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ProteomicsProtein target identification of CL16 in HCT116 cells by an alkyne probe, CL16-alkyne
By coupling activity-based protein profiling (ABPP) with covalent ligand screening experiment, we have identified a cysteine-reactive covalent ligand, CL16, which can bind strongly and selectively with RhoA in vitro. In this experiment, we investigated the protein targets of CL16 in colorectal cance...
ORGANISM(S): Homo sapiens (Human) 
2025-08-30 | PXD053041 | Pride
Lc-ms/ms experiment on hct116 cells probed by cl16-alkyne to investigate protein targets of cl16 
ProteomicsAlkyne-probe pull-down in LAPC4-CR cells
LAPC4-CR cells were treated with alkyne-probe or DMSO for 2 h in triplicate, lysed and conjugated with biotin-picolyl-azide. Biotinylated proteins were pulled down with neutravidin agarose. After the pulldown, the neutravidin agarose beads were treated with trypsin, and the tryptic peptides were lab...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-30 | MSV000093542 | MassIVE
ProteomicsProteome analysis of i-motif DNA binding proteins
Non-canonical DNA structures such as G-quadruplex (G4) and i-motif (iM) are formed at the guanine- and cytosine-rich sequences, respectively, and prohibit DNA replication and transcription. The formation and resolution of these non-canonical structures are therefore required to be dynamically regula...
ORGANISM(S): Homo Sapiens (human) 
2024-10-15 | PXD051188 | JPOST Repository
GenomicsComparative Analysis of Alkyne- and Desthiobiotinylated Photoaffinity Probes for Chemotranscriptomic Profiling
Comparative Analysis of Alkyne- and Desthiobiotinylated Photoaffinity Probes for Chemotranscriptomic Profiling
PRJNA1416391 | ENA
TranscriptomicsComparative Analysis of Alkyne- and Desthiobiotinylated Photoaffinity Probes for Chemotranscriptomic Profiling
Understanding small molecule-RNA interactions is a crucial part in drug development and fundamental biology. Chemotranscriptomic profiling is emerging as a powerful platform to interrogate interactions of small molecules with entire transcriptomes. This technique relies on photoaffinity probes that ...
ORGANISM(S): Escherichia coli 
2026-03-18 | GSE318057 | GEO
ProteomicsValidation of PG-DA probe in mammalian cell and brain lysates
Here we aim to characterize the 1-palmitoyl-glycerol diazirine alkyne (PGDA) probe using chemical proteomics approaches in mammalian cell and mouse brain lysates.
ORGANISM(S): Mus musculus (Mouse) 
2025-06-24 | PXD051332 | Pride
Chemoproteomics Lipid probe Mag 
ProteomicsIn vivo labelling of sediments from Kamchatka Peninsula springs with FP-alkyne
This experiment aims on the identification of serine hydrolases from a complex thermophile community that live in a hot vent in Kamchatka Peninsula based on in vivo labelling with FP-alkyne directly in the hot spring and subsequent analysis using metagenomics/metaproteomics. To this end, sediment sa...
ORGANISM(S): hot springs metagenome 
2024-06-03 | PXD025833 | Pride
Abpp Kamtchatka Fp Fluorophosphonate Metagenome 
ProteomicsProfiling of Methylglyoxal Blood Metabolism and Advanced Glycation End-Product Proteome using a Chemical Probe
Methylglyoxal (MG) is a toxic byproduct of the glycolytic pathway and is quantitatively the most important precursor to advanced glycation end-products (AGEs). Insight into which proteins and in particular their individual modification sites are central to understand the involvement of MG and AGE in...
ORGANISM(S): Homo sapiens (Human) 
2019-07-31 | PXD011314 | Pride
Human Erythrocytes Plasma Methylglyoxal 
ProteomicsAn alkyne-containing isoprenoid analogue based on a farnesyl diphosphate scaffold is a biologically functional universal probe for proteomic analysis
Prenylation consists of the modification of proteins with either farnesyl diphosphate (FPP) or geranylgeranyl diphosphate (GGPP) at a cysteine near the C-terminus of target proteins to generate thioether-linked lipidated proteins. Given the widespread involvement of prenylation in protein localizati...
ORGANISM(S): Homo sapiens (Human) 
2025-04-08 | PXD055402 | Pride
Prenylation Prenylomics Chemical probes Post-translational modifications 
ProteomicsPronucleotide probe for in vivo imaging of protein AMPylation.
Protein AMPylation is a prevalent posttranslational modification in human cells involved in the regulation of unfolded protein response and neural development. Here we present a novel pro-N6azA probe suitable for in vivo imaging and chemical proteomics profilling of AMPylated proteins. The pro-N6azA...
ORGANISM(S): Homo sapiens (Human) 
2019-12-10 | PXD016011 | Pride
Chemical proteomics Ampylation 
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