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ProteomicsQualitative and quantitative top-down proteomics of human colorectal cancer cell lines identified 23000 proteoforms and revealed drastic proteoform-level differences between metastatic and non-metastatic cancer cells
Understanding cancer metastasis at the proteoform level is crucial for discovering new protein biomarkers for cancer diagnosis and drug development. Proteins are the primary effectors of function in biology and proteoforms from the same gene can have drastically different biological functions. Here,...
ORGANISM(S): Homo sapiens (Human) 
2023-01-05 | PXD029703 | Pride
Top-down proteomics Proteoform Cancer metastasis Colorectal cancer 
ProteomicsA pilot study for deciphering post-translational modifications and proteoforms of tau protein by capillary electrophoresis-mass spectrometry
Abnormal accumulation of tau proteins is one pathological hallmark of Alzheimer’s disease (AD). Many tau protein post-translational modifications (PTMs) are associated with the development of AD, such as phosphorylation, acetylation, and methylation. Therefore, a complete picture of PTM landscape of...
ORGANISM(S): Escherichia coli 
2024-10-15 | PXD053367 | Pride
Alzheimer’s disease Capillary electrophoresis-mass spectrometry Post-translational modification Tau protein Phosphorylation Proteoform 
ProteomicsQuantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in Xenopus laevis
The capacity to regenerate the spinal cord after an injury is a coveted trait that only a limited group of non-mammalian organisms can achieve. In Xenopus laevis, this capacity is only present during larval or tadpole stages, but is absent during postmetamorphic frog stages. This provides an excelle...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2018-01-25 | PXD006993 | Pride
Spinal cord injury Regenerative and a non-regenerative stage Quantitative proteomics Xenopus laevis 
ProteomicsQuantitative top-down proteomics reveals significant differences in histone proteoforms between metastatic and nonmetastatic colorectal cancer cells
As one of the leading causes of cancer deaths worldwide, colorectal cancer (CRC) development is closely associated with the accumulation of both genetic and epigenetic alterations. Many efforts have been made to investigate the role of epigenetic modifications in CRC metastasis. In this work, we pre...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD060160 | Pride
Top-down proteomics Post-translational modifications Capillary electrophoresis-mass spectrometry Colorectal cancer metastasis Histone proteoforms 
ProteomicsA simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP) in recent years. CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-15 | PXD051862 | Pride
Top-down proteomics  proteoform  capillary zone electrophoresis-mass spectrometry  covalent cationic coating  reproducibility  large proteoforms 
ProteomicsLarge-scale Top-down Proteomics of the Arabidopsis thaliana Leaf and Chloroplast Proteomes
We present a large-scale top-down proteomics study of plant leaf and chloroplast proteins, achieving the identification of over 4700 unique proteoforms. Using capillary zone electrophoresis coupled with tandem mass spectrometry analysis of offline size-exclusion chromatography fractions, we identify...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-01-05 | PXD034368 | Pride
Arabidopsis thaliana Post-translational modification Proteoform Transit peptide Singlet oxygen Chloroplast 
ProteomicsAn improved capillary isoelectric focusing-mass spectrometry method for characterization of monoclonal antibody charge variants
Here, we improved the stability and enhanced separation resolution of automated cIEF-MS by bettering the quality of capillary neutral coating, reducing catholyte pH to 10 for cIEF-MS for the first time, and systematically optimizing the cIEF separation conditions for characterization of monoclonal a...
ORGANISM(S): Homo sapiens (Human) 
2026-08-19 | PXD082799 | Pride
Monoclonal antibody charge variants Cief-ms 
ProteomicsNanoparticle-aided nanoreactor for large-scale proteomics of few mammalian cells
Large-scale bottom-up proteomics of few even single cells is crucial for a better understanding of the roles played by cell-to-cell heterogeneity in disease and development. Novel proteomic methodologies with extremely high sensitivity are required for few even single-cell proteomics. Sample process...
ORGANISM(S): Homo sapiens (Human) 
2022-07-11 | PXD027135 | Pride
Nanoreactor Single-cell proteomics Mass-limited sample Nanoproteomics Bottom-up proteomics Nanoparticle Few-cell proteomics 
ProteomicsTowards a universal sample preparation method for denaturing top-down proteomics of complex proteomes
A universal and standardized sample preparation method becomes vital for denaturing top-down proteomics (dTDP) to advance the scale and accuracy of proteoform delineation in complex biological systems. It needs to have high protein recovery, minimum bias, good reproducibility, and compatibility with...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-22 | PXD018248 | Pride
Histone Ptms Membrane ultrafiltration Chloroform-methanol precipitation Membrane proteins Sp3 Denaturing top-down proteomics Cze-ms/ms Sds Sample preparation 
ProteomicsSolid phase microextraction-aided capillary zone electrophoresis-mass spectrometry: ready for bottom-up proteomics of single human cells
Capillary zone electrophoresis-mass spectrometry (CZE-MS) has been recognized as a valuable technique for proteomics of mass-limited biological samples (i.e., single cells) due to its high efficiency and high sensitivity for peptide separation and detection. However, its broad adoption for single ce...
ORGANISM(S): Homo sapiens (Human) 
2024-05-01 | PXD047627 | Pride
Hela Single cell proteomics Mass limited Solid phase microextraction Mass spectrometry Bottom-up proteomics 
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