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An important step in the proteomic analysis is the optimal extraction and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. Cell lysis is important for proteomic profiling with an eventual aim to discover differently expressed proteins (DE...
ORGANISM(S): Homo sapiens (Human) 
2022-07-20 | PXD035202 | Pride
We evaluated the effect of the different solubilization techniques on the reproducibility and width of the chorionic proteome characterized using bottom-up proteomics: SDS-based solubilization coupled with in-gel protein digestion after applying self-named 1DE gel-concentration procedure (SDS-PAGE w...
ORGANISM(S): Homo sapiens (Human) 
2022-06-17 | PXD024759 | Pride
Background: Anopheles culicifacies is a rural vector of malaria in tropical and sub tropical South East Asian region. The salivary gland of the mosquito is the target for sporozoite interaction, blood feeding behavior, haemostasis and vector-parasite interactions. Malaria parasite matures inside th...
ORGANISM(S): Anopheles culicifacies A 
2016-09-13 | PXD003450 | Pride
The problem of the male reproductive system has both medical and social importance. It is highlighted that spermatozoa or seminal plasma are suitable samples for proteomic analysis of the male reproductive system. We suggested that the ejaculate could serve as one of the analytic specimens to determ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-23 | PXD046848 | Pride
Purpose: Prostate cancer is the most frequently diagnosed cancer in men and the third leading cause of cancer related deaths among men living in developed countries. Biomarkers that predict disease outcome at the time of initial diagnosis would aid management of the disease. Experimental design: Pr...
ORGANISM(S): Homo sapiens (Human) 
2015-09-14 | PXD000963 | Pride
Using an integrated model system for reproducible growth of biofilms, a JPIAMR-funded consortium of researchers* studied the expressed proteome of P. aeruginosa strain MPAO1 under i) planktonic growth, and ii) biofilm formation conditions. The model system included, as a first step, the sequencing a...
ORGANISM(S): Pseudomonas aeruginosa 
2021-09-09 | PXD017122 | Pride
Assembly of infectious influenza A viruses (IAV) is a complex process involving transport from the nucleus to the plasma membrane. Rab11A containing recycling endosomes have been identified as a platform for intracellular transport of viral RNA (vRNA). Using high spatiotemporal resolution light-shee...
ORGANISM(S): Homo sapiens (Human) Influenza A virus (A/California/07/2009(H1N1)) 
2020-01-14 | PXD016331 | Pride
Schistosome parasites lay up to a thousand eggs per day inside the veins of their mammalian hosts. The immature eggs deposited by females against endothelia of venules will embryonate within days. Approximately 30% of the eggs will migrate to the lumen of the intestine to continue the parasite lif...
ORGANISM(S): Candida albicans (Yeast) 
2019-09-26 | PXD012835 | Pride
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