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Proteomicsyeast_cell_lysate
yeast_quality_control
ORGANISM(S): Saccharomyces Cerevisiae 
2006-12-31 | PAe000324 | PeptideAtlas
ProteomicsA dataset describing a suite of novel antibody reagents for the RAS signaling network
RAS genes are frequently mutated in cancer and have for decades eluded effective therapeutic attack. The National Cancer Institute’s RAS Initiative has a focus on understanding pathways and discovering therapies for RAS-driven cancers. Part of these efforts is the generation of novel reagents to ena...
ORGANISM(S): Homo sapiens (Human) 
2019-07-18 | PXD012130 | Pride
Ap-ms Sds-page In-gel digestion Ras Monoclonal antibody generation Lc-ms/ms 
ProteomicsMultiomic analysis implicates OXPHOS/fatty acid oxidation as a potential druggable target in platinum-resistant high grade serous ovarian cancer
In the United States, high grade serous ovarian cancer (HGSOC) is the most lethal gynecologic malignancy and tumor response to platinum-based chemotherapy is a major determinant of clinical outcome. Although recent efforts have dramatically improved the median survival of advanced ovarian cancer, th...
ORGANISM(S): Homo sapiens (Human) 
2021-12-22 | PXD020764 | Pride
Human Shotgun proteomics Global Tmt Acetylation Ubiquitinylation Fractionation Ovarian cancer Phosphorylation Pdx Cell line 
ProteomicsTargeted mass spectrometry enables quantification of novel pharmacodynamic biomarkers of ATM kinase inhibitors
ATM is a serine/threonine protein kinase that is responsible for initiation of DNA repair of double-stranded breaks and is a therapeutic target in cancer. A lack of analytically robust and multiplexed assays has hampered mechanistic studies of action and determination of optimal, robust pharmacodyna...
ORGANISM(S): Homo sapiens (Human) 
2021-07-29 | PXD026103 | Pride
Human Lymphoblast Atm Phosphorylation 
ProteomicsGlobal and phosphoproteomic profiling of the DNA damage response in yeast by SILAC labeling and LC-MS/MS
Wild type yeast cells were metabolically labeled in SILAC medium. The heavy (H)-SILAC-labeled cells were then continuously exposed to 0.01% MMS to induce replication stress, and the light (L)-SILAC-labeled cells were mock-exposed. After 3 hours, heavy and light cells were harvested and lysed. To ens...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-04-04 | PXD002344 | Pride
Dna damage checkpoint Mass spectrometry Genetic interaction Telomere Methylmethanesulfonate Dna damage response Phosphorylation Homologous recombination 
ProteomicsPhosphoproteomic profiling of the DNA damage response in MCF10A and human PBMC cells by SILAC labeling and 1D and 2D-LC-MS/MS
The DNA-damage response (DDR) is a critical network for maintaining genomic integrity, and mutations in the DDR are among the most frequently identified in tumors. Phosphorylation is a key signaling event in the DDR network. We sought to design high throughput, mass spectrometry based assays to moni...
ORGANISM(S): Homo sapiens (Human) 
2015-12-01 | PXD002363 | Pride
Human Pbmc Silac Ir Mms Mcf10a Lc-ms/ms 
ProteomicsHighly multiplexed quantification by targeted mass spectrometry to triage protein candidate biomarkers in plasma
Despite improvements in capabilities of proteomics technologies, the introduction of new plasma-based protein biomarkers for clinical use remains low. One reason is the cumbersome requirement to test thousands of protein candidates in follow-up quantitative verification studies. We sought to evaluat...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-06-29 | PXD028306 | Pride
Human Breast cancer Plasma Is-prm Protein Lc-ms/ms 
ProteomicsPreserving the phosphoproteome of clinical biopsies using a quick freeze collection device
The current gold standard for collecting and processing cancer biopsies is to flash freeze tumor samples in liquid nitrogen. However, in many clinical settings liquid nitrogen is not readily available, and neither the personnel nor the infrastructure is generally available to rapidly process the tum...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-18 | PXD029844 | Pride
Preanalytics Imac Tmt Fractionation Tissue processing Phosphorylation Pdx Diagnostics Lc-ms/ms 
ProteomicsRNA and phosphoprotein profiles of TP53- and PTEN-knockouts in MCF10A at baseline and responding to DNA damage
A wealth of proteogenomic data has been generated using cancer samples to deepen our understanding of the mechanisms of cancer and how biological networks are altered in association with somatic mutation of tumor suppressor genes, such as TP53 and PTEN. To generate functional signatures of TP53 or P...
ORGANISM(S): Homo sapiens (Human) 
2023-11-20 | PXD028494 | Pride
Knockout Imac Phosphoproteomics Silac Mms Mcf10a Lc-ms/ms 
Proteomics1D LC-MS/MS proteomic profiling of MCF10A, HeLa, Hep G2, and HEK293 cells using peptide-based immunoaffinity enrichment and 105 commercially available monoclonal antibodies to proteins associated with the DNA damage response (DDR) network
Immunoaffinity enrichment of peptides coupled to multiple reaction monitoring-mass spectrometry (immuno-MRM) enables highly specific, sensitive, and precise quantification of peptides and post-translational modifications. Major obstacles to developing a large number of immuno-MRM assays are the poo...
ORGANISM(S): Homo sapiens (Human) 
2016-04-13 | PXD003370 | Pride
Human Monoclonal antibodies Hela Ir Hek293 Mcf10a Hep g2 Lc-ms/ms 
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