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UnknownIdentification of candidate substrates for the Golgi Tul1 E3 ligase using quantitative diGly proteomics in yeast.
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S-EPMC4223478 | biostudies-literature
ProteomicsGlobal and diGLY enriched proteomes of RanBPM depleted HeLa cells and RanBPM APMS
The human CTLH complex is a newly discovered multi-subunit E3 ligase. At present, only a few of its ubiquitination targets are known. Here, we use proteomic techniques in RanBPM-depleted HeLa cells to identify ubiquitination substrates of the CTLH complex. First, global proteomics determined protein...
ORGANISM(S): Homo sapiens (Human) 
2021-08-13 | PXD024872 | Pride
Human Hela Ctlh complex 
ProteomicsLysosomal damage proteomics in HeLa cells
To identify ubiquitylation targets following lysosomal damage in HeLa cells treated with LLOMe we performed quantitative ubiquitin-remmnant (diGly) profilig coupled to mass spectrometry (MS). In addition, we performed APEX2-based proximity biotinylation followed by MS analysis to identify proximity ...
ORGANISM(S): Homo sapiens (Human) 
2022-06-23 | PXD029926 | Pride
Apex2 Lysosome Proxiity biotinylation Llome Digly proteomics Ubiquitin Cnn2 
ProteomicsData-independent acquisition method for ubiquitinome analysis reveals non-degrative regulation of circadian biology
Protein ubiquitination is involved in virtually all cellular processes. Enrichment strategies employing antibodies targeting ubiquitin-derived diGly remnants combined with mass spectrometry (MS) have enabled investigations of ubiquitin signaling at a large scale. However, so far the power of data in...
ORGANISM(S): Homo sapiens (Human) 
2020-11-20 | PXD019854 | Pride
Circadian biology Dia 
ProteomicsImprovement of ubiquitination site detection by Orbitrap mass spectrometry.
We describe a modified workflow to enrich for and detect diGly peptides originating from ubiquitinated proteins using immunopurification. Using a combination of several relatively simple modifications in the sample preparation and mass spectrometric detection protocols, we now routinely detect over ...
ORGANISM(S): Homo sapiens (Human) 
2017-11-14 | PXD007101 | Pride
Ubiquitinome Silac Hela Ubiquitination U2os Ubiquitin Orbitrap lumos Digly peptides Mouse brain tissue 
ProteomicsProteome and ubiquitinome changes in Huntington’s disease
Huntington’s disease is caused by a polyglutamine repeat expansion at the N-terminus of the huntingtin protein which affects the function and folding of the protein, and results in intracellular protein aggregates. Here, we examined whether this mutation leads to altered ubiquitination of huntingtin...
ORGANISM(S): Mus musculus (Mouse) 
2019-06-05 | PXD010161 | Pride
Ubiquitination Digly Ptms Brain Lfq Mouse Huntington 
ProteomicsIdentification of Candidate Substrates for the Golgi Tul1 E3 Ligase Using Quantitative diGly Proteomics in Yeast
Maintenance of protein homeostasis is essential for cellular survival. Central to this regulation are mechanisms of protein quality control in which misfolded proteins are recognized and degraded by the ubiquitin-proteasome system. One well-studied protein quality control pathway requires ER residen...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-11-12 | PXD000918 | Pride
Protein homeostasis Ubiquitination Silac 
ProteomicsdiGly ubiquitin remnant profiling upon TFEB overexpression
We performed proteomic analysis of di-Gly peptides to identify which proteins are ubiquitinated in cells overexpressing TFEB compared to non-expressing cells.
ORGANISM(S): Homo sapiens (Human) 
2025-11-18 | PXD065497 | Pride
Ubiquitin Digly Tfeb Hela 
ProteomicsUSP32 regulates non-proteolytic LAMTOR1 ubiquitination and Ragulator function
Ubiquitination is among the most prevalent post-translational modifications regulating a great number of cellular functions, and defects in such processes contribute to many diseases. Deubiquitinating enzymes (DUBs) are essential to control the precise outcome of ubiquitin signals. The ubiquitin-spe...
ORGANISM(S): Homo sapiens (Human) 
2022-12-16 | PXD024211 | Pride
Ubiquitin Digly Lysosome 
ProteomicsPancreatic ductal adenocarcinoma (PDAC) ubiquitination profiling reveals specific prognostic and theranostic markers.
The project was aimed at identifying diGlycine (diGly) conjugated peptides obtained from sixty PDACs' patients' derived xenografs (PDXs) then to identify potential diGLy profiles that could be used as prognosis markers of the aggressiveness of the tumour and overall survival of patients as well as t...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD030011 | Pride
Theranostic markers. Ubiquitinome Digly peptide profiling Prognostic markers Lc-msms Pdac 
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