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ProteomicsNuclear hormone-sensitive lipase controls adipocyte function
Hormone-sensitive lipase (HSL) is a neutral lipase which hydrolyses fat stored as triacylglycerols in adipocyte lipid droplets. Its activity is under the control of hormones and neurotransmitters through protein kinase (PK)-mediated phosphorylation. Here we show that HSL is expressed in the nucleus ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-30 | PXD043860 | Pride
Obesity Adipocyte Hormone-sensitive lipase Protein-protein interaction Adipose tissue 
ProteomicsAnalysis of the CBL and CBLB signalosomes in primary T cells
T-cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin-protein ligases CBL and CBLB. Here we combine mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling...
ORGANISM(S): Mus musculus (Mouse) 
2016-12-14 | PXD004130 | Pride
Ap-ms Cblb Primary t cell Cbl Tcr Interactome Signalosome 
ProteomicsAnalysis of the TCR signalosome in primary T cells
T-cell receptor (TCR) signaling is essential for the function of T cells. Here we combine mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the composition and dynamics of the signaling complexes that assemble around 15 nodes of the TCR signaling cascade o...
ORGANISM(S): Mus musculus (Mouse) 
2019-09-26 | PXD007660 | Pride
Ap-ms Tcr Primary t cell Interactome Signalosome 
ProteomicsQuantification of nuclear factors in hMADS adipocytes
We performed unbiased quantification of nuclear factors in hMADS adipocytes. To do so, we performed subcellular fractionnation of differentiated hMADS cell followed by mass spectrometry analysis of nuclear extracts.
ORGANISM(S): Homo sapiens (Human) 
2026-01-30 | PXD060011 | Pride
Nucleus Adipocytes Human 
ProteomicsAnalysis of the Vav1 interactome in primary T cells
In this study, we combined mouse genetics and quantitative proteomics to characterize in a comprehensive manner the VAV1 interactome. A line of knock-in mouse expressing endogenous VAV1 molecules with a genetic tag permitting affinity purification was generated and combined with affinity purificatio...
ORGANISM(S): Mus musculus (Mouse) 
2018-06-26 | PXD004217 | Pride
Ap-ms Primary t cell Tcr Vav1 Interactome Signalosome 
ProteomicsProteomic analysis of the extracellular and intracellular role of IL-33 in primary human endothelial cells (IL-33 knockdown by siRNA)
IL-33 is a nuclear cytokine from the IL-1 family that plays important roles in health and disease. Under healthy conditions, IL-33 is constitutively expressed to high levels in the nucleus of producing cells in various human and mouse tissues. The extracellular function of IL-33 cytokine has been we...
ORGANISM(S): Homo sapiens (Human) 
2016-10-12 | PXD004141 | Pride
Cytokine Inflammation Endothelial cells Human Fractionation Shotgun Il-33 Huvec 
ProteomicsProteome of mouse primary CD4 T cells
We analyzed the total proteome of CD4+ T cells isolated from WT mice, either non stimulated or at 5 different time points of stimulation with anti-CD3 and anti-CD4 antibodies (30s; 120s; 300s; 600s)
ORGANISM(S): Mus musculus (Mouse) 
2019-09-27 | PXD012826 | Pride
Primary cd4 t cell Whole cell lysate Proteome 
ProteomicsImpact of hormone-sensitive lipase depletion on adipocyte secretome
Hormone-sensitive lipase (HSL) is an enzyme involved in the breakdown of triacylglycerols into fatty acids, a catabolic pathway called lipolysis. Out of all mammalian cells, adipocytes express the highest levels of HSL. To explore the roles of HSL beyond adipocyte lipolysis, we performed transcript...
ORGANISM(S): Homo sapiens (Human) 
2026-01-30 | PXD029261 | Pride
Obesity Adipocyte Hormone-sensitive lipase Extracellular matrix 
ProteomicsQuest for missing proteins in the human spermatozoa: an update
Here, we report work as part of C-HPP initiatives on chromosomes 14 (France) and 2 (Switzerland) aiming to identify missing proteins in ejaculated human spermatozoa using multiple MS-based and antibodies-based validation approaches
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080826 | MassIVE
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