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ProteomicsChikungunya virus phosphoproteomics
SILAC-labeled MRC-5 cells were seeded in 75-cm2 flasks 1 day before infection with CHIKV-LS3 [1] at MOI 5. One hour post infection (h p.i.), the inoculum was removed and replaced with SILAC DMEM containing 2% dialyzed FBS, 0.280 mM arginine, 0.384 mM lysine, 0.5 mM proline, 25 mM HEPES, 2 mM L-Gluta...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD009381 | Pride
Chikungunya virus chikv phosphoproteomics 
ProteomicsEBV latent nuclear antigen EBNA3A modulates IRF3-dependent IFNβ expression
Epstein-Barr virus (EBV) persistently infects over 90% of the human population and is the causative agent of infectious mononucleosis and human cancers. For the establishment of life-long infection, EBV tampers with the induction of type I interferon (IFN I)-dependent antiviral immunity in the host....
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD038572 | Pride
Immune evasion Viral protein Irf3 Proximity labeling Ifn i P300 Herpesvirus Viral immunology 
MetabolomicsAMPK activation induces RALDH+ tolerogenic dendritic cells by rewiring glucose and lipid metabolism.
Dendritic cell (DC) activation and function are underpinned by profound changes in cellular metabolism. Several studies indicate that the ability of DCs to promote tolerance is dependent on catabolic metabolism. Yet the contribution of AMP-activated kinase (AMPK), a central energy sensor promoting c...
2024-08-16 | MTBLS10647 | MetaboLights
ProteomicsProinsulin degradation and presentation of a proinsulin B-chain autoantigen involves ER-associated protein degradation (ERAD)-enzyme UBE2G2
Type 1 diabetes (T1D) is characterized by HLA class I-mediated presentation of autoantigens on the surface of pancreatic β-cells. Recognition of these autoantigens by CD8¬¬+ T cells results in the destruction of pancreatic β-cells and, consequently, insulin deficiency. Most epitopes presented at the...
ORGANISM(S): Homo sapiens (Human) 
2024-03-20 | PXD049996 | Pride
Ube2g2 Type 1 diabetes Insulin-derived peptides Proinsulin Hla-class i 
ProteomicsDevelopment of inhibitors, probes, and PROTAC provides a complete toolbox to study PARK7 in the living cell
The innovation of combined chemical tools, such as small molecule inhibitors, activity-based probes (ABPs), and proteolysis targeting chimeras (PROTACs) for a specific target, not only advances clinical drug discovery but also provides a chemical toolbox to study the diverse biological perspective o...
ORGANISM(S): Homo sapiens (Human) 
2024-06-16 | PXD047093 | Pride
Park7 Small molecule inhibitors Protacs Activity-based probes 
ProteomicsTemporal quantitative proteomics of Chikungunya virus infected cells
To better understand the complex CHIKV-host interplay we used stable isotope labeling with amino acids in cell culture (SILAC) and LC-MS/MS to define the temporal dynamics of the cellular response to infection. Using samples harvested at 8, 10 and 12 hours post infection, over 4700 proteins were ide...
ORGANISM(S): Cercopithecidae (Old World monkeys) 
2015-03-16 | PXD001330 | Pride
Silac Chikungunya virus Quantitative proteomics Lc-msms 
ProteomicsA library of novel Cancer Testis specific T cell receptors for T cell receptor gene therapy
Background Clinical success of T cell receptor (TCR) gene therapy has previously been demonstrated for NY-ESO-1 TCR gene therapy. To increase numbers of cancer patients that can be treated with TCR gene therapy we aimed to identify a novel set of high-affinity cancer specific TCRs targeting differen...
ORGANISM(S): Homo sapiens (Human) 
2023-01-23 | PXD038862 | Pride
T cell receptor Tcr gene therapy Mage Cd8+ t cells Cancer testis genes 
ProteomicsA T-cell receptor targeting RUNX1 frameshift mutations in acute myeloid leukemia
Runt-related transcription factor 1 (RUNX1) is a key regulator of hematopoietic differentiation. Disruption of RUNX1 in acute myeloid leukemia (AML) induces a maturation arrest and early myeloid progenitor phenotype. RUNX1 mutations occur in 10-15% of AML and are associated with poor prognosis. One-...
ORGANISM(S): Homo sapiens (Human) 
2025-07-31 | PXD062938 | Pride
Tcr-based immunotherapy Hla-peptidomics Runx1 Aml 
ProteomicsDevelopment of covalent probes to capture Legionella pneumophila effector enzymes
Upon infection of host cells Legionella pneumophila releases a multitude of effector enzymes into the hosts cytoplasm that hijack a plethora of cellular activities, including the hosts ubiquitination pathways. Effectors belonging to the SidE-family are involved in non-canonical serine phosphoribosyl...
ORGANISM(S): Homo sapiens (Human) Legionella pneumophila subsp. pneumophila Lp02 
2025-05-06 | PXD049797 | Pride
Legionella pneumophila Activity-based probe Ubiquitylation Adp-ribosylation Bacterial infection 
ProteomicsUsutu virus NS4A induces autophagy and is targeted by the selective autophagy receptor p62/SQSTM1 for degradation
Usutu virus (USUV) is an emerging orthoflavivirus, which mainly affects birds but in rare cases can cause severe neuroinvasive disease in humans. Due to the limited size of the orthoflavivirus genome the virus relies on the host machinery for replication. In addition, it must subvert the host antivi...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD057492 | Pride
Orthoflavivirus Autophagy Usutu virus Ns4a P62 
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