Sort   by:  
 Page size 
UnknownRevisiting the phosphotyrosine binding pocket of Fyn SH2 domain led to the identification of novel SH2 superbinders.
Not available
S-EPMC7888540 | biostudies-literature
ProteomicsEngineering human SH2 domains for targeted phospho-proteomics
A comprehensive analysis of the phosphoproteome is essential for understanding molecular mechanisms of human diseases. However, current tools to enrich phosphotyrosine are limited in their applicability and scope. Here, we engineered new superbinder SH2 domains that enrich diverse sets of phosphotyr...
ORGANISM(S): Homo sapiens (Human) 
2023-01-13 | PXD030038 | Pride
Phosphoproteomics Synthetic biology K562 Src homology 2 domains Sh2 superbinders Mass spectrometry Human Structural biology Protein engineering Phosphotyrosine 
ProteomicsPatient-derived KEAP1 superbinder mutants stabilize KEAP1 structure and sequester NRF2 in p62-dependent clusters
Cancer-derived loss-of-function mutations in the KEAP1 tumor suppressor gene stabilize the NRF2 transcription factor, resulting in a pro-survival gene expression program that alters cellular metabolism and neutralizes oxidative stress. In a previous study of KEAP1 mutations observed in lung cancer, ...
ORGANISM(S): Homo sapiens (Human) 
2018-11-07 | PXD008411 | Pride
Nrf2 Keap1 Superbinders P62 Clusters Mutations Lung cancer 
UnknownEngineered SH2 Domains for Targeted Phosphoproteomics.
Not available
S-EPMC9251651 | biostudies-literature
UnknownEngineering of Src Homology 2 Domain Leading to Sulfotyrosine Recognition With a High Affinity by Integrating a Distinctive Selection Theme and Next-Generation Sequencing.
Not available
S-EPMC9204161 | biostudies-literature
ProteomicsAutomated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics
Phosphotyrosine (pY) enrichment is critical for expanding fundamental and clinical understanding of cellular signaling by mass spectrometry-based proteomics. However, current pY enrichment methods exhibit a high cost per sample and limited reproducibility due to expensive affinity reagents and manua...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD041271 | Pride
Hela Lc-msms Kingfisher Orbitrap eclipse Orbitrap exploris 480 Phosphotyrosine enrichment 
ProteomicsA sensitive and cost-effective approach for tyrosine phosphoproteome analysis based on SH2 superbinder
Albeit much less abundant than Ser/Thr phosphorylation (pSer/pThr), Tyr phosphorylation (pTyr) is considered a hallmark in cellular signal transduction. However, its analysis remains a challenge. The conventional immunopurification (IP) approach using antibodies pan-specific to pTyr sites is known t...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-08-29 | PXD005838 | Pride
Lc ms/ms Sh2 superbinder Ptyr phosphoproteome 
ProteomicsProteome and phosphoproteome signatures of recurrence for HPV+ head and neck squamous cell carcinoma
Head and neck squamous cell carcinoma (HNSCC) is the 6th most common cancer worldwide and the human papillomavirus (HPVP+P)-driven subtype is the fastest rising in North America. Although most cases of HPV+ HNSCC respond to radiation and chemotherapy in combination with surgery, up to 20% of patient...
ORGANISM(S): Homo sapiens (Human) 
2022-06-01 | PXD030343 | Pride
Proteomics Mass spectrometry Recurrence Human papillomavirus Fibrosis Phosphoproteomics Tumor microenvironment Immune landscape Head and neck squamous cell carcinoma 
Sort   by:  
 Page size