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ProteomicsInteractome analysis of GST-UFD1-NBM
GST pull-down assay using crude extracts from HCT116 cells to analyze the selectivity of the UFD1-NBM peptide in a cellular context.
ORGANISM(S): Homo sapiens (Human) 
2025-06-03 | PXD063521 | Pride
Vcp Ufd1 Npl4 P97 
ProteomicsFAF2 enhances unfoldase activity of mammalian p97-UFD1-NPL4 complex enabling rational design of p97
To address how adaptor proteins impact human p97 activity, we here employ in vitro reconstitution and a focused biochemical screen to identify adaptors that enhance unfoldase activity of human p97 in the presence of UFD1-NPL4. Of the adaptors tested, Fas-associated factor 2 (FAF2/UBXD8), a UBX domai...
ORGANISM(S): Homo sapiens (Human) 
2026-07-28 | PXD073479 | Pride
Faf2 Hdx-ms P97 
ProteomicsFAF1 and FAF2 enhance human p97-UFD1-NPL4 complex unfoldase activity enabling rational design of p97 activators - Crosslinking mass spec data
VCP/p97 is an AAA+ ATPase that, together with its cofactors UFD1-NPL4 (p97-UN), binds and unfolds ubiquitylated substrates to maintain cellular homeostasis. The human p97-UN complex associates with additional cofactors, but how these cofactors modulate p97-UN activity is not fully understood. We scr...
ORGANISM(S): Homo sapiens (Human) 
2026-07-28 | PXD078865 | Pride
Atpase Xl-ms Faf2 Ufd1 Vcp Npl4 P97 
ProteomicsTargeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast
In eukaryotes, the conjugation of proteins to the small ubiquitin-like modifier SUMO regulates numerous cellular functions. A proportion of SUMO conjugates are targeted for degradation by SUMO-targeted ubiquitin ligases (STUbLs) and it has been proposed that the ubiquitin-selective chaperone Cdc48/p...
ORGANISM(S): Schizosaccharomyces pombe 
2015-10-30 | PXD002972 | Pride
Lc-msms S. pombe Stubl Cdc48-ufd1-npl4 Sumo modification sites 
UnknownStructural insights into the p97-Ufd1-Npl4 complex.
Not available
S-EPMC1761865 | biostudies-literature
ProteomicsInduced degradation of Ufd1 reveals regulation of cohesin by the VCP/p97Ufd1-Npl4 complex
The AAA ATPase VCP/p97 has emerged as a critical regulator of ubiquitin and chromatin-associated processes but progress in understanding has been hampered by the complexity of p97 functions and the various p97 cofactors involved. Here, we combined ubiquitin profiling with acutely induced degradation...
ORGANISM(S): Homo sapiens (Human) 
2026-08-31 | PXD082712 | Pride
Dna damage Replication Ubiquitin Vcp Cohesin 
UnknownMultiple UBX proteins reduce the ubiquitin threshold of the mammalian p97-UFD1-NPL4 unfoldase.
Not available
S-EPMC9377798 | biostudies-literature
UnknownDistinct conformations of the protein complex p97-Ufd1-Npl4 revealed by electron cryomicroscopy.
Not available
S-EPMC3268311 | biostudies-literature
UnknownStructural insights into ubiquitin recognition and Ufd1 interaction of Npl4.
Not available
S-EPMC6910952 | biostudies-literature
UnknownSUMO enhances unfolding of SUMO-polyubiquitin-modified substrates by the Ufd1/Npl4/Cdc48 complex.
Not available
S-EPMC9910466 | biostudies-literature
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