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ProteomicsLabel-free shotgun proteomics of S. cerevisiae producing starch-like glucans
The synthesis of starch-like glucans can be reconstituted in S. cerevisiae using the genes from Arabidopsis thaliana: heterologous expression of SS1 to SS4, BE2, BE3, ISA1, and ISA2, together with a bacterial ADPglucose pyrophosphorylase gene for substrate provision in yeast strain 29, yielded insol...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-09-05 | PXD032241 | Pride
Lc-ms/ms Progenesisqi Yeast 
ProteomicsMass spectrometry of co-immunoprecipitation using tobacco Maturase K as bait
Chloroplast group IIA introns derive from bacterial ribozymes. Their splicing likely requires Maturase K (MatK), which has been largely inaccessible to functional analyses being itself a chloroplast intron-encoded protein. Here we used co-immunoprecipitation coupled with proteomics to identify prote...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD060055 | Pride
Lc-ms/ms Tobacco Co-immunoprecipitation Seedilings Chloroplasts 
ProteomicsSize-exclusion chromatography of soluble chloroplast proteins from Nicotiana tabacum (tobacco)
Maturase K (MatK) is the only splicing factor encoded in the chloroplast genomes of land plants. Here we used a transplastomic Nicotiana tabacum line, in which the plastidial MatK gene is C-terminally tagged with a 3x-HA tag, to conduct size-exclusion chromatography of soluble protein extracts enric...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD069946 | Pride
Maturase k Lc-ms/ms Tobacco Dia Size-exclusion chromatography Chloroplast 
ProteomicsMass spectrometry of co-immunoprecipitation using Arabidopsis MKIP1-YFP as bait
Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Here we used co-immunoprecipitation (IP) coupled with proteomics to identify proteins bound to MKIP1 in stably transformed Arabidopsis lines ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-10 | PXD060108 | Pride
Arabidopsis Maturase k Lc-ms/ms Co-immunoprecipitation Mkip1 
ProteomicsProteomics of Arabidopsis thaliana leaves after silencing of MKIP1 or uL4c
Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Inducible silencing of MKIP1 by estradiol-treatment results in pale newly emerged leaves. Here we analysed the proteome of these leaves from ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-10 | PXD067382 | Pride
Arabidopsis Lc-ms/ms Dia Ribosome Chloroplast 
ProteomicsProteomics of cassava storage roots before and after shoot pruning
Cassava (Manihot esculenta Crantz) is a food security crop for approximately 800 million people, primarily in developing economies. Cassava root yield is determined by the allocation of photoassimilated carbon within the plant, yet there is limited molecular and biochemical information about source-...
ORGANISM(S): Manihot esculenta (Cassava) (Jatropha manihot) 
2026-04-01 | PXD045690 | Pride
Manihot esculenta Storage roots Pruning Cassava 
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