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ProteomicsRumen metaproteome - A Structural and Functional Elucidation of the Rumen Microbiome Influenced by Various Diets and Microenvironments
The structure and function of the microbiome inhabiting the rumen are, amongst other factors, mainly shaped by the animal’s feed intake. Describing the influence of different diets on the inherent community arrangement and associated metabolic activities of the most active ruminal fractions (bacteri...
ORGANISM(S): Bacteria Archaea 
2017-08-15 | PXD006070 | Pride
Rumen Metaproteomics Lc-ms/ms 
ProteomicsBacterial and host proteins along and across the porcine GIT
Digesta and mucosa samples from stomach, jejunum, ileum, cecum and colon of the porcine GIT from four animals were analysed by metaproteomics to obtain a deeper insight into the functions of bacterial groups with a concomitant analyses of host proteins.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2019-01-21 | PXD011360 | Pride
Bacteria Pig Metaproetomics 
ProteomicsRumen microbiome and effect of monensin
The time-resolved impact of monensin on the active rumen microbiome in a rumen-simulating technique (Rusitec) was studied with metaproteomic and metabolomic approaches. Upon monensin treatment, decreased catabolism linked to fiber degradation was observed by the reduced abundance of proteins assigne...
ORGANISM(S): Bos taurus (Bovine) 
2022-12-11 | PXD034099 | Pride
Prevotella Microbiome Monensin Rumen 
ProteomicsRumen metaproteomics - Catching the tip of the iceberg - evaluation of sample preparation protocols for metaproteomic studies of the rumen microbiota
Metaproteomic studies of the rumen microbiota are challenged by the need of optimized sample preparation protocols in order to retrieve an enhanced amount of prokaryotic instead of plant and bovine derived cells before protein extraction and subsequent LC-MS/MS analysis. The present study evaluates ...
ORGANISM(S): Eukaryota (eucaryotes) Bacteria Archaea 
2015-04-01 | PXD001526 | Pride
Lc-ms/ms Rumen microbiota Metaproteomics 
ProteomicsMetaproteome of chicken gastrointestinal tract microbiota
The chicken gastrointestinal tract (GIT) harbours a complex microbial community, involved in several physiological processes such as host immunomodulation and feed digestion. Other studies were already performed to define the chicken gut metagenome and its fecal metaproteome. For the first time, the...
ORGANISM(S): Bacteria 
2016-10-06 | PXD003805 | Pride
Microbiota Metaproteome 
Proteomics15N Limitation of detection in Pseudomonas fluorescens
The limit of 15N detection in proteins of Pseudomonas putida was studied by LC-MS/MS. Cells were grown in the presence of 0.1%15N to 10% 15N.The method of protein-based stable isotope probing (protein-SIP) has previously been shown to allow the modeling of carbon fluxes in microbial communities, dem...
ORGANISM(S): Pseudomonas fluorescens 
2013-04-09 | PXD000127 | Pride
Protein-sip 15n Lc-msms 
ProteomicsProteomics of Nitrospirae - Rice paddy Nitrospirae encode and express genes related to sulfate respiration: proposal of the new genus Candidatus Sulfobium
We present the draft genome of Nitrospirae bacterium Nbg-4 as a representative of this clade and couple this to in situ protein expression under sulfate-enriched and sulfate-depleted conditions in rice paddy soil. The proteins were extracted from the soil and analysed via LC-MS/MS measurements.
ORGANISM(S): Bacteria 
2018-01-08 | PXD007817 | Pride
Nitrospirae Soil Bacteria 
Proteomicspig GIT meatproteome - Dietary changes in nutritional studies shape the structural and functional composition of the pigs' fecal microbiome-from days to weeks
Background: The possible impact of changes in diet composition for the intestinal microbiome is mostly studied after some days of adaptation to the diet of interest. The question arises if few days are enough to reflect the microbial response to the diet by changing the community composition and fun...
ORGANISM(S): Bacteria Sus scrofa domesticus (domestic pig) 
2017-10-26 | PXD006224 | Pride
Porcine git Metaproteome Sus scrofa Bacteria Lc/msms 
Proteomicspig microbiota ob/le - Intestinal Microbiota and Microbial Metabolites Are Changed in a Pig Model Fed a High-Fat/Low-Fiber or a Low-Fat/High-Fiber Diet
Feces samples from pigs with two different diets were analysed. Proteins from the microbiota was extracted and analysed by LC-MS/MS. Differences along the time and between the diets were observed.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2016-04-22 | PXD003447 | Pride
Git microbiota Pig 
ProteomicsPrevotella bryantii B14 proteins from in vitro cultivation under various short-chain fatty acids (SCFA)
P. bryantii B14 cells were cultivated separately in acetic (Acet), propionic (Prop), butyric (But), iso-butyric (iBut), valeric (Val), iso-valeric (iVal) and 2-methyl butyric acid (2MB) as well as in a mixture of all mentioned short-chain fatty acids (Mix). All 8 treatments were analyzed regarding t...
ORGANISM(S): Prevotella bryantii B14 Bacteria 
2021-09-09 | PXD016407 | Pride
Prevotella Scfa 
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