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ProteomicsStructural and mechanistic insights into the CAND1-mediated SCF substrate receptor exchange
Modular SCF (SKP1-CUL1-Fbox) ubiquitin E3 ligases orchestrate multiple cellular pathways in eukaryotes. Their variable SKP1-Fbox substrate receptor (SR) modules enable regulated substrate recruitment and subsequent proteasomal degradation. CAND proteins are essential for the efficient and timely exc...
ORGANISM(S): Homo sapiens (Human) 
2023-09-18 | PXD042178 | Pride
Human Cand1 Cryo-em Hdx-ms 
ProteomicsShulin packages axonemal outer dynein arms for ciliary targeting
The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During cilio-genesis, these ∼1.8 MDa complexes are assembled in the cytoplasm and targeted to cilia via an unknown mechanism. Here we use the ciliate Tetrahymena to identify two novel factors (Q22YU3 an...
ORGANISM(S): Tetrahymena thermophila CU428 
2021-09-09 | PXD022396 | Pride
Cilia Tetrahymena thermophila Dynein Cryo-em Lc-ms/ms Flagella 
ProteomicsMALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity
The interaction partners of C. elegans soluble IL-17 signaling components were profiled using IP/MS. In vivo interactions were profiled for ACTL-1-FLAG, PIK-1-Myc, MALT-1::GFP, NFKI-1::GFP and their respective controls. Quantification of NFKI-1::GFP interactions was performed by labelling immunoprec...
ORGANISM(S): Caenorhabditis elegans 
2020-05-27 | PXD018000 | Pride
C. elegans Proteomics Neuroimmune communication Behaviour Signaling 
ProteomicsBacillus subtilis YtpP and thioredoxin A are new players in the CoA-mediated defense mechanism against cellular stress
Coenzyme A is an important cellular metabolite critical for metabolic processes and the regulation of gene expression. The recent discovery of its antioxidant role has highlighted its protective function that leads to the formation of a mixed-disulfide bond with protein cysteines, termed protein CoA...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. L170 
2023-05-10 | PXD041347 | Pride
Bacillus megaterium Coenzyme a Bacillus subtilis Antioxidant Oxidative stress Mixed-disulfide Protein coalation Protein decoalation 
ProteomicsNucleocytoplasmic mRNA redistribution accompanies RNA binding protein mislocalisation in ALS motor neurons and is restored by VCP inhibition
Although the pathological hallmark of amyotrophic lateral sclerosis (ALS) is the nucleocytoplasmic mislocalisation of RNA binding proteins (RBPs), such as TDP-43 and FUS, the nucleocytoplasmic distribution of mRNA remains uncharacterised. Here, we used subcellular fractionation with RNA sequencing a...
ORGANISM(S): Homo sapiens (Human) 
2023-10-13 | PXD037107 | Pride
Human Ipsc Als Motor neuron Lc-ms/ms 
ProteomicsStructural bases for integrase-RNA binding and retention of viral RNA within HIV-1 capsid cores
HIV-1 integrase (IN) promotes encapsulation of viral genomic RNA into mature viral cores, and this function is a target for ongoing antiretroviral drug development efforts. We determined a cryo-EM structure of a primate lentiviral IN complexed with RNA, which revealed a linear filament made of IN oc...
ORGANISM(S): Simian immunodeficiency virus 
2025-12-04 | PXD070910 | Pride
Hiv-1 Hdx/ms Integrase Human Cryoem 
ProteomicsMulti-signal regulation of the GSK-3β homolog Rim11 governs meiosis entry in yeast
Starvation in diploid budding yeast cells triggers a cell-fate program culminating in meiosis and spore formation. Transcription activation of early meiotic genes (EMGs) hinges on the transcription activator Ime1, its DNA-binding partner Ume6, and GSK-3 kinase Rim11. Phosphorylation of Ume6 by Rim11...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-03-06 | PXD049212 | Pride
Yeast Meiosis Phosphorylation Kinase 
ProteomicsThe E3 ubiquitin ligase HECTD1 contributes to cell proliferation through an effect on mitosis
Mechanistic studies of how protein ubiquitylation regulates the cell cycle, in particular during mitosis, have provided unique insights which have contributed to the emergence of the ‘Ubiquitin code’. In contrast to RING E3 ubiquitin ligases such as the APC/c ligase complex, the contribution of othe...
ORGANISM(S): Homo sapiens (Human) 
2022-08-03 | PXD035503 | Pride
Ubiquitin E3 ubiquitin ligase Cell proliferation Trabid Hectd1 Cell cycle 
ProteomicsPost-gastrulation amnioids (PGAs) as a stem cell-derived model of week 2-4 of human extra-embryonic development
The amnion, an essential extra-embryonic tissue in mammalian embryos, is thought to provide crucial signaling, structural and nutritional support during pregnancy. Despite its pivotal importance, studying primate amnion formation and function has been hampered by the lack of accurate in-vitro models...
ORGANISM(S): Homo sapiens (Human) 
2026-01-19 | PXD062917 | Pride
Development Stem cell Aminoids Lc-ms/ms 
ProteomicsActivation loop phosphorylation and cGMP saturation of PKG regulate egress of malaria parasites
The cGMP-dependent protein kinase (PKG) is the sole cGMP sensor in malaria parasites, acting as an essential signalling hub to govern key developmental processes throughout the parasite life cycle. Despite the importance of PKG in the clinically relevant asexual blood stages, many aspects of malaria...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2024-06-24 | PXD049017 | Pride
Phosphorylation Cgmp-dependent protein kinase Malaria Genetics Plasmodium falciparum 
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