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ProteomicsAnalysis of extracellular matrix proteins from 3D cultures of primary prostate fibroblasts, DU145 cells and mixed cells
Composition and organization of extracellular matrix (ECM) in tumor stroma are important regulators of cancer cell behavior. Therefore, ECM produced by cancer associated fibroblasts is often used in in vitro experiments. Here, a proteome wide analysis of ECM proteins in 3D culture is analysed.
ORGANISM(S): Homo sapiens (Human) 
2018-03-13 | PXD006563 | Pride
3d culture Prostate cancer Fibroblast Extracellular matrix 
ProteomicsAnalysis of citrullination of extracellular matrix proteins in spheroid cultures of fibroblasts and cancer cells
Eight different human cancer cell lines were cocultured with human cancer associated fibroblasts (CAF) as spheroid cultures. In another setup UT-SCC-7 cutaneous squamous cell carcinoma cells, together with human skin fibroblasts (SFB) and UT-SCC-2 cells together with gingival fibroblasts (GFB) were ...
ORGANISM(S): Homo sapiens (Human) 
2022-11-21 | PXD033555 | Pride
Citrullination Cancer Inflammation Spheroid 
ProteomicsComparison of extracellular matrix in prostate tissue samples and in cultures of primary fibroblasts from prostate cancer patients
xtracellular matrix from prostate tissue samples and from cultures of primary fibroblasts of the same samples from individuals was analysed by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2018-03-13 | PXD006562 | Pride
Prostate tissue Extracellular matrix Primary fibroblasts 
ProteomicsProteomic analysis of 3D cultures of fibroblasts and Ras-transformed HaCaT keratinocytes
HaCaT, and its three retrovirally c-Ha-ras-transformed subclones, A5, II-4 and RT3 (abbreviated as ras-HaCaTs in the text) that exhibit benign, invasive and malignant phenotypes, respectively, were cultured as 3D monocultures and co-cultures with fibroblasts. After hypotonic lysis of the cells, the ...
ORGANISM(S): Homo sapiens (Human) 
2019-09-10 | PXD013113 | Pride
Fibroblast keratinocyte Hacat Hras 
ProteomicsProline hydroxylation of collagens in prolyl 4-hydroxylase mutant mice
Collagen from the skin of wild type and prolyl 4-hydroxylase mutant mice was extracted and proline hydroxylation of the collagen-derived peptides were analyzed by LC-MS/MS.
ORGANISM(S): Mus musculus (Mouse) 
2018-04-10 | PXD008802 | Pride
Mouse Collagen prolyl 4-hydroxylase 
ProteomicsExtracellular citrullination in synovial fluid
Citrullination of the proteins found in synovial fluid patient samples
ORGANISM(S): Homo sapiens (Human) 
2017-08-15 | PXD004017 | Pride
Lc-msms Synovial fluid Fibronectin Human 
ProteomicsEffect of TPA on focal adhesion composition
Effect of a tumor promoter TPA on collagen IV based focal adhesions from CHO cells expressing human integrin alpha1 with activating mutation E317A
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2016-07-11 | PXD003153 | Pride
Focal adhesions Cho cells Lc-ms/ms 
TranscriptomicsTranscriptomic analysis of CT16 (PAGE5) function in A2058 and WM-266-4 melanoma cells
This SuperSeries is composed of the following subset Series: GSE31350: Transcriptomic analysis of the effect of CT16 (PAGE5) expression in WM-266-4 melanoma cells GSE31351: Transcriptomic analysis of the effect of CT16 (PAGE5) silencing by RNAi in A2058 melanoma cells Refer to individual Series
ORGANISM(S): Homo sapiens 
2012-08-09 | E-GEOD-31352 | biostudies-arrayexpress
ProteomicsCitrullination of latency-associated peptide of TGF beta1
Enzymatic citrullination of latency-associated peptide of TGF beta1 by PAD2
ORGANISM(S): Homo sapiens (Human) 
2016-03-14 | PXD003132 | Pride
Tgfb1 Lc-ms/ms 
ProteomicsComparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine
Extracellular vesicles (EV) in body fluids are extensively studied as potential biomarkers for numerous diseases. Major impediments of EV-based biomarker discovery include the manual labor, and the specificity and reproducibility of EV sample preparation. To tackle this, we present an automated liqu...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD032394 | Pride
Plasma Urine Extracellular vesicle Automatic liquid handling 
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