ubp3 Quantitative proteomics Ubiquitin High throughput proteomics Orbitrap fusion Inorganic phosphate pathway. 
ProteomicsHighly Multiplexed Quantitative Mass Spectrometry Analysis of Ubiquitylomes
Systems-wide quantitative analysis of ubiquitylomes has proven to be a valuable tool for elucidating targets and mechanisms of the ubiquitin-driven signaling systems, as well as gaining insights in neurodegenerative diseases and cancer. Current mass spectrometry methods for ubiquitylome detection re...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-10-10 | PXD004628 | Pride
Ubiquitin Orbitrap fusion lumos tribrid mass spectrometer Mithocondria Bortezomib 10-plex tmt Mouse 
ProteomicsPhospho-proteomic characterization of CCDC6-RET
Here we show that a CCDC6-RET fusion product, a driver and therapeutic target in lung and thyroid cancers, is a highly active dimeric kinase in solution. Time-resolved mass spectrometry analysis together with a robust biochemical and biophysical characterization reveal the autophosphorylation kineti...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD053907 | Pride
Ccdc6 Phosphorylation Ret 
ProteomicsPhosphorylation of RXRa mediates the effect of JNK to suppress hepatic FGF21 expression and promote metabolic syndrome
The cJun NH2-terminal kinase (JNK) signaling pathway in the liver promotes systemic changes in metabolism by regulating PPARa-dependent expression of the hepatokine FGF21. Hepatocyte-specific gene ablation studies demonstrated that the Mapk9 gene (encodes JNK2) plays a key mechanistic role. Mutually...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-12 | PXD034183 | Pride
Metabolism Fgf21 Rxr Jnk Liver Phosphorylation 
ProteomicsNucleolar stress caused by arginine-rich peptides triggers a ribosomopathy and accelerates ageing in mice
Nucleolar stress (NS) kills cells by p53-dependent and independent manners. To investigate the mechanisms of p53-indendendent toxicity, we here used (PR)n arginine-rich peptides as inducers of NS, which are found in patients of some neurodegenerative diseases. Although these peptides generate NS, ho...
ORGANISM(S): Homo sapiens (Human) 
2024-03-11 | PXD043752 | Pride
Nucleolar stress Translation Ribosomopathy Dprs Mtor 
ProteomicsRank signaling drives basal cell-lineage infidelity leading to mammary tumorigenesis
Rank signaling regulates mammary gland development and epithelial cell differentiation. Rank receptor is expressed by mammary basal and luminal populations, but unlike that of luminal, the contribution of basal Rank signaling to mammary gland homeostasis remains poorly studied. Combining timely-regu...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-02 | PXD058134 | Pride
Rank signaling Phosphorylation Tumorogenesis 
ProteomicsDisruption of basal cell identity by Rank signaling drives mammary tumorigenesis
Rank signaling regulates mammary gland development and epithelial cell differentiation. Rank receptor is expressed by mammary basal and luminal populations, but, unlike luminal Rank, the contribution of basal Rank signaling to MG homeostasis remains poorly studied. We have combined timely regulated ...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-02 | PXD054102 | Pride
Breast cancer Basal cell Identity infidelity Rank Mouse Mammary gland 
ProteomicsThe RNA Binding Protein HNRNPK induces p53-dependent nucleolar stress driving ribosompathy
The nucleolus is the membraneless organelle in the nucleus mainly responsible for ribosome biogenesis. It is also an excellent stress sensor and any changes on its architecture or composition leads to nucleolar stress deriving in cell cycle arrest and interruption of ribosomal activity. However, nuc...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-04 | PXD046699 | Pride
Ribosomepathy Mefs Tmt Hnrnpk 
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