Sort   by:  
 Page size 
UnknownOff-the-shelf proximity biotinylation for interaction proteomics.
Not available
S-EPMC8373943 | biostudies-literature
ProteomicsControl of TurboID-dependent biotinylation intensity in proximity ligation screens
Proximity biotinylation screens are a widely used strategy for the unbiased identification of interacting or vicinal proteins. The latest generation biotin ligase TurboID has broadened the range of potential applications, as this ligase promotes an intense and faster biotinylation, even in subcellul...
ORGANISM(S): Homo sapiens (Human) 
2023-03-29 | PXD040302 | Pride
Biotinylation Proximity ligation screens Protein-protein interactions Proteomics 
Proteomics‘Off the shelf’ proximity biotinylation for interaction proteomics
Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins of interest usin...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-07-12 | PXD025012 | Pride
Flywch1 Proximity labelling Turboid Interactome Protein a 
UnknownTopological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation.
Not available
S-EPMC7401799 | biostudies-literature
UnknownIdentifying Protein-Protein Interactions by Proximity Biotinylation with AirID and splitAirID.
Not available
S-EPMC10031415 | biostudies-literature
ProteomicsProteomic analysis of the NF2/Merlin Interactome by Proximity Biotinylation
Neurofibromatosis type 2 is an inherited neoplastic disease consisting of schwannomas, meningiomas, and ependymomas that is caused by inactivation of the tumor suppressor gene NF2. The NF2 gene product, merlin, has no intrinsic catalytic activity; its tumor suppressor function is mediated through t...
ORGANISM(S): Mus musculus (Mouse) 
2019-04-25 | PXD012017 | Pride
Proximity biotinylation Schwann cell. Nf2 Neurofibromatosis type 2 Merlin Interactome Bioib 
ProteomicsDevelopment of a method for quantitative analysis of in vivo interactions of pluripotency transcription factors Sox2, Oct4, Nanog by mass spectrometry
Protein-protein proximity of core pluripotency transcription factors plays an important role during cell reprogramming. Pluripotent embryonic stem (ES) cell identity is controlled by a trio of transcription factors: Sox2, Oct4, and Nanog. These proteins often bind to closely localized genomic sites....
ORGANISM(S): Homo sapiens (Human) 
2020-01-21 | PXD015756 | Pride
Mass spectrometry Oct4 Proximity utilizing biotinylation Transcription factors Sox2 Pluripotency 
ProteomicsOff the shelf proximity biotinylation using ProtA-TurboID
Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or CRISPR-based tagging, thus prohibiting such assays in (primary) cell types that a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-17 | PXD030963 | Pride
Cell lines Centromeres Biotinylation Histone ptms Protein-protein interactions 
ProteomicsProximity-dependent biotinylation by TurboID to identify protein-protein interaction networks in yeast
The use of proximity-dependent biotinylation assays coupled to mass spectrometry (PDB-MS) has changed the field of protein-protein interactions (PPI) studies. Yet, despite the recurrent and successful use of BioID-based PPI screening in mammalian cells, the implementation of PDB-MS in yeast has not ...
ORGANISM(S): Schizosaccharomyces pombe 927 
2019-05-16 | PXD013183 | Pride
Proximity-dependent biotinylation  turboid  yeast  s. pombe  rmt3 Rna exosome 
ProteomicsSuper-resolution proximity labeling method with enhanced direct identification of biotinylation sites
Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein-protein interactions. Although the conventional approach of enriching biotinylated proteins is widely implemented, in-depth identification of specific biotin...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD047979 | Pride
Biotinylation site Apex2 Turboid Lc-ms Membrane protein topology Bioid Proximity labeling 
Sort   by:  
 Page size