Sort   by:  
 Page size 
UnknownCalcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue.
Not available
S-EPMC4868260 | biostudies-literature
ProteomicsTime-Resolved Analysis of Protein-Protein Ensembles using a Destabilizing Domain (TRAPPED) to resolve dynamic interactions of SARS-CoV-2 nsp15
Cellular protein-protein interactions are dynamic and can change over time. Current interactomics methods capture steady-state information but lack the ability to differentiate when the interactions occur. This study developed a post-translational method for synchronizing protein accumulation that a...
ORGANISM(S): Homo sapiens (Human) 
2025-09-29 | PXD063935 | Pride
Dihydrofolate reductase Proteomics Affinity purification-mass spectrometry Interactomics Destabilizing domain Virus-host protein interactions 
MetabolomicsAntiviral metabolite 3'-Deoxy-3',4'-didehydro-cytidine is detectable in serum and identifies acute viral infections including COVID-19

BACKGROUND: There is a critical need for rapid viral infection diagnostics to enable prompt case identification in pandemic settings and support targeted antimicrobial prescribing.

METHODS: Using untargeted high-resolution liquid chromatography coupled with...

2022-02-10 | MTBLS718 | MetaboLights
ProteomicsIdentifying candidate protein markers of acute kidney injury in acute decompensated heart failure using sheep kidney cortex
A quarter of patients with acute decompensated heart failure (ADHF) experience acute kidney injury (AKI), an abrupt loss of kidney function that doubles the risk of death at one-year. There is a critical need to identify early markers for AKI in ADHF, however, no protein candidates have been validat...
ORGANISM(S): Ovis aries 
2022-01-19 | PXD028983 | Pride
Adhf Kidney Sheep Swath-ms Tripletof Acute kidney injury 
MetabolomicsHuman Gut Microbiota from Autism Spectrum Disorder Promote Behavioral Symptoms in Mice
Autism spectrum disorder (ASD) manifests as alterations in complex human behaviors including social communication and stereotypies. In addition to genetic risks, the gut microbiome differs between typically-developing (TD) and ASD individuals, though it remains unclear whether the microbiome contrib...
2019-07-04 | MTBLS726 | MetaboLights
ProteomicsMitochondrial Membrane Potential Regulates Nuclear Gene Expression in Macrophages Exposed to PGE2
Metabolic engagement is intrinsic to immune cell function. Prostaglandin E2 (PGE2) has been shown to modulate macrophage activation, yet how PGE2 might affect metabolism is unclear. Here we show that PGE2 causes mitochondrial membrane potential (Δψm) to dissipate in interleukin-4 activated macrophag...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-08 | PXD010997 | Pride
Macrophage Prostaglandin e2 Camp Got1 Malate-aspartate shuttle Immunometabolism Mitochondrial membrane potential Etv1 Alternative activation Il-4 
ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030796 | Pride
Speed Plasma Kidney Sheep Swath-ms Tripletof S-trap 
ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030797 | Pride
Speed Plasma Kidney Sheep Tripletof S-trap Dda 
TranscriptomicsGene expression and genotype in normal heart
Genome-wide association studies have identified a small region at chromosome 9p21.3 strongly associated with coronary heart disease risk. The region contains no protein-coding genes and the mechanism underlying its association with heart disease is unknown. We investigated associations between rs133...
ORGANISM(S): Homo sapiens 
2012-07-03 | E-GEOD-22253 | biostudies-arrayexpress
MetabolomicsDirect on-swab metabolic profiling of vaginal microbiome:host interactions during pregnancy and preterm birth

The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of...

2021-09-23 | MTBLS717 | MetaboLights
Sort   by:  
 Page size