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ProteomicsPolypharmacological perturbation of the 14-3-3 adaptor protein interactome stimulates neurite outgrowth
Targeting protein-protein interactions (PPIs) is a promising, but under-exploited, approach in the development of drugs for many indications. 14-3-3 proteins are a family of adaptor molecules with ubiquitous and critical functions in dozens of cell signaling networks. 14-3-3s bind to hundreds of ‘cl...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-03-31 | PXD017317 | Pride
Regeneration Axon 14-3-3 Rap1 Polypharmacology 
ProteomicsStructural basis of ubiquitin ligase Nedd4-2 autoinhibition and regulation by calcium and 14-3-3 proteins
Nedd4-2 E3 ligase regulates Na+ homeostasis by ubiquitinating various channels and membrane transporters, including the epithelial sodium channel ENaC. In turn, Nedd4-2 dysregulation leads to various conditions, including electrolytic imbalance, respiratory distress, hypertension, and kidney disease...
ORGANISM(S): Homo sapiens (Human) 
2025-05-12 | PXD058899 | Pride
Saxs Calcium Protein-protein interaction H/d exchange Cryo-em Nedd4-2 14-3-3 proteins 
ProteomicsPIM1 phosphorylation of 14-3-3 ζ in vitro
We previously identified 14-3-3 ζ S64 as a substrate of PIM1 in an in vivo chemical genetic screen. To verify this phosphorylation event and determine whether S64 is the predominant phosphorylation site, we performed in vitro phosphorylation of 14-3-3 ζ by PIM1.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2021-11-25 | PXD023623 | Pride
Pim1 Protein phosphorylation Kinase 14-3-3 ζ 
ProteomicsStructural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16
Cyclin-dependent protein kinase 16 (CDK16) regulates both physiological and pathological processes, including autophagy, spermatogenesis and cancer. Unlike other CDKs, CDK16 is regulated by an unclear mechanism involving phosphorylated cyclin Y (CCNY) in complex with 14-3-3 proteins rather than CCNY...
ORGANISM(S): Escherichia coli 
2026-03-31 | PXD074528 | Pride
Structure Cdk16 Cyclin y/14-3-3 
Proteomics14-3-3 affinity capture to identify new targets of 14-3-3 signaling during dsRNA-stimulation in human keratinocytes.
We have combined two proteomic approaches, phosphoproteomics and 14-3-3 affinity capture, to examine the signaling pathways in which 14-3-3 proteins participate and to identify new targets of 14-3-3 signaling during dsRNA-stimulation.
ORGANISM(S): Homo sapiens (Human) 
2014-08-05 | PXD000677 | Pride
Human Itraq Hacat keratinocytes Lc-ms/ms 
ProteomicsLight-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism
NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key component of the auxin-dependent plant phototropic growth response. We report that NPH3 directly binds polyacidic phospholipids, required for plasma membrane association in darkness. We further demonstrate that blue light induces an immediate phosphorylati...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-10-12 | PXD028530 | Pride
Arabidopsis thaliana Etiolated seedling Blue-light dependent 14-3-3 interactors 
ProteomicsPhospho-docking protein 14-3-3 regulates microtubule-associated proteins in oocytes including the chromosomal passenger Borealin.
Global regulation of spindle-associated proteins is crucial in oocytes due to the absence of centrosomes and their very large cytoplasmic volume, but little is known about how this is achieved beyond involvement of the Ran-importin pathway. We previously uncovered a novel regulatory mechanism in Dr...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-05-04 | PXD030445 | Pride
Borealin. Oocytes Drosophila Microtubule-associated proteins 14-3-3 Quantitative proteomics Chromosomal passenger complex 
Proteomics14-3-3θ Phosphorylation at S232 Reduces its Interactome and Regulates Axonal Trafficking
14-3-3 proteins impact protein-protein interactions (PPIs) that regulate neuronal functions. The 14-3-3θ isoform is protective in Parkinson’s disease (PD) and Dementia with Lewy Bodies (DLB) models. Human PD and DLB brains show increased 14-3-3θ phosphorylation at S232. To understand the impact of 1...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-25 | PXD071112 | Pride
Axonal trafficking Affinity-purification mass spectrometry 14-3-3θ Parkinson’s disease Dementia with lewy bodies 
ProteomicsCaMKK2 Inactivation by cAMP-PKA Signaling and 14-3-3 Adaptor Proteins
The calcium-calmodulin (Ca2+-CaM) dependent protein kinase kinase-2 (CaMKK2) is activated by increases in intracellular Ca2+ and is a key regulator of cellular and wholebody energy metabolism. CaMKK2 inhibition protects against prostate cancer, hepatocellular carcinoma and metabolic derangements ind...
ORGANISM(S): Homo sapiens (Human) 
2020-11-06 | PXD020133 | Pride
Cyclic amp (camp) Calcium-calmodulin (ca2+-cam) dependent protein kinase kinase-2 (camkk2) Protein kinase a (pka) 14-3-3 adaptor proteins 
Proteomics14-3-3 protects AMPK phosphorylated TET2 from dephosphorylation by PP2A
Ten-eleven translocation-2 (TET2) is a member of the methylcytosine dioxygenase family of enzymes implicated in cancer and in aging due to its role as a global epigenetic modifier. TET2 has a large N-terminal domain followed by a catalytic C-terminal. Previous reports have demonstrated that the cata...
ORGANISM(S): Homo sapiens (Human) 
2020-01-13 | PXD016160 | Pride
5hmc Ampk Pp2a Tet2 Post-translational modification 14-3-3 
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