Sort   by:  
 Page size 
ProteomicsAnalysis of newly synthesized proteins more sensitive to Proteinase K using pulse SILAC and control sample for pulse SILAC experiment in which yeast cells were heat shock for 15 min and supernatant fractions analyzed (Experiment 1)
Accurate and efficient folding of nascent protein sequences into their native state requires support from the protein homeostasis network. Herein we probed which newly translated proteins are thermo-sensitive to infer which polypeptides require more time to fold within the proteome. Specifically, we...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-10 | PXD028934 | Pride
Protein homeostasis Yeast Pulse silac Limited proteolysis 
ProteomicsDeubiquitinase activity is required for the degradation of misfolded cytosolic proteins upon heat-stress
Elimination of misfolded proteins is crucial for proteostasis and to prevent proteinopathies. Nedd4/Rsp5 emerged as a major E3 ligase involved in multiple quality control pathways that target misfolded plasma membrane proteins, aggregated polypeptides, and cytosolic heat-induced misfolded proteins f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-03-01 | PXD004747 | Pride
Deubiquitinase Cytosolic protein quality control Heat stress 
ProteomicsMining Yeast Diversity Unveils Novel Targets for Improved Heterologous Laccase Production in Saccharomyces cerevisiae
Analysis of proteins from total cell lysates of 21 different Saccharomyces cerevisiae strains while expressing Trametes trogii laccase.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
MSV000095757 | MassIVE
ProteomicsRole of 14-3-3ζ in the adipogenesis program and the differentiation of healthy adipose tissue
Obesity is an independent risk factor for diabetes and cardiovascular disease. Before effective anti-obesity therapies can be developed, understanding of what governs the production of healthy versus unhealthy fat tissue is required, as not all types confer equal risk. Adipogenesis requires the prec...
ORGANISM(S): Mus musculus (Mouse) 
2022-03-01 | PXD001215 | Pride
14-3-3 Adipogenesis Adipocytes Differentiation Molecular adaptors 
ProteomicsNewly translated protein misfolding after heat shock and translation during heat shock
Correct and efficient folding of nascent proteins to their native state requires support from the protein homeostasis network. We set to examine which newly translated proteins are less thermostable to infer which polypeptides require more time to fold. More specifically, we sought to determine whic...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-10 | PXD024336 | Pride
Proteomics Protein folding Protein aggregation Proteostasis Heat stress 
ProteomicsYeast Heatshock SILAC LC-MSMS
Yeast cells were incubated at 25˚C, unless otherwise stated. For SILAC, cells were grown in light labeled (0.03mg/ml Lys0, 0.02mg/ml Arg0) or heavy labeled (0.03mg/ml Lys4, 0.02mg/ml Arg6) amino acid in synthetic defined medium with 2% dextrose for at least 7 generations. heat shock was performed i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-06-12 | PXD012631 | Pride
Stress granule Yeast Heatshock 
ProteomicsProteomic analysis of [PSI+] yeast strain
The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI+] prion state leads to more translational read-through. Although extensive studies have been done on [PSI+], changes at the proteomic level have not been performed exhaustively. We therefore us...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-10-24 | PXD007168 | Pride
Yeast prion Proteomics Sup35 Mass spectrometry Silac Aneuploidy Amyloids 
ProteomicsCo-IP experiment with Pdr3 under mitochondrial import impairment
Co-IP experiment from lysates derived from V5-tagged Pdr3 cells analyzed on a Exploris 480
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-10-16 | PXD066688 | Pride
Mitochondria Mts Yeast Immunoprecipitation V5 
Sort   by:  
 Page size