We generated the raw sample sets of TMT16-labeled for the quantification of single-cell from two cultured murine cell lines, GC-1 spg (GC-1) and GC-2spd (GC-2), and the raw bulk sets of TMT16-labeled cells.
We combined pulsed stable isotope labeling of amino acids (pSILAC) with enrichment of phosphorylated peptides in a method we term PhosphoProtein-Peptide Turnover Profiling (PhosphoPPToP). We applied the method to HeLa cells to find peptides whose clearance rates differ from the protein median. Using...
Systematically optimized the protocol for the newly developed 16-plex TMT Reported a novel 27-plex method to enhance the multiplexity for large-scale proteomic profiling, by combining two sets of distinct isobaric tags (11-plex and 16-plex TMT) based on their disparate mass shifts on modified peptid...
With HiRIEF LC-MS/MS, we analysed 11 GBM stem cell lines from the HGCC cohort (Uppsala, Sweden). The samples were run in one TMT16 set, with three samples analysed in duplicates, and the last two channels left empty.
Autophagy is a constitutive process of lysosomal degradation required for maintaining the homeostasis of large molecular complexes and organelles in neurons. Here we reported a systemic investigation of neuronal autophagy targets through integrated proteomics and functional analysis. Proteomic profi...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse)
Here, we present an in-depth proteomic profiling of pre-diagnostic plasma samples from 114 individuals with suspected lung cancer, including 57 patients diagnosed with stage III-IV lung adenocarcinoma (LUAD) and 57 non-cancer controls. Plasma samples were depleted of high-abundant proteins, and prot...
Neuroblastoma accounts for 15% of cancer-related deaths in children, highlighting an unmet need for novel therapies. Selinexor is a small molecule inhibitor of XPO1 that shuffles cargo proteins with a nuclear export sequence, many of which are essential for cancer growth and cell maintenance, from t...
