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ProteomicsBenchmarking LFQ, SILAC and MS2/MS3-based TMT quantification strategies for large-scale phosphoproteomics
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-03-13 | PXD007145 | Pride
Phosphoproteomics Ms3 Ms2 Tmt Silac Lfq Technical Benchmark Quantification 
ProteomicsTMT-based Multiplexed (Chemo)proteomics on the Orbitrap Astral Mass Spectrometer
This dataset contains an evaluation of TMT-based multiplexed proteomics on the Orbitrap Astral mass spectrometer.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-17 | MSV000096674 | MassIVE
ProteomicsComparison of LFQ and TMT-based quantitative proteomics towards toxicology research
Here, we aimed to compare two of the most commonly used global proteomics strategies, label-free quantification (LFQ) and tandem mass tag-based quantification (TMT) to identify the most suitable tool for toxicology research questions on the systemic level. Using a biological case study of HepG2 expo...
ORGANISM(S): Homo sapiens (Human) 
2020-12-23 | PXD020248 | Pride
Toxicoproteomics Benzo[a]pyrene Tandem mass tag (tmt) Label free-quantification (lfq) Hepg2 
ProteomicsLiver tissue proteomics with TMT-based quantification
Liver tissue proteomics with TMT-based quantification
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-08-03 | MSV000098710 | MassIVE
ProteomicsSerum proteome profiling in canine chronic valve disease using TMT-based quantitative proteomics
Cardiovascular-related disorders (CVD) account for the highest mortality rates in the world, and affect the quality and the quantity of life of patients, creating an economic burden of prolonged therapeutic intervention. The aim of this study was to evaluate the serum protein profile of healthy and ...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2020-05-26 | PXD017129 | Pride
Dog Tandem mass tag Chronic valve disease Serum proteomics 
ProteomicsmTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-base...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014545 | Pride
Silac Tmt Isobaric Sh-sy5y Svgp12 Metabolic 
ProteomicsTMT and Label-Free Quantification of Yeast Mitochondrial and Cellular Proteome During Different Stages of Growth
Tandem mass tag (TMT)-based relative quantification was used in combination with the intensity-based absolute quantity estimation (IBAQ) to study of both yeast cells and isolated yeast mitochondria during fermentative growth, diauxic shift, respiratory growth.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-06 | PXD012802 | Pride
Lc-ms Intensity-based absolute quantification Saccharomyces cerevisiae Label-free quantification Tandem mass tag Diauxic shift 
ProteomicsRatio accuracy evaluation and correction using tandem mass tags
The multiplexing capabilities of isobaric mass tag based protein quantification, such as Tandem Mass Tags (TMT) or Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) have dramatically increased the scope of Mass Spectrometry (MS) based proteomics studies. Not only does the technology allow ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-27 | MSV000082855 | MassIVE
UnknownBenchmarking common quantification strategies for large-scale phosphoproteomics.
Not available
S-EPMC5849679 | biostudies-literature
ProteomicsTMT-based study of yeast metabolism and translation under nitrogen limitation
Tandem mass tag (TMT)-based relative quantification was used to study yeast metabolism under the stepwise reduction the nitrogen content in the growth medium.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-13 | PXD014962 | Pride
Metabolism Yeast Tmt Tandem mass tag Orbitrap fusion 
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