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Knowing that biochemically RLPH2 is a tyrosine phosphatase, we selectively In an attempt to discover RLPH2 substrates we decided to use the power of phospho-proteomics. Immunoprecipitated tyrosine phosphorylated peptides from enriched phospho-peptide pools derived from these plant extracts. Mass spe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-01-26 | PXD038689 | Pride
Clubroot disease, caused by Plasmodiophora brassicae, poses a significant global threat to Brassica crop production. Understanding the disease resistance mechanism is vital to assist in the continuous development of clubroot-resistant genetic material. In this study, we used clubroot-resistant (CR) ...
ORGANISM(S): Brassica napus (Rape) 
2025-10-06 | PXD060262 | Pride
Protein kinases are key components of multiple cell signaling pathways. Several protein kinases of the receptor-like cytoplasmic kinase (RLCK) family have demonstrated roles in immune and developmental signaling across various plant species, making them a family of interest in the study of phosphory...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-07 | PXD052679 | Pride
Phosphorus (P) is an essential plant macronutrient required for fundamental biochemical, metabolic, and physiological processes. In its inorganic and chemically available form of orthophosphate (H2PO4-; Pi), plant root epidermal cells are able to directly assimilate this mineral from the soil via pl...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-09 | PXD062427 | Pride
Increasing global food production demands have resulted in increased fertilizer usage, causing detrimental environmental impacts. Biostimulants, such as humic substances, are currently being applied as a strategy to increase plant nutrient-use efficiency and minimize environmental impacts within cro...
ORGANISM(S): Triticum aestivum (Wheat) 
2025-07-28 | PXD058950 | Pride
The calcium-dependent protein kinase CPK28 is a regulator of immune homeostasis in multiple plant species. Here, we used a proteomics approach to uncover CPK28-associated proteins. We found that CPK28 associates with subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39, and trans-phosphorylates RAF2...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-09-30 | PXD052803 | Pride
In the evolving landscape of horticultural science, light emitting diodes (LEDs) present an innovative method for manipulating plant growth and development. Indeed, light serves as a fundamental energy source and an environmental cue for plant life, providing us an ability to control essential plant...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-09-14 | PXD055752 | Pride
A major limitation in quantitative time-course proteomics is the tradeoff between depth-of-analysis and speed-of-analysis. In high complexity, high dynamic samples such as plant extracts, this is tradeoff is especially apparent. To address this, we evaluate multiple composition voltage (CV) High Fie...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-26 | PXD038989 | Pride
Plants, as sessile organisms, require robust responses in protein synthesis to adapt to variable environmental conditions. Measurement of newly synthesized proteins has been successfully facilitated with Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT) across a multitude of organisms. Here, ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-06-08 | PXD064210 | Pride
Outer Membrane Vesicles (OMVs) are spherical bodies derived from the outer membrane of Gram-negative bacteria. Fully encapsulated by membrane, these extracellular structures transport cargo, often proteins, from within the bacterium into the external environment. Although there are multiple studies ...
ORGANISM(S): Methylomicrobium album BG8 
2026-05-25 | PXD050176 | Pride
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