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ProteomicsGlobal quantitative MS proteome analysis of in vitro activated murine primary B cells of Dis3G766R/+ and Dis3+/+ genotypes
DIS3 is a ribonucleolytic subunit of the exosome complex responsible for RNA degradation in the nucleus, predominantly affecting steady state levels of non-coding transcripts. The effect of the mild mutant Dis3 G766R allele, clinically related t multiple myeloma, in a heterozygous state on protein-c...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-13 | PXD050438 | Pride
Mouse Dis3g766r/+ and dis3+/+ genotypes Lps and il-4 activation B cells 
ProteomicsEfficient globin production during terminal erythropoiesis depends on the cooperative action of TENT5C poly(A) polymerase and LARP4B in mice
To identify erythroid-specific TENT5C interactors, we employed a modified TurboID proximity biotinylation assay followed by MS/MS analysis of fetal liver erythroblasts differentiated ex vivo. We took advantage of Tent5c-EGFP knock-in mouse line and employed lentiviral vector expressing nanobody anti...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-29 | PXD072661 | Pride
Cytoplasmic polyadenylation Tent5c Erythropoiesis Interactome 
ProteomicsThe non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma
FAM46C is one of the most frequently mutated genes in multiple myeloma (MM). Here, using a combination of in vitro and in vivo approaches, we demonstrate that FAM46C encodes an active non-canonical poly(A) polymerase which enhances mRNA stability and gene expression. Reintroduction of active FAM46C ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-09 | PXD006790 | Pride
Lc-msms Fam46c Anemia Mouse B lymphocytes Polyadenylation Multiple myeloma 
ProteomicsDegron models: a toolbox for rapid in vivo depletion of essential proteins regulating mRNA metabolism
Due to their essentiality, studying proteins involved in fundamental processes in vivo is challenging. PROTAC-based systems offer time-controlled protein depletion, but their characterization in vivo remains limited. Here, with an efficient direct zygote editing protocol, we generated degron-tag mod...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-26 | PXD069282 | Pride
Tmt Degron-tag Liver Mouse 
ProteomicsTENT5 cytoplasmic non-canonical poly(A) polymerases regulate the innate immune response in animals
Innate immunity, the first line of host defense against pathogens, is tightly regulated both transcriptionally and post-transcriptionally. Here, through global transcriptome and proteome analyses in Caenorhabditis elegans, we uncover a modulation of the expression of secreted innate immunity effecto...
ORGANISM(S): Caenorhabditis elegans 
2022-05-31 | PXD023238 | Pride
Innate immunity Cytoplasmic polyadenylation Non-canonical polyadenylation Tent5 Direct rna sequencing Terminal nucleotidyltransferase C. elegans innate immunity 
ProteomicsRNA-bound proteome of Mycobacterium bovis
The dataset accompanying a publication describing the RNA degradosome components in Mycobacterium tuberculosis (Mtb). The RNA-bound proteome was determined by affinity purification of protein-RNA complexes from Mycobacterium bovis cells fed with 4-thiouridine and crosslinked via photoactivation at 3...
ORGANISM(S): Mycobacterium bovis 
2019-04-01 | PXD012661 | Pride
Tuberculosis Rna degradosome Rna bound proteome 
ProteomicsIdentification of proteins associated with the human mitochondrial degradosome
The human mitochondrial degradosome is a complex of the ribonuclease PNPase (Q8TCS8, encoded by PNPT1 gene) and RNA helicase SUV3 (Q8IYB8, encoded by SUPV3L1 gene). The aim of the project was to identify proteins which co-purify with both subunits of the degradosome. To this end we used human 293 ce...
ORGANISM(S): Homo sapiens (Human) 
2018-07-06 | PXD009826 | Pride
Mitochondrial rna Supv3l1 Suv3 Pnpt1 Mitochondrial degradosome Pnpase 
ProteomicsTENT5A mediated cytoplasmic polyadenylation in osteoblast supports proper bone formation.
The bone formation, osteogenesis, is a very complex process in which osteoblasts play a crucial role. The primary function of theses terminally differentiated mesenchymal stem cells is the secretion of fibrillary, dense, highly crosslinked type 1 collagen. Herein we show that the TENT5A gene, which...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-16 | PXD020346 | Pride
Collagen Mice Lc-ms/ms 
ProteomicsRNA degradosome components purified from Mycobacterium tuberculosis
The dataset accompanying a publication describing the RNA degradosome components in Mycobacterium tuberculosis (Mtb). Individual RNA degradosome components from Mtb (PNPase, RNase E, RNase J, RhlE and Enolase) were tagged with eGFP and used as baits to pull-down their interaction partners. The prote...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2019-04-01 | PXD012662 | Pride
Tuberculosis Rna degradation 
ProteomicsDIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice.
Among numerous enzymes involved in RNA decay, processive exoribonucleases are the most prominent group responsible for the degradation of the entire RNA molecules. The role of mammalian cytoplasmic 3’-5’ exonuclease DIS3L at the organismal level remained unknown. Herein we established knock-in and k...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD038745 | Pride
Dis3l Rna 
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