Sort   by:  
 Page size 
ProteomicsOn-capillary cell lysis enables top-down proteomic analysis of single HeLa cells by CE-MS/MS
Proteomic analysis of limited samples and single cells requires specialized methods that prioritize high sensitivity and minimize sample loss. Consequently, sample preparation is one of the most important steps in limited sample analysis workflows to prevent sample loss. In this work, we have elimin...
ORGANISM(S): Homo sapiens (Human) 
2022-10-26 | PXD035339 | Pride
Top-down proteomics Single-cell top-down proteomics Tandem mass spectrometry Single-cell proteomics Capillary electrophoresis Ce-ms/ms 
ProteomicsFollicular cells Top-down proteomic
In mammals, the capacity of the female germ cell, the oocyte, to develop into embryo is acquired throughout meiotic maturation. Immature oocyte cannot be fertilized while mature oocyte is apt to accept spermatozoa and to develop an embryo. In a follicle, the oocyte is surrounded by mural granulosa c...
ORGANISM(S): Bos taurus (Bovine) 
2017-04-10 | PXD004892 | Pride
Oocytes Μlc-ms/ms Cumulus cells Top-down proteomic Granulosa cells 
ProteomicsTowards a universal sample preparation method for denaturing top-down proteomics of complex proteomes
A universal and standardized sample preparation method becomes vital for denaturing top-down proteomics (dTDP) to advance the scale and accuracy of proteoform delineation in complex biological systems. It needs to have high protein recovery, minimum bias, good reproducibility, and compatibility with...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-22 | PXD018248 | Pride
Histone Ptms Membrane ultrafiltration Chloroform-methanol precipitation Membrane proteins Sp3 Denaturing top-down proteomics Cze-ms/ms Sds Sample preparation 
ProteomicsBenchmarking multiple fragmentation methods on an Orbitrap Fusion for top-down phospho-proteoform characterization
Top-down analysis of intact proteins by mass spectrometry provides an ideal platform for comprehensive proteoform characterization, in particular, for the identification and localization of post-translational modifications (PTM) co-occurring on a protein. One of the main bottlenecks in top-down prot...
ORGANISM(S): Homo sapiens (Human) 
2015-07-16 | PXD001845 | Pride
Fragmentation Bora Ethcd Top down proteomics Phosphorylation Proteoform Orbitrap fusion 
ProteomicsFunctionally Integrated Top-down Proteomics for Standardized Assessment of Human Induced Pluripotent Stem Cell-derived Engineered Cardiac Tissues
3-dimensional (3D) human induced pluripotent stem cell-derived engineered cardiac tissues (hiPSC-ECTs) have emerged as a promising alternative to 2-dimensional hiPSC-cardiomyocyte monolayer systems because hiPSC-ECTs are a closer representation of endogenous cardiac tissues and more faithfully refle...
ORGANISM(S): Homo sapiens (Human) 
2021-01-07 | PXD022814 | Pride
Top-down proteomics Human pluripotent stem cell Proteoform Engineered cardiac tissue Cardiac function 
UnknownHigh sensitivity top-down proteomics captures single muscle cell heterogeneity in large proteoforms.
Not available
S-EPMC10175728 | biostudies-literature
ProteomicsCombination of digital microfluidics and magnetic bead-based intact proteoform elution for top-down nanoproteomics of single C. elegans
While most nanoproteomics approaches for the analysis of low-input samples are based on bottom-up LC-MS workflows, top-down approaches enabling proteoform characterization are still underrepresented. Using mammalian cell proteomes, we here established a facile one-pot sample preparation protocol bas...
ORGANISM(S): Caenorhabditis elegans 
2023-06-19 | PXD039883 | Pride
Top-down proteomics  nanoproteomics  caenorhabditis elegans  digital microfluidics 
ProteomicsCysteine-directed isobaric labeling combined with GeLC-FAIMS MS for quantitative top-down proteomics
The quantification of proteoforms, i.e., all molecular forms in which proteins can be present, by top-down proteomics provides essential insights into biological processes at the molecular level. Isobaric labeling-based quantification strategies are suitable for multi-dimensional separation strategi...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2025-05-07 | PXD055510 | Pride
Top-down proteomics  tmt labeling  quantitative proteomics  faims  gelc  peppi 
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard s...
ORGANISM(S): Homo Sapiens (human) 
2025-01-23 | PXD056413 | JPOST Repository
ProteomicsThe Isotopic Depletion Increases the Spatial Resolution of FPOP Top-Down Analysis.
Protein radical labeling offers an alternative analytical method for probing protein structure or protein interaction with other bio-molecules. Since the Fast Photochemical Oxidation of Proteins has already shown its essential role in studying biomolecular as-semblies and it was successfully adopted...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD044447 | Pride
Protein isotope depletion Ire Fast photochemical oxidation of proteins Mass spectrometry Insulin response element Top-down Foxo4 Electron-capture dissociation Multicasi. 
Sort   by:  
 Page size