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ProteomicsArabidopsis thaliana DOG1 pull-downs from seed tissues
Functionally complemented dog1-1 mutant with a YFP-DOG1 fusion protein under the control of its native promoter were used as starting material for in vivo pull-down of YFP: DOG1 and its interacting proteins from seed tissues in native conditions. DOG1 interacting proteins were identified by mass spe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-07-07 | PXD006347 | Pride
Arabidopsis thaliana Dormancy Dog1 Seed Germination 
ProteomicsArabidopsis thaliana anti-all-WRKY pull-downs from whole seedlings
The experiments were performed to monitor dynamics in WRKY transcription factor abundancies upon treatment with flg22, a peptide derived from the bacterial flagella. Mock-treated or WT seedlings treated for 2 h with flg22 were used to prepare crude nuclear lysates. Then pull downs were performed wit...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-07-30 | PXD008971 | Pride
Wrky Arabidopsis Pull-down Flg22 
ProteomicsControl pull-downs testing the effect of histone native chemical ligation on protein binding to di-nucleosomes
Here we examined whether histones H3 and H4 produced using native chemical ligation reaction affect protein binding to di-nucleosomes. To test this we performed a set of affinity purification pull-downs using di-nucleosomes containing the following histones H3 and H4: 1. ligated unmodified histone H...
ORGANISM(S): Homo sapiens (Human) 
2023-12-10 | PXD042630 | Pride
Nucleosome pull-down Native chemical ligation 
ProteomicsAnalysis of ZNF827 Halotag pulldown for a manuscript titled ‘ZNF827 is a single-stranded DNA binding protein that regulates the ATR-CHK1 DNA damage response pathway’
Mass spectrometry analysis of ZNF827 Halotag pulldown ZNF827 pull-downs were performed in HEK 293T cells overexpressing HaloTag ZNF827 or empty vector control using the HaloTag® Protein Pull-Down and Labelling System (Promega) according to the manufacturer’s protocol. Pull-downs were subjected mass ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-23 | PXD050191 | Pride
Atr Ssdna Znf827 Dna damage response 
ProteomicsDPP8/9 processing of human AK2 unmasks an IAP binding motif
AK2 is n-terminally processed by methionine aminopeptidases, N-terminal acetylation and DDP8/9 to become an active protein which is then sorted in the mitochondrial intermembrane space. We used peptide pull-downs of free and acetylated N-terminal peptides of AK2 from the different processing steps t...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD047178 | Pride
Ddp8/9 cleavage Peptide pull-downs Mass spectrometry N-terminal processing N-terminal acetlyation 
ProteomicsGPIF constrains Polycomb Repressive Complex 2 activity in germ cells
The project aimed at identifying the cofactors regulating Polycomb complex PRC2 enzymatic activity in gonads. We used a knock-in mouse model where the enzymatic subunit of PRC2 (either EZH2 or EZH1) is tagged (Flag-tag) to purify PRC2 from mouse adult testis. This experiment revealed the existence o...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-07-22 | PXD012354 | Pride
Histone Chromatin Methylation Polycomb. 
ProteomicsThe interactome of Zbtb2 in mouse embryonic stem cells
Zbtb2 is a transcription factor that plays a role in mouse embryonic stem cell (mESC) differentiation. We pulled-down endogenously tagged Zbtb2-FLAG in mESCs and found a complex interactome. We characterized the Zbtb2 complex further by co-pull-downs of overexpressed Zbtb2-AVI, after mutation of ind...
ORGANISM(S): Mus musculus (Mouse) 
2021-07-14 | PXD025520 | Pride
Zbtb2 interactome Development Mouse embryonic stem cells 
ProteomicsSelective Arylation of Selenocysteine of Thioredoxin Reductase 1 by an Organogold Compound: Expanding the Tool-Box of Metal-Templated Reactions in Cancer Cells – pull-down
Cyclometalated gold(III) complexes have been reported to template C-S cross-coupling reactions in a biological environment and acting as modifies of cysteine residues. To broaden the scope of organogold complexes for covalent protein post-translational modification in cancer cells, an oxime-containi...
ORGANISM(S): Homo sapiens (Human) 
2025-04-22 | PXD044971 | Pride
Arylation ∙ cancer ∙ chemoproteomics ∙ gold ∙ metals in medicine ∙ selenoprotein ∙ thioredoxin reductase 1 
ProteomicsMass spectrometry analysis of estrogen receptor α -interacting proteins.
Quantitative mass spectrometry analysis using tandem mass tags (TMT) labelling of estrogen receptor α (ERα) pull downs. Co-immunoprecipitated proteins using a anti Erα antibody were compared quantitatively with material recovered in a mock to identify ERα-interacting proteins.
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD025018 | Pride
Estrogen receptor Mrna binding Tmt Immunoprecipitation 
ProteomicsProteomics analysis of ALK signaling in neuroblastoma cells
We used mass spectrometry-based proteomics to unravel anaplastic lymphoma kinase (ALK) signaling in the ALK and MYCN amplified neuroblastoma cell line, NB1. We specifically measured the ALK interactome, phosphoproteome, phosphotyrosine interactome and proteome as outlined below. ALK Interactome and ...
ORGANISM(S): Homo sapiens (Human) 
2018-11-21 | PXD006404 | Pride
Lc-msms Neuroblastoma Alk signaling Human 
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