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ProteomicsQuantitative TMT proteomics of myristoylated proteins in Trypanosoma cruzi and human cardiomyocytes during N-myristoyltransferase inhibition
This project investigates the impact of N-myristoyltransferase (NMT) inhibition on the myristoylated proteome of Trypanosoma cruzi and human cardiomyocytes in the context of Chagas disease. Human AC16 cardiomyocytes were infected with T. cruzi (CL Brener strain) and treated with the reference NMT in...
ORGANISM(S): Trypanosoma cruzi Homo sapiens (Human) 
2026-01-26 | PXD071927 | Pride
N-myristoyltransferase N-myristoylation Tmt sixplex Orbitrap exploris 240 Chagas disease Ac16 cardiomyocytes Trypanosoma cruzi Click chemistry Quantitative proteomics Lc–ms/ms 
ProteomicsGlobal proteomics of HEK293T cells treated with Val-boroPro for 48h
Global proteomics of HEK293T cells treated with Val-boroPro for 48h
ORGANISM(S): Homo sapiens (Human) 
2020-10-14 | PXD015978 | Pride
Val-boropro Tmtsixplex 
ProteomicsTherapeutic inhibition of acid sensing ion channel 1a recovers heart function after ischemia-reperfusion injury
Background: Ischemia-reperfusion injury (IRI) is one of the major risk factors implicated in morbidity and mortality associated with cardiovascular disease. During cardiac ischemia, the build-up of acidic metabolites results in decreased intracellular and extracellular pH that can reach as low as 6....
ORGANISM(S): Mus musculus (Mouse) Rattus norvegicus (Rat) 
2022-11-01 | PXD024581 | Pride
Heart Venom Mass spectrometry Therapeutic Human induced pluripotent stem cells Ischemia-reperfusion injury 
ProteomicsMouse,GC-1,TMT（Tandem Mass Tag）tag quantification
Tandem Mass Tag (TMT) technology is an in vitro peptide labeling technique developed by Thermo Fisher Scientific, a peptide labeling technology developed in vitro. The technology uses six (TMTsixplex™ Isobaric Label Reagent Set) or 10 (TMT10plex™ Isobaric Label Reagent Set) isotope labels by labelin...
ORGANISM(S): Mus musculus (Mouse) 
2022-01-19 | PXD030967 | Pride
Gc-1 Mouse 
ProteomicsOxidized thioredoxin-1 restrains the NLRP1 inflammasome
To find cellular protein binding partners of the NLRP1 inflammasome, FLAG-tagged full length NLRP1 (or a GFP-FLAG protein control) was ectopically expressed in HEK 293T cells and affinity purified from cell lysates (Note: the NLRP1-FLAG-containing lysate samples were affinity purified in the presenc...
ORGANISM(S): Homo sapiens (Human) 
2022-11-04 | PXD037391 | Pride
Innate immunity Programmed cell death Inflammasome Chemical biology Pattern recognition receptors 
ProteomicsTMT-based quantitative MS analysis of proteome labeled by NO-responsive reaction with NOAR-2
RAW264.7 cells were treated by LPS and IFN-gamma for 20 h. The LPS/IFN treated or nontreated cells were labeled by NOAR-2 for 1 h. Cells were lysed by NP-40 buffer. Labeled proteins were purified by immunopecipitation using anti-fluorescein, then digested by trypsin. Digested peptides were labeled ...
ORGANISM(S): Mus Musculus (mouse) 
2020-01-12 | PXD012310 | JPOST Repository
ProteomicsDevelopment of nucleus-targeted synthetic histone photoaffinity probes
Dissection of the physiological interactomes of histone post-translational modifications (hPTMs) is crucial for understanding fundamental epigenetic regulatory pathways. Current peptide- or protein-based histone photoaffinity probes have greatly expanded the ability to probe the epigenetic interacto...
ORGANISM(S): Mus musculus (Mouse) 
2024-11-21 | PXD045620 | Pride
Nucleus-targeted histone photoaffinity probe Lactylation The epigenetic interactome Hard-to-transfect cells Native cells 
ProteomicsDPP9 activity and not protein binding inhibits the CARD8 inflammasome
Inflammasomes are multiprotein complexes formed in response to pathogens. NLRP1 and CARD8 are related proteins that form inflammasomes, but the pathogen-associated signal(s) and the molecular mechanisms controlling their activation have not been established. Inhibitors of the serine dipeptidyl pept...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD015047 | Pride
Val-boropro Card8 Dpp9 
ProteomicsThrombo-Inflammation in Preeclampsia and Pregnancy Induced Hypertension: Proteomics And Metabolomics Profiling of Platelets and Plasma Mediators
Background: Platelets may be pivotal mediators of the thrombo-haemorrhagic complications of preeclampsia (PE), linking inflammation and thrombosis with endothelial and vascular dysfunction. While gestational hypertension (GH) falls within the spectrum of hypertensive complications of pregnancy and i...
ORGANISM(S): Homo sapiens (Human) 
2022-03-16 | PXD031278 | Pride
Metabolomics String bg Preeclampsia Pregnancy Gestational hypertension Proteomics Pathway analysis Lrrc27/42 
ProteomicsUrinary proteome in diabetic nephropathy
Urinary proteome was analyzed and quantified by Tandem Mass Tag (TMT) labeling followed by bioinformatics analysis to study DN pathophysiology and to identify biomarkers of clinical outcome. We included type 2 diabetic normotensive non-obese males with and without incipient DN (microalbuminuria). Sa...
ORGANISM(S): Homo sapiens (Human) 
2018-05-22 | PXD009303 | Pride
Human Urinary proteome Quantitative proteomics Diabetes 
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