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ProteomicsIdentification of AURORA A interaction partners
The oncogenic kinase AURORA A is essential for mitotic progression, and its catalytic inhibition arrests cells at the G2/M-transition. Unexpectedly, partial degradation of AURORA A by PROTACs (proteolysis targeting chimeras) induces profound S-phase defects, revealing a non-catalytic scaffolding fun...
ORGANISM(S): Homo sapiens (Human) 
2026-06-03 | PXD069843 | Pride
Coip Interactome 
ProteomicsComplex regulation of Gephyrin splicing is a determinant of inhibitory postsynaptic diversity
Gephyrin (GPHN) regulates the clustering of postsynaptic components at inhibitory synapses and is involved in pathophysiology of neuropsychiatric disorders. Here, we uncover an extensive diversity of GPHN transcripts that are tightly controlled by splicing during mouse and human brain development. P...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-28 | PXD033751 | Pride
Splice variants Gephyrin Inhibitory synapses 
ProteomicsThe 3’-UTR of MYC couples RNA polymerase II function to ribonucleotide levels
Deregulated expression of MYC enhances glutamine utilization and renders cell survival dependent on glutamine, inducing “glutamine addiction”. Surprisingly, colon cancer cells that express high levels of MYC due to WNT pathway mutations, are not glutamine-addicted but undergo a reversible cell cycle...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD005829 | Pride
Myc Mrna-protein interaction Glutamine Nanolc-ms/ms 
ProteomicsThe translational landscape of HIV-1 infected cells reveals novel gene regulatory principles
Human Immunodeficiency Virus-1 (HIV-1) uses a number of strategies to modulate viral and host gene expression during its lifecycle. To characterize the transcriptional and translational landscape of HIV-1 infected cells, we used a combination of ribosome profiling, disome sequencing and RNA sequenci...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD058232 | Pride
Hiv-1 Ribosome profiling Disome profiling Rna sequencing Lc-ms/ms 
ProteomicsThe E. coli effector EspJ blocks Src kinase activity via amidation and ADP-ribosylation
In vitro ADP-ribosylated Src-K295M was separated by SDS-PAGE and the Coomassie stained protein band excised and digested with either trypsin, thermolysin or elastase. All digests were analysed by nanoLC-MS/MS using Higher-energy C-trap Dissociation (HCD) as well as Electron-Transfer Dissociation (ET...
ORGANISM(S): Homo sapiens (Human) 
2014-12-19 | PXD001461 | Pride
Amidation Src kinase Adp-ribosylation Espj 
ProteomicsProteomic analysis of urine and stone matrix of patients with kidney calculi
Proteins have been extracted from kidney calculi with SDS buffer. Proteins were seperated by 1D-SDS-PAGE and stained with coomassie. Each gel lane was cut into 5 (or 6) pieces. Proteins were digested in gel with trypsin. Tryptic peptides were analysed by nanoLC-MS/MS on an ion trap instrument (6340 ...
ORGANISM(S): Homo sapiens (Human) 
2016-07-11 | PXD000302 | Pride
Inflammation Kidney stone Fibrosis Biomarker 
ProteomicsIdentification of novel canonical and cryptic HCMV-specific T cell epitopes
Infection or reactivation with human cytomegalovirus (HCMV) is still a major cause of morbidity and mortality in patients undergoing solid organ transplantation or allogeneic stem cell transplantation (alloSCT). Recent studies have shown that protection against latent HCMV infection relies on a much...
ORGANISM(S): Homo sapiens (Human) Cytomegalovirus 
2024-06-16 | PXD043022 | Pride
Hcmv Immunopeptidomics Hla peptides 
ProteomicsRNA helicase Ighmbp2 regulates THO complex to ensure cellular mRNA homeostasis
RNA helicases constitute a large protein family implicated in cellular RNA homeostasis and disease development. Here we show that the RNA helicase Ighmbp2, linked to the neuromuscular disorder SMARD1 (DSMA1) associates with polysomes and impacts on translation of cellular mRNAs containing short, GC-...
ORGANISM(S): Homo sapiens (Human) 
2024-02-15 | PXD043336 | Pride
Thoc Mrna-homeostasis Ighmbp2 Smard1 Rna helicase 
ProteomicsSILAC-based quantification reveals modulation of the immunopeptidome in BRAF and MEK inhibitor-treated and resistant tumor cells
The immunopeptidome is constantly monitored by T cells to detect foreign or aberrant HLA peptides. It is highly dynamic and reflects the current cellular state, enabling the immune system to recognize abnormal cellular conditions, such as those present in cancer cells. To precisely determine how cha...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD054097 | Pride
Kinase inhibitor resistance Immunopeptidomics Cancer Mass-spectrometry Cryptic peptides Mapk signaling Melanoma Lc-ms/ms 
ProteomicsInteraction partners of arginyl-tRNA protein transferase in the moss Physcomitrella patens
Protein arginylation is a post-translational modification at the N-terminus of proteins and is crucial for viability and physiology in higher eukaryotes. The lack of arginylation causes severe developmental defects in plants and embryo lethality in Drosophila and in mice. Although several studies in...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2016-04-19 | PXD003228 | Pride
Moss Protein interaction Co-ip Physcomitrella patens Lc-ms/ms 
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