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TranscriptomicsRNA immunoprecipitation to identify RHA-binding transcripts in HEK293 cells
Given that RHA regulates translation by binding a PCE located at the 5’ UTR of the target transcripts a genome-wide screen was performed to identify mRNAs that bind RHA in vivo. The results from four experiments with samples obtained in four independent RNA immunoprecipitations identified 375 transc...
ORGANISM(S): Homo sapiens 
2010-08-19 | E-GEOD-23688 | biostudies-arrayexpress
ProteomicsAnti-Flag AP-MS of cytoplasmic 3×Flag-importin α1 complexes in HeLa S3 cells
To identify binding partners of importin α1 in the cytoplasm, co-immunoprecipitation using an anti-Flag antibody in the cytoplasmic fraction of 3×Flag–importin α1-inducible HeLaS3 cells.
ORGANISM(S): Homo sapiens (Human) 
2026-04-22 | PXD076745 | Pride
Nuclear localization signal Helas3 Importin Nls 
TranscriptomicsThe RPB2 flap loop of human RNA polymerase II is dispensable for transcription initiation and elongation.
Deletion of the flap loop in the Rp2 subunit of human RNAPII did not alter the ability of human RNAPII to initiate transcription or elongate the initiated transcripts in vivo and in vitro, and was also dispensable for elongation factor-mediated enhancement and inhibition of transcript elongation in ...
ORGANISM(S): Homo sapiens 
2011-06-24 | E-GEOD-29734 | biostudies-arrayexpress
ProteomicsFLAG-PHF3 interactome in HEK293 cells
FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026292 | Pride
Human Anti-flag immunoprecipitation Nanolc-ms analysis On-bead digest 
ProteomicsRBM39 organizes an m⁶A-dependent silencing complex to degrade Tat mRNA and maintain HIV-1 latency
To further elucidate the interaction between RBM39 and its partern proteins, Flag-tagged RBM39 were transfected into HEK293T cells. After transfection, RBM39 and RBM39-bound proteins were enriched using anti-Flag beads. Subsequently, the enriched proteins were separated by SDS-PAGE and analyzed by L...
ORGANISM(S): Homo sapiens (Human) 
2025-10-31 | PXD064858 | Pride
Lc/ms Hek293t Rbm39 Co-ip 
ProteomicsImmunoprecipitation was performed with HCT116 cells overexpressing 3xFLAG-SET/TAF-Ib with anti-FLAG antibodies
Immunoprecipitation was performed with HCT116 cells overexpressing 3xFLAG-SET/TAF-Ib with anti-FLAG antibodies. The immunoprecitates were separated by SDS-PAGE and putative interaction partners of SET/TAF-Ib were identified with mass spectrometry.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-12-13 | MSV000090898 | MassIVE
ProteomicsImmunoprecipitation coupled to LC-MS/MS analysis of Flag and Flag-DJ-1 complexes in HEK293T cells
This dataset contains raw mass spectrometry data and associated protein and peptide identification results from anti-Flag immunoprecipitation coupled to LC-MS/MS analysis of Flag and Flag-DJ-1 complexes in HEK293T cells. The dataset was generated to identify candidate DJ-1-associated proteins and is...
ORGANISM(S): Homo sapiens (Human) 
2026-07-24 | PXD081552 | Pride
Affinity purification-mass spectrometry Hek293t cells Dj-1 Immunoprecipitation Park7 Protein-protein interactions Lc-ms/ms 
ProteomicsAnalysis of proteins immunoprecipitated with anti-FLAG antibodies from Leishmania donovani promastigotes expressing Cdc45-FLAG or carrying empty vector
This study was carried out to identify the interacting partners of Leishmania donovani Cdc45, a protein that is conserved in eukaryotic DNA replication. Accordingly, Cdc45 was expressed in Leishmania promastigotes with a C terminal FLAG tag and whole cell lysates were isolated from these Ld1S transf...
ORGANISM(S): Leishmania donovani 
2025-10-27 | PXD064001 | Pride
Lc-ms Leishmania donovani Immunoprecipitation Cdc45-flag 
TranscriptomicsGenome-wide characterization of PhoB binding profile in Escherichia coli (ChIP-chip data)
Chromatin immunoprecipitation was combined with high-density tiling array (ChIP-chip) and gene expression microarray to reveal the adaptive responses of Escherichia coli to phosphate starvation. The first sketch of the genome-wide distribution of PhoB binding profile was unveiled and 43 regions were...
ORGANISM(S): Escherichia coli 
2012-10-21 | E-GEOD-21856 | biostudies-arrayexpress
TranscriptomicsCo-immunoprecipitation of RNAs with RNA helicase A (RHA)
Given that RHA regulates translation by binding a PCE located at the 5’ UTR of the target transcripts a genome-wide screen was performed to identify mRNAs that bind RHA in vivo. The results from duplicate experiments with samples obtained in two independent RNA immunoprecipitations identified 407 tr...
ORGANISM(S): Chlorocebus aethiops 
2008-12-19 | E-GEOD-14056 | biostudies-arrayexpress
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