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ProteomicsHigh-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform
Mass spectrometry has transformed the field of cell signalling by enabling global studies of dynamic protein phosphorylation (‘phosphoproteomics’). Recent developments are enabling increasingly sophisticated phosphoproteomics studies, but practical challenges remain. The EasyPhos workflow addres...
ORGANISM(S): Homo sapiens (Human) 
2018-09-07 | PXD009227 | Pride
Glioblastoma Cancer Proteomics Post-translational modification Signal transduction Mass spectrometry Phosphorylation Egf Phosphoproteomics 
ProteomicsPhosphoproteomic study of Humanin treatment on human brain tumour cells
Humanin (HN) is a 24 amino acid peptide encoded by mitochondrial DNA MT-RNR2 (16S ribosomal RNA [rRNA]). It was previously shown, that HN protects tumor cells from damage during chemotherapy. To get mechanistic insight of HN induced singaling cascades involved in brain tumor growth, we performed a g...
ORGANISM(S): Homo sapiens (Human) 
2024-07-15 | PXD051404 | Pride
Glioblastoma Humanin Brain tumour 
ProteomicsSleep-wake cycles drive daily dynamics of synaptic phosphorylation
The circadian clock drives daily changes of physiology, including sleep-wake cycles, by regulating transcription, protein abundance and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We...
ORGANISM(S): Mus musculus (Mouse) 
2019-10-14 | PXD010697 | Pride
Synaptoneurosome Circadian Sleep Mass spectrometry Phosphorylation Easyphos Phosphoproteomics 
ProteomicsDiscovery of drugs to treat cytokine storm-induced cardiac dysfunction
SARS-CoV2 infection leads to cardiac injury and dysfunction in 20-30% of hospitalized patients and higher rates of mortality in patients with pre-existing cardiovascular disease. Inflammatory factors released as part of the 'cytokine storm' are thought to play a critical role in cardiac dysfunction ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-21 | PXD020994 | Pride
Sars-cov2 Cardiac organoids Cytokine storm Easyphos Phosphoproteomics Covid-19 Heart 
OtherHigh-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.
Data from ProteomeXchange, PXD ID: PXD001792. File: 2_QEp2_LC11_SeHu_LivPhos_Insulin_10m_Bio3.mzml. Published as part of Nat Biotechnol. 2015 Aug 17 . From the Abstract: {{i}} Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-t...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32320017798 | GPMDB
Resource Reanalysis 
ProteomicsMulti-Omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency
Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem-cell states. The two states that bookend the pluripotency continuum, naïve and primed, are well characterized, but our understanding of the intermediate states and transitions between them remain incomplete. Here, ...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-07 | PXD010621 | Pride
Stem cell Post-translational modification Differentiation Phosphoproteomics Embryonic Epilc Proteomics Pluripotency Dynamic Signal transduction Mass spectrometry Phosphorylation Easyphos Mesc 
ProteomicsDifferential cell survival outcomes in response to diverse amino acid stress
Mammalian cells respond to amino acid limitation by activating a coordinated response to preserve cell function and resources. Here we dissected amino acid stress sensing and adaptation and examined how prolonged arginine or leucine starvation alters cellular behavior of murine embryonic stem cells,...
ORGANISM(S): Mus musculus (Mouse) Saccharomyces cerevisiae (Baker's yeast) 
2025-04-01 | PXD030959 | Pride
Post-translational modification Proteomics Stress Phosphoproteomics Tube-ms Ap-ms Ubiquitinomics Castor1 Phosphorylation Protein-protein interaction Amino acid starvation Sesn2 Mtor 
ProteomicsPhosphoproteomic analysis of the adaption of epididymal epithelial cells to corticosterone challenge
Background: The epididymis has long been of interest owing to its role in promoting the functional maturation of the male germline. More recent evidence has also implicated the epididymis as an important sensory tissue responsible for remodeling of the sperm epigenome, both under physiological condi...
ORGANISM(S): Mus musculus (Mouse) 
2024-04-08 | PXD028789 | Pride
Proteomics Cellular signaling Stress response Corticosterone Sperm maturation Phosphoproteomics Epididymis 
ProteomicsHigh-throughput phosphoproteomics reveals in vivo insulin signaling dynamics
In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and p...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-06-25 | MSV000082502 | MassIVE
ProteomicsPhosphoproteomics sample preparation impacts biological interpretation of signalling responses
Quantitative data-dependent acquisition phosphoproteomics (DDAP) has revolutionised cell signaling studies. However, the impact of the specific DDAP sample preparation method on the phosphoproteome quantified is unclear as each study quantifies only a fraction of the phosphoproteome. Here, we compar...
ORGANISM(S): Mus musculus (Mouse) 
2021-12-25 | PXD025376 | Pride
Phosphoproteomics Tio2 Mouse High-throughput phosphoproteomics Scxphos Sample preparation Highphos Comparative analysis 
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