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ProteomicsMouse skin technical replicate negative control
Multivariate regression modelling provides a statistically powerful means of quantifying the effects of a given treatment while compensating for sources of variation and noise, such as variability between human donors and the behaviour of different peptides during mass spectrometry. However, methods...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-12 | PXD012784 | Pride
Bayesenproteomics Mouse skin Negative control 
ProteomicsPNGase-F treated and untreated peptides from primary human mesenchymal stem cells for protein/PTM quant benchmarking
Multivariate regression modelling provides a statistically powerful means of quantifying the effects of a given treatment while compensating for sources of variation and noise, such as variability between human donors and the behaviour of different peptides during mass spectrometry. However, methods...
ORGANISM(S): Homo sapiens (Human) 
2019-03-12 | PXD012782 | Pride
Bayesenproteomics Pngase-f Mesenchymal stem cell 
ProteomicsSerial mixture of peptides from mouse skin and primary human mesenchymal stem cells for protein quant methods benchmarking
Multivariate regression modelling provides a statistically powerful means of quantifying the effects of a given treatment while compensating for sources of variation and noise, such as variability between human donors and the behaviour of different peptides during mass spectrometry. However, methods...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-03-12 | PXD012772 | Pride
Bayesenproteomics Msc Skin 
ProteomicsIntracellular proteome (SDS/Na-DoC) extract from EP/LP MSCs grown on soft/stiff hydrogels for 4 days
Tissues are maintained by homeostatic feedback mechanisms in which cells can respond to, but also modify, the chemical and mechanical properties of the surrounding extracellular matrix (ECM). Mechano-sensitive mesenchymal stem cells (MSCs) resident in the marrow niche experience a diverse mechanical...
ORGANISM(S): Homo sapiens (Human) 
2022-10-10 | PXD013240 | Pride
Mscs Senescence Mechanotransduction Ageing 
ProteomicsPrimary human mesenchymal stem cells subjected to 1 Hz cyclic tensile strain, with and without 24 hour recovery
Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD013287 | Pride
Nuclear decoupling Msc Cts 
ProteomicsPrimary Human MSCs subjected to 1 hr intense cyclic tensile strain with/without 24 hr recovery
Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012949 | Pride
Strain Msc Flexcell 
ProteomicsPrimary human MSCs treated with two different RNAi against SUN2
Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012948 | Pride
Sun2 Msc Rnai 
ProteomicsProteome changes to primary human MSCs subjected to high-intensity cyclic tensile strain (CTS) aligned to SUN2 overexpressing samples
Our current understanding of cellular mechano-signalling is based on static models, which do not replicate the dynamics of living tissues. Here, we examined the time-dependent response of primary human mesenchymal stem cells (hMSCs) to cyclic tensile strain (CTS). At low-intensity strain (1 hour, 4%...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012873 | Pride
Msc Cts 
ProteomicsDoxycycline-induced SUN2 overexpression in Y-201 MSC cell line
Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-24 | PXD012863 | Pride
Sun2 Msc Overexpression 
ProteomicsProteomics on formalin-fixed and stained laser-capure microdissected human lung sections
A broad range of tissues have been examined by microscopy following a process of formalin fixation, paraffin embedment, sectioning and staining. Haematoxylin and eosin (H&E) – which respectively stain nuclei blue and other cellular and stromal material pink – are routinely used for clinical diagnosi...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD014762 | Pride
Lung Human Laser-capture Dissection Spatial proteomics Fixed 
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