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ProteomicsDNA damage-induced chromatome analysis in yeast reveals recruitment of Ubiquitin ligases for efficient DNA repairtest1
Eukaryotic cells package their genomes around histone octamers. In response to DNA damage checkpoint kinase-induced core histone degradation leads to a 20-40% reduction in nucleosome density in yeast. To gain insights into this process we report the first comprehensive proteomic analysis of yeast ch...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-02-02 | PXD022369 | Pride
Hmgb1/nhp6 Dna dynamics S. cerevisiae Teschromatome Chromatin proteomics Ino80 Ubiquitin ligases Proteasome Zeocin Homologous recombination Dna damage response Histone degradation 
ProteomicsChromatome of AR and SUMO2/3 idenitified with RIME
We identifyied AR and SUMO2/3 chromatomes in castration-resistant prostate cancer cells (VCaP) and studied the impact on SUMOylation inhibition (SUMOi)on them utilizing small molecule inhibitor ML792. Chromatome members were identifyied with Rapid Immunoprecipitation Mass spectrometry of Endogenous ...
ORGANISM(S): Homo sapiens (Human) 
2024-08-08 | PXD039179 | Pride
Ar Sumoylation Rime Chromatome Bruker timstof pro 
ProteomicsComprehensive chromatome profiling identifies metabolic enzymes on chromatin in healthy and cancer cells
we have performed a subcellular fractionation protocol, which enriches the chromatin-bound proteins across a panel of tissue types, both for cancerous and untransformed cells. Indeed we identify both metabolic enzymes which are consistently moonlighting on chromatin, while others are tissue and canc...
ORGANISM(S): Homo sapiens (Human) 
2025-11-13 | PXD047504 | Pride
Chromatome  cancer  chromatin 
ProteomicsRegulatory phosphosite on Mec1 and control of RNAP11 and PIII during replication stress
Yeast sensor checkpoint kinase Mec1 is phosphorylated at Ser1991 upon HU replication stress. The phospho-accepter mutant (mec1-S1991A) confers HU sensitivity and synthetic sickness with the mutants that aggravate replication and transcription collision. To understand how the entire chromatin proteom...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-28 | PXD027337 | Pride
Lc-msms Mec1 Yeast Chromatome Phosphoproteomics Replication stress 
Unknown(Re)defining the human chromatome: an integrated meta-analysis of localization, function, abundance, physical properties, and domain composition of chromatin proteins.
Not available
S-EPMC12802929 | biostudies-literature
ProteomicsChromatomes of GR and SUMO2/3 identified with RIME
We identified GR and SUMO2/3 chromatomes in B-cell acute leukemia cells (NALM6) and studied the impact of SUMOylation inhibition (SUMOi) on them utilizing small molecule inhibitor ML792. Chromatome members were identified with Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME)...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD051304 | Pride
Human Cancer Immunoprecipitation Mass spectrometry Chromatin 
ProteomicsA quantitative proteomic based approach to study the genistein induced epigenetic reprogramming in MDA-MB231 cells
Epidemiological data indicate that consumption of soy-based food significantly reduces the cancer risk in human through interaction with estrogen receptors and the ‘phytoestrogen’ genistein present in the soy is responsible for this chemopreventive activity. The epigenome regulatory effect of genist...
ORGANISM(S): Homo sapiens (Human) 
2018-07-05 | PXD006178 | Pride
Epigenome Epigenetics Dna repair Tmt Genistein Chromatome Heterochromatin Epigenetic reprogram Euchromatin Quantitative proteomics Chromatin 
ProteomicsComprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components
The establishment of cellular identity is driven by transcriptional and epigenetic regulators of the chromatin proteome - the chromatome. Comprehensive analyses of the chromatome composition and dynamics can therefore greatly improve our understanding of gene regulatory mechanisms. Here, we develope...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-02-27 | PXD034448 | Pride
Naive Dia Pluripotency Mass spectrometry Stem cells Formative Chromatin proteomics Primed 
ProteomicsSMARCAD1 and TOPBP1 contribute to heterochromatin maintenance at the transition from the 2C-like to the pluripotent state
Chromocenters are established after the 2-cell (2C) stage during mouse embryonic development, but the factors that mediate chromocenter formation remain largely unknown. To identify regulators of 2C heterochromatin establishment, we generated an inducible system to convert embryonic stem cells (ESCs...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-18 | PXD019703 | Pride
Lc-msms Esc Chromatome 
UnknownChromatin capture links the metabolic enzyme AHCY to stem cell proliferation.
Not available
S-EPMC6402848 | biostudies-literature
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