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Quantitative proteomic analysis of human post-mortem substantia nigra from patients with Parkinson’s disease (n=3) versus controls (n=3) based on sixplex TMT (TMT6) isobaric labeling which allowed protein simultaneous identification and quantification. Samples were digested and pooled after TMT6 lab...
ORGANISM(S): Homo sapiens (Human) 
2014-01-28 | PXD000427 | Pride
The associated files are mass spec data from native nuclear extract from broccoli florets (Brassica oleracea var. italica)fractionated on an Agilent 3100 OFFGEL isoelectric focusing apparatus.
ORGANISM(S): Brassica oleracea var. italica 
2019-10-28 | PXD013281 | Pride
We used LC-MS/MS based proteomics to identify changes in protein expression in the the female and male mouse hippocampus at 1, 2, and 4 months of age. This proteomics dataset was correlated with an accompanying mRNA dataset to identify genes that show mRNA changes in a different direction that their...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-21 | PXD004496 | Pride
For the analysis of chromosome 11-centric human proteome, we analyzed the human brain hippocampal proteome. The samples from brain tissues were extensively off-gel fractionated by 12 fractions and analyzed by a high resolution mass spectrometer, Orbitrap XL ETD with collision induced dissociation...
ORGANISM(S): Homo sapiens (Human) 
2016-10-10 | PXD000395 | Pride
Phosphoproteome analysis of HL-1 cardiomyocytes carrying AKT1 or AKT2 isoform specific knock down, respectively. Six biological replicates were analysed. Cells were grown until reaching 90 % confluency, starved for 50 minutes followed by stimulation with 200 nM insulin for 10 minutes. In solution st...
ORGANISM(S): Mus musculus (Mouse) 
2014-08-28 | PXD000268 | Pride
Proteins in milk have been studied for decades and proteomics, peptidomics, and glycoproteomics are the main approaches previously deployed to decipher the proteome of human milk. In the present work, we aimed at implementing a highly automated pipeline for the proteomic analysis of human milk with ...
ORGANISM(S): Homo sapiens (Human) 
2021-08-01 | PXD021974 | Pride
Wild type (wt) MCF7 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the a...
ORGANISM(S): Homo sapiens (Human) 
2016-06-01 | PXD004085 | Pride
Wild type (wt) SUM44 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the ...
ORGANISM(S): Homo sapiens (Human) 
2017-12-11 | PXD004807 | Pride
Proteomic analysis of regulated proteins in female mouse liver extracts derived from 1 week and 8 weeks of hURI expression.
ORGANISM(S): Mus musculus (Mouse) 
2016-07-05 | PXD000296 | Pride
To get further insights in the dimethyl sulfide metabolism of Hyphomicrobium denitrificans, a LC-MS proteomic approach was used. The total protein of three biological replicates of H. denitrificans grown on dimethyl sulfide was compared with three biological replicates grown on dimethylamine. This a...
ORGANISM(S): Hyphomicrobium denitrificans ATCC 51888 
2018-06-14 | PXD009030 | Pride
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