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TranscriptomicsHeart-muscle expression ratios in pig obtained by 454-sequencing, iTRAQ-based proteomics and cDNA microarrays
The recent development within high-throughput technologies for expression profiling has allowed for parallel analysis of transcriptomes and proteomes in biological systems such as comparative analysis of transcript and protein levels of tissue regulated genes. Until now, such studies of have only in...
ORGANISM(S): Sus scrofa 
2009-01-22 | E-GEOD-10122 | biostudies-arrayexpress
UnknownImproved proteome coverage by using iTRAQ labelling and peptide OFFGEL fractionation.
Not available
S-EPMC2572582 | biostudies-literature
UnknownOptimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots.
Not available
S-EPMC3442994 | biostudies-literature
ProteomicsiTRAQ-Based Quantitative Proteomic Comparison of 2D and 3D Adipocyte Cell Models Co-cultured with Macrophages Using Online 2D-nanoLC-ESI-MS/MS
The demand for novel three-dimensional (3D) cell culture models of adipose tissue has been increasing, and proteomic investigations are important for determining the underlying causes of obesity, type II diabetes, and metabolic disorders. In this study, we performed global quantitative proteomic pro...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-26 | PXD014437 | Pride
3d-culture Macrophages Adipocytes Proteomic quantification Itraq 
ProteomicsProteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology
Transplantation is currently the best treatment option for end-stage renal disease (ESRD) patients. However, acute rejection (AR) is the main source of failure in renal transplantation. The current gold standard for diagnosis of AR involves renal biopsy, but it is invasive, time consuming, costly an...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD015336 | Pride
Proteomics Acute rejection Serum Renal allograft Itraq 
ProteomicsMolecular markers of adaptive responses in Toxoplasma gondii to Pyrimethamine treatment
In this study, we employed bio-orthogonal non-canonical amino acid tagging (BONCAT) combined with iTRAQ-based quantitative proteomics to investigate the effects of pyrimethamine (PM) on newly synthesised proteins (NSPs) in Toxoplasma gondii. Parasites were treated with varying concentrations of PM, ...
ORGANISM(S): Toxoplasma gondii 
2025-05-07 | PXD054994 | Pride
Boncat Pyrimethamine Toxoplasma gondii Quantitative proteomics Itraq 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches which rely on complete labelling of primary amines. However, serines, tyrosi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-03-14 | PXD056723 | Pride
Phospho-peptides Tmt N-hydroxy succinimide Methylamine Nhs-ester Hydroxylamine 
ProteomicsiTRAQ experiment to identify proteins that changed before and after NMT1 was knocked down in both Bel-7402 and Bel-7404 cells
We performed proteomics isobaric tags for relative and absolute quantification (iTraq) to identify proteins that changed before and after NMT1 was knocked down in both Bel-7402 and Bel-7404 cells.
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD011812 | Pride
Itraq，liver cancer，nmt1 knockdown 
ProteomicsiTRAQ LLC-PK1 CnI - Sum of peak intensities outperforms peak area integration in iTRAQ protein expression measurement by LC-MS/MS using a TripleTOF 5600+ platform
In the field of quantitative proteomics, the Isobaric Tags for Relative & Absolute Quanti-tation (iTRAQ) technology has demonstrated efficacy for proteome monitoring despite its lack of a consensus for data handling. In this study, after peptide and protein identification, we com-pared the widesprea...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2019-08-19 | PXD007891 | Pride
Llc pk-1 Itraq Calcineurin inhibitors 
UnknownBONCAT-iTRAQ Labelling Reveals Molecular Markers of Adaptive Responses in <i>Toxoplasma gondii</i> to Pyrimethamine Treatment.
Not available
S-EPMC11510713 | biostudies-literature
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