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Knowing that biochemically RLPH2 is a tyrosine phosphatase, we selectively In an attempt to discover RLPH2 substrates we decided to use the power of phospho-proteomics. Immunoprecipitated tyrosine phosphorylated peptides from enriched phospho-peptide pools derived from these plant extracts. Mass spe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-01-26 | PXD038689 | Pride
The timing of flowering is a critical agronomic trait governed by a number of different internal and external events, such as temperature, light and photoperiod. Despite a firm understanding of flowering time from a genetic standpoint, there exists a limited understanding of how post-translational m...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-09 | PXD038688 | Pride
Protein kinases are key components of multiple cell signaling pathways. Several protein kinases of the receptor-like cytoplasmic kinase (RLCK) family have demonstrated roles in immune and developmental signaling across various plant species, making them a family of interest in the study of phosphory...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-07 | PXD052679 | Pride
Clubroot disease, caused by Plasmodiophora brassicae, poses a significant global threat to Brassica crop production. Understanding the disease resistance mechanism is vital to assist in the continuous development of clubroot-resistant genetic material. In this study, we used clubroot-resistant (CR) ...
ORGANISM(S): Brassica napus (Rape) 
2025-10-06 | PXD060262 | Pride
Appropriate soil nutrient management is critical for modern Canola (Brassica napus L.) varieties and hybrids to meet their yield potentials. Canola fields are typically supplemented by the application of nitrogen, phosphorus, sulphur, and to a lesser extent, potassium fertilizers to maximize yield, ...
ORGANISM(S): Brassica napus (Rape) 
2025-05-06 | PXD032223 | Pride
A major limitation in quantitative time-course proteomics is the tradeoff between depth-of-analysis and speed-of-analysis. In high complexity, high dynamic samples such as plant extracts, this is tradeoff is especially apparent. To address this, we evaluate multiple composition voltage (CV) High Fie...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-26 | PXD039004 | Pride
Clubroot of Brassicaceae, an economically important soil borne disease, is caused by Plasmodiophora brassicae Woronin, an obligate, biotrophic protist. This disease poses a serious threat to canola and related crops in Canada and around the globe causing significant loss to seed yield. The pathogen ...
ORGANISM(S): Brassica napus (Rape) 
2022-03-22 | PXD031070 | Pride
Outer Membrane Vesicles (OMVs) are spherical bodies derived from the outer membrane of Gram-negative bacteria. Fully encapsulated by membrane, these extracellular structures transport cargo, often proteins, from within the bacterium into the external environment. Although there are multiple studies ...
ORGANISM(S): Methylomicrobium album BG8 
2026-05-25 | PXD050176 | Pride
Drought is a destructive abiotic stress, with plants possessing diverse strategies to survive periods of limited water resources. Previous results have described connections between strigolactone (SL) and drought, however, here we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf1...
ORGANISM(S): Hordeum vulgare (Barley) 
2023-08-31 | PXD040828 | Pride
Phosphorus (P) is an essential plant macronutrient required for fundamental biochemical, metabolic, and physiological processes. In its inorganic and chemically available form of orthophosphate (H2PO4-; Pi), plant root epidermal cells are able to directly assimilate this mineral from the soil via pl...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-09 | PXD062427 | Pride
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