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TranscriptomicsOncogenic Myc levels activate and repress specific genes to form characteristic expression profiles in human tumors
Myc was depleted in HeLa cells (ATCC CRL-1934) with an shRNA and the resulting expression profile changes mapped via microarrays. This study is the equivalent of E-MTAB-1524 . E-MTAB-1524 has been replaced by E-MTAB-1886.
ORGANISM(S): Homo sapiens 
2014-07-11 | E-MTAB-1886 | biostudies-arrayexpress
ProteomicsInteractome of chromatin-associated RNA Polymerase II from U2OS cells
To determine the interactome of RNA Polymerase II (RNAPII), HA-tagged RPB3 was stably expressed in U2OS cells. The cells were harvested followed by the native stepwise isolation of chromatin-associated RNAPII complexes. HA-RPB3 was immunoprecipitated and interacting proteins analyzed by quantitative...
ORGANISM(S): Homo sapiens (Human) 
2024-12-20 | PXD054701 | Pride
Interactome U2os Rnapii 
ProteomicsRNA Polymerase II interactome in presence or absence of SCAF1 in U2OS-SCAF1-AID cells
Transcripts produced by RNA polymerase II (RNAPII) are fundamental for cellular responses to environmental changes. It is therefore no surprise that there exist multiple avenues for the regulation of this process. To explore the regulation mediated by RNAPII-interacting proteins, we used an siRNA-ba...
ORGANISM(S): Homo sapiens (Human) 
2024-12-20 | PXD054675 | Pride
Scaf1-depletion Interactome Rnapii 
ProteomicsProteomics analysis with the Aurora-A PROTAC, JB170
To determine specificity of Aurora-A PROTAC (JB170), MV4-11 cells were labeled with medium containing different stable isotopes (SILAC), treated them with JB170 or Alisertib and subsequently compared their protein content to untreated cells by quantitative mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2020-09-28 | PXD017342 | Pride
Aurora-a Protac Mv4-11 Degronimid Jb170 
ProteomicsAurora-A interactome in MV4-11 cells
To determine interactors of Aurora-A, HA tagged Aurora-A was immunoprecipitated from MV4-11 cells stably expressing HA tagged Aurora-A wild type and compared to MV4-11 cells expressing empty vector.
ORGANISM(S): Homo sapiens (Human) 
2020-09-28 | PXD019684 | Pride
Aurora-a Mv4-11 
ProteomicsRNA Polymerase II interactome in presence or absence of SPT6 in U2OS cells
SPT6 is a histone chaperone that tightly binds RNA polymerase II (POL2) during transcription elongation. However, its primary role in POL2 transcription is uncertain. We used targeted protein degradation to rapidly deplete SPT6 in human cells and analyzed defects in POL2 behavior by a multi-omics ap...
ORGANISM(S): Homo sapiens (Human) 
2021-06-28 | PXD025243 | Pride
Aid Supt6h Spt6 Rna polymerase ii U2os Auxin 
GenomicsActivation and repression by oncogenic Myc shapes tumour-specific gene expression profiles
In mammalian cells, the Myc oncoprotein binds to thousands of promoters. During mitogenic stimulation of primary lymphocytes, Myc promotes an increase in expression of virtually all genes. In contrast, Myc-driven tumour cells differ from normal cells in expression of specific sets of up- and downreg...
ORGANISM(S): Homo sapiens 
2014-07-10 | E-GEOD-44672 | biostudies-arrayexpress
TranscriptomicsThe MYC-MIZ1 interaction defines tumor identity
Medulloblastoma (MB) is the most common malignant brain tumor. MB is a cerebellar tumor that occurs mostly in children between the ages of 3-7 years but also in adults. Human MBs are classified into four subgroups: Wingless (WNT), Sonic Hedgehog (SHH), Group 3 (G3) and G4, each of which with distinc...
ORGANISM(S): Mus musculus 
2016-01-21 | E-GEOD-64425 | biostudies-arrayexpress
GenomicsBIM is the primary mediator of MYC-induced apoptosis in multiple solid tissues
C-MYC (henceforth MYC) is one of the most frequently overexpressed oncogenes in human cancer and even modestly deregulated MYC expression can initiate ectopic proliferation in many post-mitotic, terminally differentiated cell types in vivo. Metazoan organisms have consequently evolved a number of me...
ORGANISM(S): Homo sapiens 
2015-05-18 | E-GEOD-59001 | biostudies-arrayexpress
TranscriptomicsDual regulation of Fbw7 function and oncogenic transformation by Usp28
Fbw7, the substrate recognition subunit of SCF(Fbw7) ubiquitin ligase, mediates turnover of multiple proto-oncoproteins and promotes its own degradation. Fbw7-mediated substrate degradation is antagonized by the Usp28 deubiquitinase. We now show, using knockout mice, that Usp28 preferentially deubiq...
ORGANISM(S): Mus musculus 
2015-05-01 | E-GEOD-59354 | biostudies-arrayexpress
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