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ProteomicsValidation and application of a novel KLK6 activity based probe (IMP2352)
Pancreatic cancer has the lowest survival rate of all common cancers due to late diagnosis and limited treatment options. Serine hydrolases are known to mediate cancer progression and metastasis through initiation of signaling cascades and cleavage of extracellular matrix proteins, and the kallikrei...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD035111 | Pride
Abpp Klk Activity-based probe Kallikrein-related peptidase N-terminomics 
ProteomicsDual-Probe Activity-Based Protein Profiling Reveals Site-Specific Binding Dynamics of EGFR-Directed Drugs
Comparative, dose-dependent analysis of interactions between small molecule drugs and their targets, as well as off-targets, in complex proteomes is crucial for selecting optimal drug candidates. The affinity of small molecules for targeted proteins is largely dictated by interactions between amino ...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD045864 | Pride
Chemical proteomics  activity-based protein profiling  site-specific  concentration-dependent analysis  dual-probe comparison. 
ProteomicsInvestigating B12 binding proteins in bacteria using a B12-activity-based-probe, Hv_B12ABP addendum
Elucidating the mechanistic underpinnings of a cobalamin activity-based probe in microbial cell growth, gene regulation, and enzyme activity
ORGANISM(S): Haloferax volcanii (Halobacterium volcanii) 
2018-10-22 | PXD008595 | Pride
Activity-based-probe B12 E. coli 
ProteomicsDiscovery of a potent and selective covalent inhibitor and activity-based probe for UCHL1, with anti-fibrotic activity
Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) is a deubiquitinating enzyme (DUB) that is very highly expressed in human brain. UCHL1 has been proposed as a potential therapeutic target in neurodegeneration, cancer, and liver and lung fibrosis, however bona fide molecular functions of UCHL1 are yet...
ORGANISM(S): Homo sapiens (Human) 
2020-11-12 | PXD015825 | Pride
Activity based-probe profiling Human Uchl1 Chemical proteomics Deubiquitination 
ProteomicsZNFX1 activity-based probe crosslinking mass spectrometry
The aim of this experiment was to identify the catalytic cysteine in the antiviral E3 ligase ZNFX1 using activity-based probe (ABP)–enabled cross-linking mass spectrometry (XL-MS). A biotinylated E2~ubiquitin activity-based probe containing a cysteine-electrophile trap was used to covalently capture...
ORGANISM(S): Homo sapiens (Human) 
2026-05-22 | PXD073419 | Pride
ProteomicsElectrophile scanning by chemical prote-omics reveals a potent pan-active DUB probe for investigation of deubiquitinase activity in live cells
Deubiquitinases (DUBs) are proteases that hydrolyze isopeptide bonds linking ubiquitin to protein substrates, which can lead to reduced substrate degradation through the ubiquitin proteasome system. Deregulation of DUB activity has been im-plicated in many disease states, including cancer, neurodege...
ORGANISM(S): Homo sapiens (Human) 
2024-01-03 | PXD035417 | Pride
Ubiquitin-activity base probe Deubiquitinase Chemical probe Activity-based probe Electrophilic war-head Ubiquitin Dub Enzyme targeting Myc-deregulated cancer 
Proteomicsproteomics analysis of SDE2UBL base activity based probe immunoprecipitation
SDE2, a highly conserved and essential gene required for genome maintenance, coordinates essential processes ranging from pre-mRNA splicing and ribosome biogenesis to DNA repair and replication stress response. It is expressed as a full-length precursor (SDE2FL,) bearing an N-terminal ULD, which is ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-24 | PXD064155 | Pride
Ubiquitin-like fusion proteins Sde2 Activity-based probe Proteolysis Immunoprecipitation 
ProteomicsSmall-Molecule Activity-Based Probe for Monitoring Ubiquitin 2 C‑Terminal Hydrolase (UCHL1) Activity in Live Cells and Zebrafish 3 Embryos
Many reagents have emerged to study the function of specific enzymes in vitro. On the other hand, target specific reagents are scarce or need improvement, allowing investigations of the function of individual enzymes in their native cellular context. Here we report the development of a target-select...
ORGANISM(S): Homo sapiens (Human) 
2020-09-18 | PXD021557 | Pride
Orbitrap Human 
ProteomicsCP-24,879: an activity-based probe of fatty acid desaturases and related diiron lipid oxidases
: CP-24,879 inhibits fatty acid desaturase 2 (FADS2), a diiron lipid oxidase necessary for polyunsaturated fatty acid (PUFA) biosynthesis, with exceptional cellular potency (EC50 15 nM) despite its fragment-like structure. Here we demonstrate CP-24,879 is activated in cells to a reactive intermedia...
ORGANISM(S): Homo sapiens (Human) 
2026-09-28 | PXD072626 | Pride
Diiron lipid oxidases Lipid metabolism Mechanism of action Chemical proteomics Activity-based probe 
ProteomicsActivity-based proteomic profiling (ABPP) employing a serine hydrolase-directed probe to identify changes in activity profiles of lipid hydrolase in lung tumors
Lung tumors, as well as normal tumor-adjacent (NTA) tissue of non-small cell lung cancer (NSCLC) patients, were collected and subjected to labeling with a serine hydrolase-directed activity-based probe (ABP) immediately after excision. Labeling with the serine hydrolase-directed ABP allows for the d...
ORGANISM(S): Homo sapiens (Human) 
2023-11-27 | PXD046934 | Pride
Abpp Lipid hydrolysis Serine hydrolase Nsclc Lipid hydrolase Lung tumor Activity-based proteomic profiling Lipase 
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