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ProteomicsEssential role of translation factor eIF5a in cytokine and cell cycle regulation in primary mouse CD8 T lymphocyte
Translation of specific mRNAs is tightly regulated in primary T cells to ensure correct timing and precise delivery of protein syntheses in response to environmental cues. Translation factor eIF5a is known to be important for protein synthesis and is expected to regulate translation of certain diffi...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-11 | PXD021063 | Pride
Cytokine Lymphocyte Hypusine Proliferation Eif5a 
MetabolomicsHepatic PPARα function and lipid metabolic pathways are dysregulated in polymicrobial sepsis

Despite intensive research and constant medical progress, sepsis remains one of the most urgent unmet medical needs of today. Most studies have been focused on the inflammatory component of the disease, however, recent advances support the notion that sepsis is accompanied by extensive metabolic ...

2020-01-10 | MTBLS1386 | MetaboLights
UnknownA patient with van Maldergem syndrome with endocrine abnormalities, hypogonadotropic hypogonadism, and breast aplasia/hypoplasia.
Not available
S-EPMC5640965 | biostudies-literature
ProteomicsHyperSCP: Combining Isotopic and Isobaric Labeling for Higher Throughput Single-Cell Proteomics
Recent developments in mass spectrometry-based single-cell proteomics (SCP) have resulted in dramatically improved sensitivity, yet the relatively low measurement throughput remains a limitation. Isobaric and isotopic labeling methods have been separately applied to SCP to increase throughput throug...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD040455 | Pride
Multiplex Single-cell proteomics High-throughput 
UnknownαV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo.
Not available
S-EPMC8491627 | biostudies-literature
ProteomicsSCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56
Meiosis creates genetic diversity by recombination and segregation of chromosomes. The synaptonemal complex assembles during meiotic prophase I and assists faithful exchanges between homologous chromosomes, but how its assembly/disassembly is regulated remains to be understood. Here we report how ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2020-12-07 | PXD022755 | Pride
Drosophila 
MetabolomicsHypoxia Dependent Inhibition of Glioblastoma Cell Proliferation, Invasion, and Metabolism by the Choline-Kinase Inhibitor JAS239
Background: Elevated choline kinase alpha (ChoK) levels are observed in most solid tumors, including glioblastomas (GBM), and ChoK inhibitors have demonstrated limited efficacy in GBM models. Given that hypoxia is associated with resistance to GBM therapy, we hypothesized that tumor hypoxia c...
2024-06-18 | MTBLS6212 | MetaboLights
ProteomicsLow cell number proteomic analysis using in-cell protease digests reveals a robust signature for cell cycle state classification
Comprehensive proteome analysis of rare cell phenotypes remains a significant challenge. We report a method for low cell number mass spectrometry (MS)-based proteomics using protease digestion of mildly formaldehyde-fixed cells in cellulo, which we call the ‘in-cell digest’. We combined this with AM...
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD028117 | Pride
Tk6 In-cell digest Ampl Cell cycle Human 
MetabolomicsContribution of fungal microbiome to intestinal physiology, early-life immune development and mucosal inflammation in mice
The gut microbiome consists of a multi-kingdom microbial community. Whilst the role of bacteria as causal contributors governing host physiological development is well established, the role of fungi remains to be determined. Here, we use germ-free mice colonized with defined species of bacteria, fun...
2022-01-14 | MTBLS1679 | MetaboLights
ProteomicsAugmenting Immunotherapy Impact by Lowering Tumor TNF Cytotoxicity Threshold
Wild-type or TRAF2 mutated melanoma cells were treated with TNFalpha or co-cultured with activated T-cells for various short incubations (5min to 6 hours). Cells were harvested and analyzed using mass spectrometry for treatment induced changes at the proteome and phosphoproteome level.
ORGANISM(S): Homo sapiens (Human) 
2025-02-14 | PXD008995 | Pride
T cell Melanoma Phosphorylation Traf2 
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