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ProteomicsEvaluation of the technical reproducibility of serum proteome sample preparation methods to support clinical proteomics applications
Evaluation of the efficiency and reproducibility of sample prepration methods using iST-BCT kit from PreOmics or SMART Trypsin Kit (Thermo Fisher) with in-house Rapigest protocol in human serum samples prior to mass spectrometry analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-04-07 | PXD051002 | Pride
Serum  proteomics  biomarker  mass spectrometry  rapigest sf  ist-bct  smart digest trypsin 
UnknownAbsolute quantitation of disease protein biomarkers in a single LC-MS acquisition using apolipoprotein F as an example.
Not available
S-EPMC5608892 | biostudies-literature
ProteomicsDevelopment of an optimized LC-MS workflow for Host Cell Protein characterization to support upstream process development
Host cell proteins (HCPs) co-expressed during the production of biotherapeutics can affect the safety, efficacy and stability of the final product. As such, monitoring HCPs populations and amounts throughout the production and purification process are an essential part of the overall quality control...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-05-07 | PXD053035 | Pride
Process analytical technologies Host cell proteins Lc-ms Clarified cell culture fluid Absolute quantification Chinese hamster ovary Data-independent acquisition Hi3 quantification Bio-processing 
UnknownEvaluation of Serum Proteome Sample Preparation Methods to Support Clinical Proteomics Applications.
Not available
S-EPMC11546599 | biostudies-literature
ProteomicsRapid Highly-Efficient Digestion and Peptide Mapping of Adeno-Associated Viruses
[15:18] Toole, Estee Adeno-Associated Viruses (AAVs) comprise an area of rapidly growing interest due to their ability to act as a gene delivery vehicle in novel gene therapy strategies and vaccine development. Peptide mapping is a common technique in the biopharmaceutical industry to confirm ...
ORGANISM(S): Adeno-associated virus - 2 
2022-02-17 | PXD025971 | Pride
Viral therapies Pepsin Trypsin [15:18] toole Aav Peptide mapping Proteolysis Nanolc Estee gene therapy 
ProteomicsA comparative analysis of digestion methods for bile proteomics: the key to unlocking biliary biomarker potential in liver transplantation
Bile has gained increasing attention for its potential to reflect the overall health of the biliary system. Proteomic analysis of bile could deepen our understanding of the molecular pathophysiology of biliary disease and injury and provides a potential source of diagnostic biomarkers. With the emer...
ORGANISM(S): Homo sapiens (Human) 
2024-08-27 | PXD051062 | Pride
Human Bile Proteomics Liver Methods Ageing Biomarker 
ProteomicsTissue Microfibril Comparison - Structural and compositional diversity of fibrillin microfibrils in human tissues
Fibrillin microfibrils are large supramolecular ECM assemblies which play different functional roles in different tissues. We aim to compare the ultrastructure and biological composition of fibrillin microfibrils purified from human eye, skin and cultured dermal fibroblasts. This will allow us to un...
ORGANISM(S): Homo sapiens (Human) 
2018-02-16 | PXD008450 | Pride
Human Microfibril Afm Fibrillin Collagen Lc-ms/ms 
ProteomicsData-independent acquisition proteomics of cerebrospinal fluid implicates endoplasmic reticulum and inflammatory mechanisms in amyotrophic lateral sclerosis
Unbiased proteomics of human cerebrospinal fluid (CSF) has been used successfully to identify biomarkers of Amyotrophic Lateral Sclerosis (ALS). However, high abundance proteins mask the presence of lower abundance proteins that may hold valuable diagnostic and prognostic use. Developments in mass s...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD044274 | Pride
Amyotrophic lateral sclerosis Dia Csf Mass spectrometry Proteomics Biomarker 
ProteomicsAnopheles culicifacies species A salivary gland proteome
Background: Anopheles culicifacies is a rural vector of malaria in tropical and sub tropical South East Asian region. The salivary gland of the mosquito is the target for sporozoite interaction, blood feeding behavior, haemostasis and vector-parasite interactions. Malaria parasite matures inside th...
ORGANISM(S): Anopheles culicifacies A 
2016-09-13 | PXD003450 | Pride
Proteomics Anopheles culicifacies Salivary gland 1d electrophoresis Mass spectrometry Proteome 
ProteomicsValue of lipocalin 2 as a potential biomarker for bacterial meningitis
Objectives: Central nervous system (CNS) infections are common causes of morbidity and mortality worldwide. We aimed to discover protein biomarkers that could rapidly and accurately identify the likely cause of the infections, essential for clinical management and improving outcome. Methods: We app...
ORGANISM(S): Homo sapiens (Human) 
2020-08-20 | PXD020510 | Pride
Cns Csf Bacterial meningitis 
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