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ProteomicsHistone variant H2A.Z deposition and acetylation directs the canonical Notch signaling response
The study deals with the elucidation of potential interaction partners of the intracellular domain of the transmembrane receptor Notch1, termed NICD. The NICD is released from the cytoplasmic tail of the Notch receptor by gamma-secretase treatment and translocated as a transcription factor to the nu...
ORGANISM(S): Mus musculus (Mouse) 
2018-06-25 | PXD008747 | Pride
Ap-ms Notch P400 Orbitrap ms Histone variant Maxquant Tip60 Crispr H2a.z 
ProteomicsComplete loss of H3K9 methylation dissolves mouse heterochromatin organization
Histone H3 lysine 9 (H3K9) methylation is a central epigenetic modification that defines heterochromatin from unicellular to multicellular organisms. In mammalian cells, H3K9 methylation can be catalyzed by at least six distinct SET domain enzymes: Suv39h1/Suv39h2, Eset1/Eset2 and G9a/Glp. We used m...
ORGANISM(S): Mus musculus (Mouse) 
2021-05-27 | PXD018175 | Pride
Repeat silencing Heterochromatin organization Histone methyltransferases Proteomic quantification of h3k9 methylation Genome stability 
ProteomicsDictyostelium DIA LFQ proteomics of vegetative and developmental growth
Dictyostelium discoideum behavior depends on nutrients. When sufficient food is present these amoebae exist in a unicellular state (vegetative growth), but upon starvation they aggregate into a multicellular organism (developmental growth). For proteomics 30 minutes and eight hour cultures of D. dis...
ORGANISM(S): Dictyostelium discoideum (Slime mold) 
2021-03-23 | PXD023404 | Pride
Dictyostelium discoideum  non-starved (vegetative)  starved (developmental)  dia hyper reaction monitoring  spectronaut  sulfur metabolism  reactive oxygen species 
ProteomicsMitochondrial Membrane Potential Regulates Nuclear Gene Expression in Macrophages Exposed to PGE2
Metabolic engagement is intrinsic to immune cell function. Prostaglandin E2 (PGE2) has been shown to modulate macrophage activation, yet how PGE2 might affect metabolism is unclear. Here we show that PGE2 causes mitochondrial membrane potential (Δψm) to dissipate in interleukin-4 activated macrophag...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-08 | PXD010997 | Pride
Macrophage Prostaglandin e2 Camp Got1 Malate-aspartate shuttle Immunometabolism Mitochondrial membrane potential Etv1 Alternative activation Il-4 
ProteomicsHeat shock dependent proteome changes in HEK293 chromatin proteome
Identification of proteins that associate with chromatin (fraction) upon heat shock by SILAC-based quantitative proteomics
ORGANISM(S): Homo sapiens (Human) 
2022-04-07 | PXD012077 | Pride
Hek293 cells Silac Heat shock response Gelc-ms Chromatin 
ProteomicsKansl2 dependent acetylome study in primary Kansl2 (fl/fl) murine embryonic fibroblasts
As one of the most important post-translational protein modifications, lysine acetylation is catalyzed by lysine acetyltransferases (KATs), which catalyze the covalent attachment of an acetyl group to the N epsilon–residue of lysine. The histone acetyltransferase MOF, which represents the main histo...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-05 | PXD038521 | Pride
Kat8 regulatory nsl complex subunit 2  mef (murine embryonic fibroblast)  primary inducible kansl2 knock-out  cre-ert2  anti acetyl-lysine antibody  ptm enrichment 
ProteomicsLKB1/STK11-sensing of mitochondrial function controls Th17 cell responses
T helper 17 (Th17) cells are a distinct subset of CD4+ T cells necessary for maintaining gut homeostasis and have prominent roles in autoimmunity and inflammation. Th17 cells have unique metabolic features, including a stem cell-like signature and reliance on mitochondrial respiratory chain function...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD036162 | Pride
Dia Spectronaut Th17 t cells Serine/threonine kinase liver associated kinase b1 (lkb1/stk11). Opa1 knock out th17 cells 
ProteomicsA Polyamine-mediated Posttranslational Modification Required For Macrophage Tissue Residency – Differential proteome analysis of DHPS deficient macrophages
Tissue resident macrophages (MTRs) regulate tissue repair and homeostasis by clearing cell debris, and are thought to form the first line of defense against pathogens. MTRs form early in life and self-renew locally. However, during tissue damage or disease, bone-marrow derived monocytes enter tissue...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-23 | PXD054670 | Pride
Eif5a Dia Spectronaut Dda spectral library Tissue resident macrophage proteomics Maxquant Label-free quantification Deoxyhypusine synthase (dhps) Hypusine 
ProteomicsPolyamines and eIF5A hypusination modulate mitochondrial respiration and macrophage activation
How cells adapt metabolism to meet demands is an active area of interest across biology. Amongst a broad range of functions, the polyamine spermidine is needed to hypusinate the translation factor eukaryotic initiation factor 5A (eIF5A). We show here that hypusinated eIF5A (eIF5AH) promotes the effi...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-24 | PXD013443 | Pride
Proteomics Macrophage Polyamine Eif5a 
ProteomicsStress granules proteome composition
Analysis of stress granules in HeLa cells, after exposure to different stimuli. Granules were collected by a newly devised enrichment method, described in manuscript. Proteomics sample preparation of granules was carried out using the Single-pot, solid phase-enhanced sample preparation, SP3 method (...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-11-02 | MSV000090634 | MassIVE
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