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ProteomicsPCT-assisted mass spectrometric quantification of gingival tissue reveals dynamics of protein synthesis during initiation and progression of inflammatory disease
Understanding the progression of periodontal destruction is at the forefront of periodontal research. Therefore, we aimed to capture the dynamics of gingival tissue proteome during initiation and progression of an experimental periodontitis developed by placing silk ligatures on the second molars of...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-09 | PXD014608 | Pride
Experimental periodontitis Gingival tissue Pressure cycling technology Label-free quantitation Proteome Gingival inflammation Periodontitis Pct 
ProteomicsPCP39: prostate cancer proteome for 39 patients by PCT-SWATH
here we processed 105 biopsy-level tissues from 39 prostate cancer patients using pressure cycling technology (PCT) and SWATH-MS
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD004589 | Pride
Prostate cancer  pct  swath 
ProteomicsAtopic dermatitis epidermis PCT-DIA
Atopic Dermatitis (AD) is the most common inflammatory skin disease and characterized by a deficient epidermal barrier and cutaneous inflammation. Genetic studies suggest a key role of keratinocytes in AD pathogenesis, but the alterations in the proteome that occur in the entire epidermis have not b...
ORGANISM(S): Homo sapiens (Human) 
2022-10-04 | PXD025431 | Pride
Pressure cycling technology Atopic dermatitis Data-independent acquisition Epidermis 
ProteomicsProteome mapping of human gingival tissue in health and disease
Gingival tissue proteome is at the forefront of the periodontal research, yet have been hampered by lack of sufficient tissue-extraction methods. Using an emerging technology for tissue homogenization, pressure cycling technology (PCT), we characterize the gingival tissue proteomic profiles of both ...
ORGANISM(S): Homo sapiens (Human) 
2020-09-25 | PXD018029 | Pride
Pressure cycling technology Gingival tissue proteome Chronic periodontitis 
ProteomicsABLE-SWATH - A Case Study and Methodology for OpenSWATH Parameter Optimization Using the ProCan90 Data Set and 45,810 Computational Analysis Runs
Proteomic methods typically involve lengthy, multi-step sample preparation protocols (14-16 hrs), especially for the lysis and digestion of solid tissue samples or cells. We developed a streamlined proteomic sample preparation protocol termed Accelerated Barocycler Lysis and Extraction (ABLE), that...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2019-01-21 | PXD010688 | Pride
Urea Sample preparation Clinical proteomics Swath Barocycler Sdc Pct 
ProteomicsHeat ‘n Beat: A universal high-throughput end-to-end proteomics sample processing platform in under an hour
Clinical proteomics on solid tissue samples from human tumour biopsies present multiple challenges. These include limited sample availability, reducing endogenous protease activity and challenges with efficiently solubilising and digesting proteins for high throughput mass spectrometry while achievi...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2025-03-26 | PXD045405 | Pride
Sample preperation Proteomics High-throughput Heat n beat Universal Mass spec 
ProteomicsQuantification of brain, muscle, and liver using large SWATH-spectral library
Sequential window acquisition of all theoretical mass spectra (SWATH-MS) requires a spectral library to extract quantitative measurements from the mass spectrometry data acquired in data-independent acquisition mode (DIA). Large combined spectral libraries containing SWATH assays have been generated...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2018-12-18 | PXD010869 | Pride
Brain Liver Zebrafish Muscle 
ProteomicsHuman brain LC-MS/MS - Brain Citrullination Patterns and T Cell Reactivity of Cerebrospinal Fluid-Derived CD4+ T Cells in Multiple Sclerosis
Citrullination as a post-translational modification has been described in a couple of autoimmune diseases like rheumatoid arthritis (RA) and multiples sclerosis (MS). We investigated citrullination brain tissue from MS patients and healthy individuals (HD). The myelin sheath proteins have been the f...
ORGANISM(S): Homo sapiens (Human) 
2019-04-10 | PXD008344 | Pride
Pressure cycle technology (pct) Post-translational modification (mbp) Mass spectrometry Human post mortem brain tissue Hydrophilic interaction liquid chromatography hilic Myelin basic protein Citrullination Multiple sclerosis (ms) Neurodegeneration 
ProteomicsTMT-based proteomics of BMDMs following virus infection
To identify potential regulators involved in virus-triggered innate immune response, we used tandem mass tagging (TMT)-based shotgun proteomic analysis of murine bone marrow-derived macrophages (BMDMs) following sendai virus (SeV) infection. This approach facilitated the identification and relative ...
ORGANISM(S): Mus musculus (Mouse) 
2025-04-03 | PXD051098 | Pride
Mus bmdms 
ProteomicsLabel-free quantitative SWATH-MS proteomic analysis of adult myocardial slices in vitro after biomimetic electromechanical stimulation
Conventional in vitro cell cultures are relatively available and have been widely used in explaining many mechanisms, but their simple construction is not directly applied to the in vivo environment. A possible solution to overcome this problem is special in vitro model maintained with cardiac slice...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-08-25 | PXD034891 | Pride
Rat Heart Myocardial slices Swath-ms 
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