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ProteomicsAnti-FLAG-affinity purification of Umbrea associated factors
Anti-FLAG-affinity purification of Umbrea associated factors from nuclear extracts of Drosophila Schneider cells expressing FLAG-HA-Umbrea. Anti-FLAG -affinity purifications from Schneider cell nuclear extracts not expressing any FLAG-tagged protein served as a control SDS-PAGE separting both sa...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2013-10-03 | PXD000163 | Pride
Fruitfly Umbrea Drosophila melanogaster 
ProteomicsA pair of centromeric proteins mediates reproductive isolation in Drosophila species
Speciation involves the reproductive isolation of natural populations due to the sterility or lethality of their hybrids. However, the molecular basis of hybrid lethality and the evolutionary driving forces that provoke it, remain largely elusive. The hybrid male rescue (Hmr) and the lethal hybrid r...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2013-11-20 | PXD000489 | Pride
Lhr Hmr 
ProteomicsRNase L activating 2′-5′ oligoadenlyates bind ABCF1, -3 and Decr-1
Identification of 2′–5′ OA binding proteins in human (HeLa), mouse (BMDM) and fly (Schneider S2) cells using affinity purification mass spectrometry (AP-MS). Pulsed SILAC-based translational profiling in HeLa and HeLa S3 cells treated with 2′–5′ OA or OH-2′–5′ OA.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Drosophila melanogaster (Fruit fly) 
2023-08-18 | PXD030134 | Pride
Psilac Ap-ms Lc-ms/ms 
ProteomicsComplex regulation of Gephyrin splicing is a determinant of inhibitory postsynaptic diversity
Gephyrin (GPHN) regulates the clustering of postsynaptic components at inhibitory synapses and is involved in pathophysiology of neuropsychiatric disorders. Here, we uncover an extensive diversity of GPHN transcripts that are tightly controlled by splicing during mouse and human brain development. P...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-28 | PXD033751 | Pride
Splice variants Gephyrin Inhibitory synapses 
ProteomicsIdentification of AURORA A interaction partners
The oncogenic kinase AURORA A is essential for mitotic progression, and its catalytic inhibition arrests cells at the G2/M-transition. Unexpectedly, partial degradation of AURORA A by PROTACs (proteolysis targeting chimeras) induces profound S-phase defects, revealing a non-catalytic scaffolding fun...
ORGANISM(S): Homo sapiens (Human) 
2026-06-03 | PXD069843 | Pride
Coip Interactome 
ProteomicsThe 3’-UTR of MYC couples RNA polymerase II function to ribonucleotide levels
Deregulated expression of MYC enhances glutamine utilization and renders cell survival dependent on glutamine, inducing “glutamine addiction”. Surprisingly, colon cancer cells that express high levels of MYC due to WNT pathway mutations, are not glutamine-addicted but undergo a reversible cell cycle...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD005829 | Pride
Myc Mrna-protein interaction Glutamine Nanolc-ms/ms 
ProteomicsDisassembly of Functional Membrane Microdomains Relapses Antibiotic Resistance in Staphylococcus aureus
Bacteria spatially confine cellular processes like protease secretion and signal transduction in membrane platforms termed functional membrane microdomains, which in certain organisational and functional features resemble the lipid rafts of eukaryotic cells. However, a rigorous understanding of thei...
ORGANISM(S): Staphylococcus aureus 
2019-01-08 | PXD006546 | Pride
Drm Dsm Membrane platforms Staphylococcus aureus Lc-ms/ms 
ProteomicsProteomic analysis of urine and stone matrix of patients with kidney calculi
Proteins have been extracted from kidney calculi with SDS buffer. Proteins were seperated by 1D-SDS-PAGE and stained with coomassie. Each gel lane was cut into 5 (or 6) pieces. Proteins were digested in gel with trypsin. Tryptic peptides were analysed by nanoLC-MS/MS on an ion trap instrument (6340 ...
ORGANISM(S): Homo sapiens (Human) 
2016-07-11 | PXD000302 | Pride
Inflammation Kidney stone Fibrosis Biomarker 
ProteomicsRNA helicase Ighmbp2 regulates THO complex to ensure cellular mRNA homeostasis
RNA helicases constitute a large protein family implicated in cellular RNA homeostasis and disease development. Here we show that the RNA helicase Ighmbp2, linked to the neuromuscular disorder SMARD1 (DSMA1) associates with polysomes and impacts on translation of cellular mRNAs containing short, GC-...
ORGANISM(S): Homo sapiens (Human) 
2024-02-15 | PXD043336 | Pride
Thoc Mrna-homeostasis Ighmbp2 Smard1 Rna helicase 
TranscriptomicsEffect of methylphenidate on gene expression profiles of Attention Deficit Hyperactivity Disorder patients and unaffected controls.
Attention deficit hyperactivity disorder (ADHD) is a common psychiatric condition of children with a prevalence of 5-10% worldwide. Up to 30% of adults with a history of childhood ADHD maintain symptoms in later life; these adult ADHD patients are severely impaired in social and professional life d...
ORGANISM(S): Homo sapiens 
2014-09-05 | E-GEOD-52889 | biostudies-arrayexpress
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